Nanoparticles Enhanced Self-Driven Microfludic Biosensor
C-reactive protein (CRP) plays an important role in inflammation detection and disease monitoring. The optical biosensor is a highly sensitive and easy detection tool. The microfluidic self-driving optical sensors were fabricated with transparent glass material and used for the enhanced surface plas...
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-03-01
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| Series: | Micromachines |
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| Online Access: | https://www.mdpi.com/2072-666X/11/4/350 |
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| author | Chunxiu Liu Ning Xue Haoyuan Cai Jianhai Sun Zhimei Qi Peiyue Zhao Fei Xiong Zhaoxin Geng Liying Jiang Li Li |
| author_facet | Chunxiu Liu Ning Xue Haoyuan Cai Jianhai Sun Zhimei Qi Peiyue Zhao Fei Xiong Zhaoxin Geng Liying Jiang Li Li |
| author_sort | Chunxiu Liu |
| collection | DOAJ |
| description | C-reactive protein (CRP) plays an important role in inflammation detection and disease monitoring. The optical biosensor is a highly sensitive and easy detection tool. The microfluidic self-driving optical sensors were fabricated with transparent glass material and used for the enhanced surface plasmon resonance (SPR) optical detection of the model protein CRP using Au nanoparticles (AuNPs) and a sandwich immune reaction. The 3D design of the chip was devised to improve the optical coupling efficiency and enable integration with a microfluidic control and rapid detection. The array of pre-fixed antibody modified by Au nanoparticles was used to achieve rapid antigen capture and improve the optical sensitivity. The Au nanoparticle amplification approach was introduced for the SPR detection of a target protein. CRP was used as a model target protein as part of a sandwich assay. The use of Au NP measurements to detect the target signal is a threefold improvement compared to single SPR detection methods. |
| first_indexed | 2024-03-11T10:12:22Z |
| format | Article |
| id | doaj.art-f845272144c44dd9a7ff708008434862 |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-03-11T10:12:22Z |
| publishDate | 2020-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-f845272144c44dd9a7ff7080084348622023-11-16T14:29:30ZengMDPI AGMicromachines2072-666X2020-03-0111435010.3390/mi11040350Nanoparticles Enhanced Self-Driven Microfludic BiosensorChunxiu Liu0Ning Xue1Haoyuan Cai2Jianhai Sun3Zhimei Qi4Peiyue Zhao5Fei Xiong6Zhaoxin Geng7Liying Jiang8Li Li9State Key Laboratory of Transducer Technology, Aerospace Information Research Institute, Chinese Academy of Sciences, Beijing 100190, ChinaState Key Laboratory of Transducer Technology, Aerospace Information Research Institute, Chinese Academy of Sciences, Beijing 100190, ChinaState Key Laboratory of Transducer Technology, Aerospace Information Research Institute, Chinese Academy of Sciences, Beijing 100190, ChinaState Key Laboratory of Transducer Technology, Aerospace Information Research Institute, Chinese Academy of Sciences, Beijing 100190, ChinaState Key Laboratory of Transducer Technology, Aerospace Information Research Institute, Chinese Academy of Sciences, Beijing 100190, ChinaState Key Laboratory of Transducer Technology, Aerospace Information Research Institute, Chinese Academy of Sciences, Beijing 100190, ChinaState Key Laboratory of Transducer Technology, Aerospace Information Research Institute, Chinese Academy of Sciences, Beijing 100190, ChinaSchool of Information Engineering, Minzu University of China, Beijing 100049, ChinaCollege of Electrical and Information Engineering, Zhengzhou University of Light Industry, Zhengzhou 450002, ChinaCollege of Land Science and Technology, China Agricultural University, Beijing 100083, ChinaC-reactive protein (CRP) plays an important role in inflammation detection and disease monitoring. The optical biosensor is a highly sensitive and easy detection tool. The microfluidic self-driving optical sensors were fabricated with transparent glass material and used for the enhanced surface plasmon resonance (SPR) optical detection of the model protein CRP using Au nanoparticles (AuNPs) and a sandwich immune reaction. The 3D design of the chip was devised to improve the optical coupling efficiency and enable integration with a microfluidic control and rapid detection. The array of pre-fixed antibody modified by Au nanoparticles was used to achieve rapid antigen capture and improve the optical sensitivity. The Au nanoparticle amplification approach was introduced for the SPR detection of a target protein. CRP was used as a model target protein as part of a sandwich assay. The use of Au NP measurements to detect the target signal is a threefold improvement compared to single SPR detection methods.https://www.mdpi.com/2072-666X/11/4/350microfluidic biosensoramplification effect of nanoparticleoptomagnetic detectionsandwich assay |
| spellingShingle | Chunxiu Liu Ning Xue Haoyuan Cai Jianhai Sun Zhimei Qi Peiyue Zhao Fei Xiong Zhaoxin Geng Liying Jiang Li Li Nanoparticles Enhanced Self-Driven Microfludic Biosensor Micromachines microfluidic biosensor amplification effect of nanoparticle optomagnetic detection sandwich assay |
| title | Nanoparticles Enhanced Self-Driven Microfludic Biosensor |
| title_full | Nanoparticles Enhanced Self-Driven Microfludic Biosensor |
| title_fullStr | Nanoparticles Enhanced Self-Driven Microfludic Biosensor |
| title_full_unstemmed | Nanoparticles Enhanced Self-Driven Microfludic Biosensor |
| title_short | Nanoparticles Enhanced Self-Driven Microfludic Biosensor |
| title_sort | nanoparticles enhanced self driven microfludic biosensor |
| topic | microfluidic biosensor amplification effect of nanoparticle optomagnetic detection sandwich assay |
| url | https://www.mdpi.com/2072-666X/11/4/350 |
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