Quantitative proteomics by iTRAQ-PRM based reveals the new characterization for gout
Abstract Background Gout is a common and complex form of immunoreactive arthritis based on hyperuricemia, while the symptoms would turn to remission or even got worse. So, it is hard to early identify whether an asymptomatic hyperuricemia (AHU) patient will be susceptible to get acute gout attack an...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2021-10-01
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| Series: | Proteome Science |
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| Online Access: | https://doi.org/10.1186/s12953-021-00180-0 |
| _version_ | 1818741343669714944 |
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| author | Guangqi Chen Jiafen Cheng Hanjie Yu Xiao Huang Hui Bao Ling Qin Ling Wang Yaxiang Song Xinying Liu Ai Peng |
| author_facet | Guangqi Chen Jiafen Cheng Hanjie Yu Xiao Huang Hui Bao Ling Qin Ling Wang Yaxiang Song Xinying Liu Ai Peng |
| author_sort | Guangqi Chen |
| collection | DOAJ |
| description | Abstract Background Gout is a common and complex form of immunoreactive arthritis based on hyperuricemia, while the symptoms would turn to remission or even got worse. So, it is hard to early identify whether an asymptomatic hyperuricemia (AHU) patient will be susceptible to get acute gout attack and it is also hard to predict the process of gout remission to flare. Here, we report that the plasma proteins profile can distinguish among acute gout (AG), remission of gout (RG), AHU patients, and healthy controls. Methods We established an isobaric tags for relative and absolute quantification (iTRAQ) and parallel reaction monitoring (PRM) based method to measure the plasma proteins for AG group (n = 8), RG group (n = 7), AHU group (n = 7) and healthy controls (n = 8). Results Eleven differentially expressed proteins such as Histone H2A, Histone H2B, Thrombospondin-1 (THBS1), Myeloperoxidase (MPO), Complement C2, Complement component C8 beta chain (C8B), Alpha-1-acid glycoprotein 1 (ORM1), Inter-alpha-trypsin inhibitor heavy chain H4 (ITIH4), Carbonic anhydrase 1 (CA1), Serum albumin (ALB) and Multimerin-1 (MMRN1) were identified. Histone H2A, Histone H2B and THBS1 might be the strongest influential regulator to maintain the balance and stability of the gout process. The complement and coagulation cascades is one of the main functional pathways in the mechanism of gout process. Conclusions Histone H2A, Histone H2B and THBS1 are potential candidate genes for novel biomarkers in discriminating gout attack from AHU or RG, providing new theoretical insights for the prognosis, treatment, and management of gout process. Trial registration This study is not a clinical trial. |
| first_indexed | 2024-12-18T01:55:07Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1477-5956 |
| language | English |
| last_indexed | 2024-12-18T01:55:07Z |
| publishDate | 2021-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Proteome Science |
| spelling | doaj.art-f851b66c91dd48efa76afe79c71ef3902022-12-21T21:24:56ZengBMCProteome Science1477-59562021-10-0119111310.1186/s12953-021-00180-0Quantitative proteomics by iTRAQ-PRM based reveals the new characterization for goutGuangqi Chen0Jiafen Cheng1Hanjie Yu2Xiao Huang3Hui Bao4Ling Qin5Ling Wang6Yaxiang Song7Xinying Liu8Ai Peng9Center for Nephrology and Clinical Metabolomics and Division of Nephrology and Rheumatology, Shanghai Tenth People’s Hospital, Tongji University School of MedicineCenter for Nephrology and Clinical Metabolomics and Division of Nephrology and Rheumatology, Shanghai Tenth People’s Hospital, Tongji University School of MedicineCenter for Nephrology and Clinical Metabolomics and Division of Nephrology and Rheumatology, Shanghai Tenth People’s Hospital, Tongji University School of MedicineCenter for Nephrology and Clinical Metabolomics and Division of Nephrology