First insights into the genetic diversity of <it>Mycobacterium tuberculosis </it>isolates from HIV-infected Mexican patients and mutations causing multidrug resistance
<p>Abstract</p> <p>Background</p> <p>The prevalence of infections with <it>Mycobacterium tuberculosis </it>(MTb) and nontuberculous mycobacteria (NTM) species in HIV-infected patients in Mexico is unknown. The aims of this study were to determine the frequen...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2010-03-01
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| Series: | BMC Microbiology |
| Online Access: | http://www.biomedcentral.com/1471-2180/10/82 |
| _version_ | 1811260330273144832 |
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| author | Hernandez-Pando Rogelio Aguilar Diana Helguera-Repetto Addy C Rivera-Gutierrez Sandra Cerna-Cortes Jorge F Castillo-Ramirez Ivan Badillo-Lopez Claudia Lopez-Alvarez Rocio Samper Sofia Gonzalez-y-Merchand Jorge A |
| author_facet | Hernandez-Pando Rogelio Aguilar Diana Helguera-Repetto Addy C Rivera-Gutierrez Sandra Cerna-Cortes Jorge F Castillo-Ramirez Ivan Badillo-Lopez Claudia Lopez-Alvarez Rocio Samper Sofia Gonzalez-y-Merchand Jorge A |
| author_sort | Hernandez-Pando Rogelio |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The prevalence of infections with <it>Mycobacterium tuberculosis </it>(MTb) and nontuberculous mycobacteria (NTM) species in HIV-infected patients in Mexico is unknown. The aims of this study were to determine the frequency of MTb and NTM species in HIV-infected patients from Mexico City, to evaluate the genotypic diversity of the <it>Mycobacterium tuberculosis </it>complex strains, to determine their drug resistance profiles by colorimetric microplate Alamar Blue assay (MABA), and finally, to detect mutations present in <it>kat</it>G, <it>rpo</it>B and <it>inh</it>A genes, resulting in isoniazid (INH) and rifampin (RIF) resistance.</p> <p>Results</p> <p>Of the 67 mycobacterial strains isolated, 48 were identified as MTb, 9 as <it>M. bovis</it>, 9 as <it>M. avium </it>and 1 as <it>M. intracellulare</it>. IS<it>6110</it>-RFLP of 48 MTb strains showed 27 profiles. Spoligotyping of the 48 MTb strains yielded 21 patterns, and 9 <it>M. bovis </it>strains produced 7 patterns. Eleven new spoligotypes patterns were found. A total of 40 patterns were produced from the 48 MTb strains when MIRU-VNTR was performed. Nineteen (39.6%) MTb strains were resistant to one or more drugs. One (2.1%) multidrug-resistant (MDR) strain was identified. A novel mutation was identified in a RIF-resistant strain, GAG → TCG (Glu → Ser) at codon 469 of <it>rpo</it>B gene.</p> <p>Conclusions</p> <p>This is the first molecular analysis of mycobacteria isolated from HIV-infected patients in Mexico, which describe the prevalence of different mycobacterial species in this population. A high genetic diversity of MTb strains was identified. New spoligotypes and MIRU-VNTR patterns as well as a novel mutation associated to RIF-resistance were found. This information will facilitate the tracking of different mycobacterial species in HIV-infected individuals, and monitoring the spread of these microorganisms, leading to more appropriate measures for tuberculosis control.</p> |
| first_indexed | 2024-04-12T18:45:51Z |
| format | Article |
| id | doaj.art-f84aa7ec539e456f92d3c39919ac2011 |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-04-12T18:45:51Z |
