Thickness of the aqueous boundary layer in stirred microtitre plate permeability assays (PAMPA and Caco-2), based on the Levich equation
The stirring frequency exponent of -1/2 in the theoretical Levich expression appears to apply to PAMPA and Caco-2 assays, where efficient individual-well magnetic stirring ( > 20 RPM) is used. If a single molecule is used as a stirring calibrant, then scaling suggested by Eq. (5) with microtitr...
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| Format: | Article |
| Language: | English |
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International Association of Physical Chemists (IAPC)
2022-12-01
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| Series: | ADMET and DMPK |
| Online Access: | http://pub.iapchem.org/ojs/index.php/admet/article/view/1568 |
| _version_ | 1811178033648762880 |
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| author | Alex Avdeef |
| author_facet | Alex Avdeef |
| author_sort | Alex Avdeef |
| collection | DOAJ |
| description |
The stirring frequency exponent of -1/2 in the theoretical Levich expression appears to apply to PAMPA and Caco-2 assays, where efficient individual-well magnetic stirring ( > 20 RPM) is used. If a single molecule is used as a stirring calibrant, then scaling suggested by Eq. (5) with microtitre plate data may be used. The error in calculating hABL based on unscaled hABLref can be as high as 30%. This is of practical importance in PAMPA, and perhaps cellular assays as well.
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| first_indexed | 2024-04-11T06:11:28Z |
| format | Article |
| id | doaj.art-f853b0f24d8b46f5a2d96d11652a49eb |
| institution | Directory Open Access Journal |
| issn | 1848-7718 |
| language | English |
| last_indexed | 2024-04-11T06:11:28Z |
| publishDate | 2022-12-01 |
| publisher | International Association of Physical Chemists (IAPC) |
| record_format | Article |
| series | ADMET and DMPK |
| spelling | doaj.art-f853b0f24d8b46f5a2d96d11652a49eb2022-12-22T04:41:14ZengInternational Association of Physical Chemists (IAPC)ADMET and DMPK1848-77182022-12-0110410.5599/admet.1568Thickness of the aqueous boundary layer in stirred microtitre plate permeability assays (PAMPA and Caco-2), based on the Levich equationAlex Avdeef0in-ADME Research The stirring frequency exponent of -1/2 in the theoretical Levich expression appears to apply to PAMPA and Caco-2 assays, where efficient individual-well magnetic stirring ( > 20 RPM) is used. If a single molecule is used as a stirring calibrant, then scaling suggested by Eq. (5) with microtitre plate data may be used. The error in calculating hABL based on unscaled hABLref can be as high as 30%. This is of practical importance in PAMPA, and perhaps cellular assays as well. http://pub.iapchem.org/ojs/index.php/admet/article/view/1568 |
| spellingShingle | Alex Avdeef Thickness of the aqueous boundary layer in stirred microtitre plate permeability assays (PAMPA and Caco-2), based on the Levich equation ADMET and DMPK |
| title | Thickness of the aqueous boundary layer in stirred microtitre plate permeability assays (PAMPA and Caco-2), based on the Levich equation |
| title_full | Thickness of the aqueous boundary layer in stirred microtitre plate permeability assays (PAMPA and Caco-2), based on the Levich equation |
| title_fullStr | Thickness of the aqueous boundary layer in stirred microtitre plate permeability assays (PAMPA and Caco-2), based on the Levich equation |
| title_full_unstemmed | Thickness of the aqueous boundary layer in stirred microtitre plate permeability assays (PAMPA and Caco-2), based on the Levich equation |
| title_short | Thickness of the aqueous boundary layer in stirred microtitre plate permeability assays (PAMPA and Caco-2), based on the Levich equation |
| title_sort | thickness of the aqueous boundary layer in stirred microtitre plate permeability assays pampa and caco 2 based on the levich equation |
| url | http://pub.iapchem.org/ojs/index.php/admet/article/view/1568 |
| work_keys_str_mv | AT alexavdeef thicknessoftheaqueousboundarylayerinstirredmicrotitreplatepermeabilityassayspampaandcaco2basedonthelevichequation |