Towards Automated and High-Throughput Quantitative Sizing and Isotopic Analysis of Nanoparticles via Single Particle-ICP-TOF-MS
The work described herein assesses the ability to characterize gold nanoparticles (Au NPs) of 50 and 100 nm, as well as 60 nm silver shelled gold core nanospheres (Au/Ag NPs), for their mass, respective size, and isotopic composition in an automated and unattended fashion. Here, an innovative autosa...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-04-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/13/8/1322 |
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| author | Benjamin T. Manard Veronica C. Bradley C. Derrick Quarles Lyndsey Hendriks Daniel R. Dunlap Cole R. Hexel Patrick Sullivan Hunter B. Andrews |
| author_facet | Benjamin T. Manard Veronica C. Bradley C. Derrick Quarles Lyndsey Hendriks Daniel R. Dunlap Cole R. Hexel Patrick Sullivan Hunter B. Andrews |
| author_sort | Benjamin T. Manard |
| collection | DOAJ |
| description | The work described herein assesses the ability to characterize gold nanoparticles (Au NPs) of 50 and 100 nm, as well as 60 nm silver shelled gold core nanospheres (Au/Ag NPs), for their mass, respective size, and isotopic composition in an automated and unattended fashion. Here, an innovative autosampler was employed to mix and transport the blanks, standards, and samples into a high-efficiency single particle (SP) introduction system for subsequent analysis by inductively coupled plasma–time of flight–mass spectrometry (ICP-TOF-MS). Optimized NP transport efficiency into the ICP-TOF-MS was determined to be >80%. This combination, SP-ICP-TOF-MS, allowed for high-throughput sample analysis. Specifically, 50 total samples (including blanks/standards) were analyzed over 8 h, to provide an accurate characterization of the NPs. This methodology was implemented over the course of 5 days to assess its long-term reproducibility. Impressively, the in-run and day-to-day variation of sample transport is assessed to be 3.54 and 9.52% relative standard deviation (%RSD), respectively. The determination of Au NP size and concentration was of <5% relative difference from the certified values over these time periods. Isotopic characterization of the <sup>107</sup>Ag/<sup>109</sup>Ag particles (<i>n</i> = 132,630) over the course of the measurements was determined to be 1.0788 ± 0.0030 with high accuracy (0.23% relative difference) when compared to the multi-collector–ICP-MS determination. |
| first_indexed | 2024-03-11T04:40:54Z |
| format | Article |
| id | doaj.art-f84d438feeec4cb1972d661c2ef78d25 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-11T04:40:54Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-f84d438feeec4cb1972d661c2ef78d252023-11-17T20:42:48ZengMDPI AGNanomaterials2079-49912023-04-01138132210.3390/nano13081322Towards Automated and High-Throughput Quantitative Sizing and Isotopic Analysis of Nanoparticles via Single Particle-ICP-TOF-MSBenjamin T. Manard0Veronica C. Bradley1C. Derrick Quarles2Lyndsey Hendriks3Daniel R. Dunlap4Cole R. Hexel5Patrick Sullivan6Hunter B. Andrews7Chemical Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831, USAChemical Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831, USAElemental Scientific, Inc., Omaha, NE 68122, USATOFWERK AG, 3645 Thun, SwitzerlandChemical Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831, USAChemical Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831, USAElemental Scientific, Inc., Omaha, NE 68122, USARadioisotope Science and Technology Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831, USAThe work described herein assesses the ability to characterize gold nanoparticles (Au NPs) of 50 and 100 nm, as well as 60 nm silver shelled gold core nanospheres (Au/Ag NPs), for their mass, respective size, and isotopic composition in an automated and unattended fashion. Here, an innovative autosampler was employed to mix and transport the blanks, standards, and samples into a high-efficiency single particle (SP) introduction system for subsequent analysis by inductively coupled plasma–time of flight–mass spectrometry (ICP-TOF-MS). Optimized NP transport efficiency into the ICP-TOF-MS was determined to be >80%. This combination, SP-ICP-TOF-MS, allowed for high-throughput sample analysis. Specifically, 50 total samples (including blanks/standards) were analyzed over 8 h, to provide an accurate characterization of the NPs. This methodology was implemented over the course of 5 days to assess its long-term reproducibility. Impressively, the in-run and day-to-day variation of sample transport is assessed to be 3.54 and 9.52% relative standard deviation (%RSD), respectively. The determination of Au NP size and concentration was of <5% relative difference from the certified values over these time periods. Isotopic characterization of the <sup>107</sup>Ag/<sup>109</sup>Ag particles (<i>n</i> = 132,630) over the course of the measurements was determined to be 1.0788 ± 0.0030 with high accuracy (0.23% relative difference) when compared to the multi-collector–ICP-MS determination.https://www.mdpi.com/2079-4991/13/8/1322nanoparticlesautomatedhigh throughputsingle particleICP-TOF-MSSP-ICP-MS |
| spellingShingle | Benjamin T. Manard Veronica C. Bradley C. Derrick Quarles Lyndsey Hendriks Daniel R. Dunlap Cole R. Hexel Patrick Sullivan Hunter B. Andrews Towards Automated and High-Throughput Quantitative Sizing and Isotopic Analysis of Nanoparticles via Single Particle-ICP-TOF-MS Nanomaterials nanoparticles automated high throughput single particle ICP-TOF-MS SP-ICP-MS |
| title | Towards Automated and High-Throughput Quantitative Sizing and Isotopic Analysis of Nanoparticles via Single Particle-ICP-TOF-MS |
| title_full | Towards Automated and High-Throughput Quantitative Sizing and Isotopic Analysis of Nanoparticles via Single Particle-ICP-TOF-MS |
| title_fullStr | Towards Automated and High-Throughput Quantitative Sizing and Isotopic Analysis of Nanoparticles via Single Particle-ICP-TOF-MS |
| title_full_unstemmed | Towards Automated and High-Throughput Quantitative Sizing and Isotopic Analysis of Nanoparticles via Single Particle-ICP-TOF-MS |
| title_short | Towards Automated and High-Throughput Quantitative Sizing and Isotopic Analysis of Nanoparticles via Single Particle-ICP-TOF-MS |
| title_sort | towards automated and high throughput quantitative sizing and isotopic analysis of nanoparticles via single particle icp tof ms |
| topic | nanoparticles automated high throughput single particle ICP-TOF-MS SP-ICP-MS |
| url | https://www.mdpi.com/2079-4991/13/8/1322 |
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