| publishDate | 2010-03-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-f84aa7ec539e456f92d3c39919ac20112022-12-22T03:20:37ZengBMCBMC Microbiology1471-21802010-03-011018210.1186/1471-2180-10-82First insights into the genetic diversity of <it>Mycobacterium tuberculosis </it>isolates from HIV-infected Mexican patients and mutations causing multidrug resistanceHernandez-Pando RogelioAguilar DianaHelguera-Repetto Addy CRivera-Gutierrez SandraCerna-Cortes Jorge FCastillo-Ramirez IvanBadillo-Lopez ClaudiaLopez-Alvarez RocioSamper SofiaGonzalez-y-Merchand Jorge A<p>Abstract</p> <p>Background</p> <p>The prevalence of infections with <it>Mycobacterium tuberculosis </it>(MTb) and nontuberculous mycobacteria (NTM) species in HIV-infected patients in Mexico is unknown. The aims of this study were to determine the frequency of MTb and NTM species in HIV-infected patients from Mexico City, to evaluate the genotypic diversity of the <it>Mycobacterium tuberculosis </it>complex strains, to determine their drug resistance profiles by colorimetric microplate Alamar Blue assay (MABA), and finally, to detect mutations present in <it>kat</it>G, <it>rpo</it>B and <it>inh</it>A genes, resulting in isoniazid (INH) and rifampin (RIF) resistance.</p> <p>Results</p> <p>Of the 67 mycobacterial strains isolated, 48 were identified as MTb, 9 as <it>M. bovis</it>, 9 as <it>M. avium </it>and 1 as <it>M. intracellulare</it>. IS<it>6110</it>-RFLP of 48 MTb strains showed 27 profiles. Spoligotyping of the 48 MTb strains yielded 21 patterns, and 9 <it>M. bovis </it>strains produced 7 patterns. Eleven new spoligotypes patterns were found. A total of 40 patterns were produced from the 48 MTb strains when MIRU-VNTR was performed. Nineteen (39.6%) MTb strains were resistant to one or more drugs. One (2.1%) multidrug-resistant (MDR) strain was identified. A novel mutation was identified in a RIF-resistant strain, GAG → TCG (Glu → Ser) at codon 469 of <it>rpo</it>B gene.</p> <p>Conclusions</p> <p>This is the first molecular analysis of mycobacteria isolated from HIV-infected patients in Mexico, which describe the prevalence of different mycobacterial species in this population. A high genetic diversity of MTb strains was identified. New spoligotypes and MIRU-VNTR patterns as well as a novel mutation associated to RIF-resistance were found. This information will facilitate the tracking of different mycobacterial species in HIV-infected individuals, and monitoring the spread of these microorganisms, leading to more appropriate measures for tuberculosis control.</p>http://www.biomedcentral.com/1471-2180/10/82 |
| spellingShingle | Hernandez-Pando Rogelio Aguilar Diana Helguera-Repetto Addy C Rivera-Gutierrez Sandra Cerna-Cortes Jorge F Castillo-Ramirez Ivan Badillo-Lopez Claudia Lopez-Alvarez Rocio Samper Sofia Gonzalez-y-Merchand Jorge A First insights into the genetic diversity of <it>Mycobacterium tuberculosis </it>isolates from HIV-infected Mexican patients and mutations causing multidrug resistance BMC Microbiology |
| title | First insights into the genetic diversity of <it>Mycobacterium tuberculosis </it>isolates from HIV-infected Mexican patients and mutations causing multidrug resistance |
| title_full | First insights into the genetic diversity of <it>Mycobacterium tuberculosis </it>isolates from HIV-infected Mexican patients and mutations causing multidrug resistance |
| title_fullStr | First insights into the genetic diversity of <it>Mycobacterium tuberculosis </it>isolates from HIV-infected Mexican patients and mutations causing multidrug resistance |
| title_full_unstemmed | First insights into the genetic diversity of <it>Mycobacterium tuberculosis </it>isolates from HIV-infected Mexican patients and mutations causing multidrug resistance |
| title_short | First insights into the genetic diversity of <it>Mycobacterium tuberculosis </it>isolates from HIV-infected Mexican patients and mutations causing multidrug resistance |
| title_sort | first insights into the genetic diversity of it mycobacterium tuberculosis it isolates from hiv infected mexican patients and mutations causing multidrug resistance |
| url | http://www.biomedcentral.com/1471-2180/10/82 |
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