A novel PCR-based method for high throughput prokaryotic expression of antimicrobial peptide genes

A novel PCR-based method for high throughput prokaryotic expression of antimicrobial peptide genes

<p>Abstract</p> <p>Background</p> <p>To facilitate the screening of large quantities of new antimicrobial peptides (AMPs), we describe a cost-effective method for high throughput prokaryotic expression of AMPs. EDDIE, an autoproteolytic mutant of the N-terminal autoprot...

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Bibliographic Details
Main Authors: Ke Tao, Liang Su, Huang Jin, Mao Han, Chen Jibao, Dong Caihua, Huang Junyan, Liu Shengyi, Kang Jianxiong, Liu Dongqi, Ma Xiangdong
Format: Article
Language:English
Published: BMC 2012-03-01
Series:BMC Biotechnology
Subjects:
antimicrobial peptide
high throughput
Npro
prokaryotic expression
Online Access:http://www.biomedcentral.com/1472-6750/12/10

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