Isolation and characterization of anti-proliferative and anti-oxidative mannan from Saccharomyces cerevisiae
Mannan oligosaccharide is one of the major components of the yeast cell wall. In the present study, the production, characterization, and bioactivity of yeast mannan were investigated. Four indigenous yeast isolates were obtained from various kinds of juice collected from local markets in Al-Ahsa, K...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-02-01
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| Series: | Journal of King Saud University: Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1018364721004365 |
| _version_ | 1798025193077080064 |
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| author | Ramy S. Yehia Ahmed M. Saleh Mohammad Bani Ismail Saleh Al-Quraishy Ohoud Al-Amri Rewaida Abdel-Gaber |
| author_facet | Ramy S. Yehia Ahmed M. Saleh Mohammad Bani Ismail Saleh Al-Quraishy Ohoud Al-Amri Rewaida Abdel-Gaber |
| author_sort | Ramy S. Yehia |
| collection | DOAJ |
| description | Mannan oligosaccharide is one of the major components of the yeast cell wall. In the present study, the production, characterization, and bioactivity of yeast mannan were investigated. Four indigenous yeast isolates were obtained from various kinds of juice collected from local markets in Al-Ahsa, KSA, and analyzed using morphological and biochemical methods. Isolate RY1 showed the highest production of mannan. RY1 was identified as Saccharomyces cerevisiae based on morphological characteristics and sequencing of the 18S rRNA gene (GenBank accession number LC479088.1). Mannan-RY1 was characterized by polymer analytical methods, 13C and 1H nuclear magnetic resonance spectroscopy (NMR), and infrared spectroscopy (IR). Interestingly, the mannan extracted from RY1 showed a significant ability to scavenge hydroxyl radicals and superoxide anions. In addition, mannan was found to have pronounced anti-tumor activity against liver (HepG2) and breast cancer (MCF7) cell lines. The results presented in this study confirm the bio-therapeutic activity of S. cerevisiae mannan, which could be used as a potential drug for cancer treatment. |
| first_indexed | 2024-04-11T18:15:51Z |
| format | Article |
| id | doaj.art-f851ef0ca8444943b47f3002d5b00a9f |
| institution | Directory Open Access Journal |
| issn | 1018-3647 |
| language | English |
| last_indexed | 2024-04-11T18:15:51Z |
| publishDate | 2022-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of King Saud University: Science |
| spelling | doaj.art-f851ef0ca8444943b47f3002d5b00a9f2022-12-22T04:09:57ZengElsevierJournal of King Saud University: Science1018-36472022-02-01342101774Isolation and characterization of anti-proliferative and anti-oxidative mannan from Saccharomyces cerevisiaeRamy S. Yehia0Ahmed M. Saleh1Mohammad Bani Ismail2Saleh Al-Quraishy3Ohoud Al-Amri4Rewaida Abdel-Gaber5Department of Biological Sciences, College of Science, King Faisal University, Al-Ahsa, Saudi Arabia; Department of Botany and Microbiology, Faculty of Science, Cairo University, Giza, EgyptDepartment of Botany and Microbiology, Faculty of Science, Cairo University, Giza, EgyptDepartment of Biological Sciences, College of Science, King Faisal University, Al-Ahsa, Saudi ArabiaDepartment of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia; Corresponding author.Department of Zoology, College of Science, King Saud University, Riyadh, Saudi ArabiaDepartment of Zoology, College of Science, King Saud University, Riyadh, Saudi ArabiaMannan oligosaccharide is one of the major components of the yeast cell wall. In the present study, the production, characterization, and bioactivity of yeast mannan were investigated. Four indigenous yeast isolates were obtained from various kinds of juice collected from local markets in Al-Ahsa, KSA, and analyzed using morphological and biochemical methods. Isolate RY1 showed the highest production of mannan. RY1 was identified as Saccharomyces cerevisiae based on morphological characteristics and sequencing of the 18S rRNA gene (GenBank accession number LC479088.1). Mannan-RY1 was characterized by polymer analytical methods, 13C and 1H nuclear magnetic resonance spectroscopy (NMR), and infrared spectroscopy (IR). Interestingly, the mannan extracted from RY1 showed a significant ability to scavenge hydroxyl radicals and superoxide anions. In addition, mannan was found to have pronounced anti-tumor activity against liver (HepG2) and breast cancer (MCF7) cell lines. The results presented in this study confirm the bio-therapeutic activity of S. cerevisiae mannan, which could be used as a potential drug for cancer treatment.http://www.sciencedirect.com/science/article/pii/S1018364721004365MannanSaccharomyces cerevisiaeCytotoxicityAntioxidant |
| spellingShingle | Ramy S. Yehia Ahmed M. Saleh Mohammad Bani Ismail Saleh Al-Quraishy Ohoud Al-Amri Rewaida Abdel-Gaber Isolation and characterization of anti-proliferative and anti-oxidative mannan from Saccharomyces cerevisiae Journal of King Saud University: Science Mannan Saccharomyces cerevisiae Cytotoxicity Antioxidant |
| title | Isolation and characterization of anti-proliferative and anti-oxidative mannan from Saccharomyces cerevisiae |
| title_full | Isolation and characterization of anti-proliferative and anti-oxidative mannan from Saccharomyces cerevisiae |
| title_fullStr | Isolation and characterization of anti-proliferative and anti-oxidative mannan from Saccharomyces cerevisiae |
| title_full_unstemmed | Isolation and characterization of anti-proliferative and anti-oxidative mannan from Saccharomyces cerevisiae |
| title_short | Isolation and characterization of anti-proliferative and anti-oxidative mannan from Saccharomyces cerevisiae |
| title_sort | isolation and characterization of anti proliferative and anti oxidative mannan from saccharomyces cerevisiae |
| topic | Mannan Saccharomyces cerevisiae Cytotoxicity Antioxidant |
| url | http://www.sciencedirect.com/science/article/pii/S1018364721004365 |
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