In vivo effects of low dose prenatal bisphenol A exposure on adiposity in male and female ICR offspring
Background: Bisphenol A (BPA) is known to exhibit endocrine disrupting activities and is associated with adiposity. We examined the obesogenic effect of prenatal BPA exposure in the present study. Methods: Pregnant ICR mice were exposed to vehicle or BPA via the drinking water at a dose of 0.5 μg/kg...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-06-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651323004505 |
| _version_ | 1797833166857175040 |
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| author | Yin Zhuang Xiangying Sun Siting Deng Ya Wen Qiujin Xu Quanquan Guan |
| author_facet | Yin Zhuang Xiangying Sun Siting Deng Ya Wen Qiujin Xu Quanquan Guan |
| author_sort | Yin Zhuang |
| collection | DOAJ |
| description | Background: Bisphenol A (BPA) is known to exhibit endocrine disrupting activities and is associated with adiposity. We examined the obesogenic effect of prenatal BPA exposure in the present study. Methods: Pregnant ICR mice were exposed to vehicle or BPA via the drinking water at a dose of 0.5 μg/kg·d throughout the gestation. Obesity-related indexes were investigated in the 12-wk-old offspring. Primary mouse embryonic fibroblasts (MEFs) collected from treated embryos were used to test effects of BPA on adipocyte differentiation. Results: Offspring presented a significantly higher rate of weight gain than the control, with impaired insulin sensitivity and increased adipocyte size. Differentiation of MEFs from BPA-treated mice showed a higher propensity for the adipocyte commitment as well as up-regulation of genes enriched in lipid biosynthesis. TGF-β signaling pathway was found to modulate obesogenic effect of BPA in MEF model, but estrogen signaling pathway had no effect. Conclusions: The present study provides strong evidence of the association between prenatal exposure to low dose of BPA and a significant increase in body weight in the offspring mice with a critical role played by TGF-β signaling pathway. The potential interactions modulating the binding of BPA and TGF-β that activate its obesogenic effects need to be examined. |
| first_indexed | 2024-04-09T14:20:10Z |
| format | Article |
| id | doaj.art-f854ff98827945fcb7efa5b9bcac5a92 |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-04-09T14:20:10Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-f854ff98827945fcb7efa5b9bcac5a922023-05-05T04:39:44ZengElsevierEcotoxicology and Environmental Safety0147-65132023-06-01257114946In vivo effects of low dose prenatal bisphenol A exposure on adiposity in male and female ICR offspringYin Zhuang0Xiangying Sun1Siting Deng2Ya Wen3Qiujin Xu4Quanquan Guan5Center for Global Health, School of Public Health, Nanjing Medical University, Nanjing 211166, China; Key Laboratory of Modern Toxicology of Ministry of Education, School of Public Health, Nanjing Medical University, Nanjing 211166, ChinaCenter for Global Health, School of Public Health, Nanjing Medical University, Nanjing 211166, ChinaKey Laboratory of Modern Toxicology of Ministry of Education, School of Public Health, Nanjing Medical University, Nanjing 211166, China; State Key Laboratory of Reproductive Medicine and Offspring Health, School of Public Health, Nanjing Medical University, Nanjing 211166, ChinaKey Laboratory of Modern Toxicology of Ministry of Education, School of Public Health, Nanjing Medical University, Nanjing 211166, China; State Key Laboratory of Reproductive Medicine and Offspring Health, School of Public Health, Nanjing Medical University, Nanjing 211166, ChinaCenter for Global Health, School of Public Health, Nanjing Medical University, Nanjing 211166, China; State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environment Science, Beijing 100012, China; Correspondence to: State Key Laboratory of Environmental Criteria and Risk Assessment, ChineseResearch Academy of Environment Science, No. 8 Dayangfang, Chaoyang District, Beijing 100012, China.Key Laboratory of Modern Toxicology of Ministry of Education, School of Public Health, Nanjing Medical University, Nanjing 211166, China; State Key Laboratory of Reproductive Medicine and Offspring Health, School of Public Health, Nanjing Medical University, Nanjing 211166, China; Correspondence to: State Key Laboratory of Reproductive Medicine, Institute of Toxicology, Nanjing Medical University, 101 Longmian Road, Nanjing 211166, China.Background: Bisphenol A (BPA) is known to exhibit endocrine disrupting activities and is associated with adiposity. We examined the obesogenic effect of prenatal BPA exposure in the present study. Methods: Pregnant ICR mice were exposed to vehicle or BPA via the drinking water at a dose of 0.5 μg/kg·d throughout the gestation. Obesity-related indexes were investigated in the 12-wk-old offspring. Primary mouse embryonic fibroblasts (MEFs) collected from treated embryos were used to test effects of BPA on adipocyte differentiation. Results: Offspring presented a significantly higher rate of weight gain than the control, with impaired insulin sensitivity and increased adipocyte size. Differentiation of MEFs from BPA-treated mice showed a higher propensity for the adipocyte commitment as well as up-regulation of genes enriched in lipid biosynthesis. TGF-β signaling pathway was found to modulate obesogenic effect of BPA in MEF model, but estrogen signaling pathway had no effect. Conclusions: The present study provides strong evidence of the association between prenatal exposure to low dose of BPA and a significant increase in body weight in the offspring mice with a critical role played by TGF-β signaling pathway. The potential interactions modulating the binding of BPA and TGF-β that activate its obesogenic effects need to be examined.http://www.sciencedirect.com/science/article/pii/S0147651323004505Bisphenol APrenatal exposureOffspring obesityTGFβ |
| spellingShingle | Yin Zhuang Xiangying Sun Siting Deng Ya Wen Qiujin Xu Quanquan Guan In vivo effects of low dose prenatal bisphenol A exposure on adiposity in male and female ICR offspring Ecotoxicology and Environmental Safety Bisphenol A Prenatal exposure Offspring obesity TGFβ |
| title | In vivo effects of low dose prenatal bisphenol A exposure on adiposity in male and female ICR offspring |
| title_full | In vivo effects of low dose prenatal bisphenol A exposure on adiposity in male and female ICR offspring |
| title_fullStr | In vivo effects of low dose prenatal bisphenol A exposure on adiposity in male and female ICR offspring |
| title_full_unstemmed | In vivo effects of low dose prenatal bisphenol A exposure on adiposity in male and female ICR offspring |
| title_short | In vivo effects of low dose prenatal bisphenol A exposure on adiposity in male and female ICR offspring |
| title_sort | in vivo effects of low dose prenatal bisphenol a exposure on adiposity in male and female icr offspring |
| topic | Bisphenol A Prenatal exposure Offspring obesity TGFβ |
| url | http://www.sciencedirect.com/science/article/pii/S0147651323004505 |
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