and Rheumatology, Shanghai Tenth People’s Hospital, Tongji University School of MedicineCenter for Nephrology and Clinical Metabolomics and Division of Nephrology and Rheumatology, Shanghai Tenth People’s Hospital, Tongji University School of MedicineCenter for Nephrology and Clinical Metabolomics and Division of Nephrology and Rheumatology, Shanghai Tenth People’s Hospital, Tongji University School of MedicineCenter for Nephrology and Clinical Metabolomics and Division of Nephrology and Rheumatology, Shanghai Tenth People’s Hospital, Tongji University School of MedicineCenter for Nephrology and Clinical Metabolomics and Division of Nephrology and Rheumatology, Shanghai Tenth People’s Hospital, Tongji University School of MedicineCenter for Nephrology and Clinical Metabolomics and Division of Nephrology and Rheumatology, Shanghai Tenth People’s Hospital, Tongji University School of MedicineCenter for Nephrology and Clinical Metabolomics and Division of Nephrology and Rheumatology, Shanghai Tenth People’s Hospital, Tongji University School of MedicineAbstract Background Gout is a common and complex form of immunoreactive arthritis based on hyperuricemia, while the symptoms would turn to remission or even got worse. So, it is hard to early identify whether an asymptomatic hyperuricemia (AHU) patient will be susceptible to get acute gout attack and it is also hard to predict the process of gout remission to flare. Here, we report that the plasma proteins profile can distinguish among acute gout (AG), remission of gout (RG), AHU patients, and healthy controls. Methods We established an isobaric tags for relative and absolute quantification (iTRAQ) and parallel reaction monitoring (PRM) based method to measure the plasma proteins for AG group (n = 8), RG group (n = 7), AHU group (n = 7) and healthy controls (n = 8). Results Eleven differentially expressed proteins such as Histone H2A, Histone H2B, Thrombospondin-1 (THBS1), Myeloperoxidase (MPO), Complement C2, Complement component C8 beta chain (C8B), Alpha-1-acid glycoprotein 1 (ORM1), Inter-alpha-trypsin inhibitor heavy chain H4 (ITIH4), Carbonic anhydrase 1 (CA1), Serum albumin (ALB) and Multimerin-1 (MMRN1) were identified. Histone H2A, Histone H2B and THBS1 might be the strongest influential regulator to maintain the balance and stability of the gout process. The complement and coagulation cascades is one of the main functional pathways in the mechanism of gout process. Conclusions Histone H2A, Histone H2B and THBS1 are potential candidate genes for novel biomarkers in discriminating gout attack from AHU or RG, providing new theoretical insights for the prognosis, treatment, and management of gout process. Trial registration This study is not a clinical trial.https://doi.org/10.1186/s12953-021-00180-0GoutiTRAQPRMHistone H2AHistone H2BTHBS1 |
| spellingShingle | Guangqi Chen Jiafen Cheng Hanjie Yu Xiao Huang Hui Bao Ling Qin Ling Wang Yaxiang Song Xinying Liu Ai Peng Quantitative proteomics by iTRAQ-PRM based reveals the new characterization for gout Proteome Science Gout iTRAQ PRM Histone H2A Histone H2B THBS1 |
| title | Quantitative proteomics by iTRAQ-PRM based reveals the new characterization for gout |
| title_full | Quantitative proteomics by iTRAQ-PRM based reveals the new characterization for gout |
| title_fullStr | Quantitative proteomics by iTRAQ-PRM based reveals the new characterization for gout |
| title_full_unstemmed | Quantitative proteomics by iTRAQ-PRM based reveals the new characterization for gout |
| title_short | Quantitative proteomics by iTRAQ-PRM based reveals the new characterization for gout |
| title_sort | quantitative proteomics by itraq prm based reveals the new characterization for gout |
| topic | Gout iTRAQ PRM Histone H2A Histone H2B THBS1 |
| url | https://doi.org/10.1186/s12953-021-00180-0 |
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