Molecular Characterization of the First Reported Neoplagioglyphe megastomus (Digenea, Omphalometridae) in Poland
The diversity and taxonomy of metacercariae infecting freshwater amphipods of Poland are predominantly poorly known. During parasitological surveys of Gammarus pulex (Crustacea: Amphipoda) in the Pomeranian region of Poland in 2020 and 2021, some specimens of crustaceans were found to harbour met...
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Publishing House "Akademperiodyka"
2022-05-01
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| Series: | Zoodiversity |
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| Online Access: | http://ojs.akademperiodyka.org.ua/index.php/Zoodiversity/article/view/302 |
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| author | V. Sarabeev M. Ovcharenko A. Ahmed R. A. Sueiro J. M. Leiro |
| author_facet | V. Sarabeev M. Ovcharenko A. Ahmed R. A. Sueiro J. M. Leiro |
| author_sort | V. Sarabeev |
| collection | DOAJ |
| description | The diversity and taxonomy of metacercariae infecting freshwater amphipods of Poland
are predominantly poorly known. During parasitological surveys of Gammarus pulex (Crustacea:
Amphipoda) in the Pomeranian region of Poland in 2020 and 2021, some specimens of crustaceans were
found to harbour metacercariae. Out of seven observed localities, metacercariae were found in the stream
close to Krępa Słupska and the Lupawa River close to Smoldzino. They were morphologically studied
and sequenced using universal eukaryotic primers that amplify V4–V5 regions of 18S rRNA. The BLAST
analysis and phylogenetic reconstructions aid to define the affiliation of the revealed metacercariae within
the Omphalometridae Looss, 1899. The further differential analysis based on morphologic information
led to the conclusion that the metacercarial form studied herein represents Neoplagioglyphe megastomus
(Baer, 1943). The degree of morphometric variations of taxonomic important features in populations
from different geographic regions was followed up. The reported here form was characterized by a larger
body size that was affected by the processing methods used in the present and previous studies. To our
knowledge, this is the first report of Neoplagioglyphe megastomus in Poland and is the first molecular
characterization of the worm. |
| first_indexed | 2024-04-13T04:44:43Z |
| format | Article |
| id | doaj.art-f85d83e288e64d3894be4577fa6e1b31 |
| institution | Directory Open Access Journal |
| issn | 2707-725X 2707-7268 |
| language | English |
| last_indexed | 2024-04-13T04:44:43Z |
| publishDate | 2022-05-01 |
| publisher | Publishing House "Akademperiodyka" |
| record_format | Article |
| series | Zoodiversity |
| spelling | doaj.art-f85d83e288e64d3894be4577fa6e1b312022-12-22T03:01:54ZengPublishing House "Akademperiodyka"Zoodiversity2707-725X2707-72682022-05-0156210.15407/zoo2022.02.083302Molecular Characterization of the First Reported Neoplagioglyphe megastomus (Digenea, Omphalometridae) in PolandV. Sarabeev0M. Ovcharenko1A. Ahmed2R. A. Sueiro3J. M. Leiro4Department of Biology, Zaporizhzhia National University, Ukraine; Institute of Biology and Science of Earth, Pomeranian University in Słupsk, PolandInstitute of Biology and Science of Earth, Pomeranian University in Słupsk, Arciszewskiego 22b, 76200 Słupsk, PolandDepartment of Biology, Zaporizhzhia National University, UkraineDepartment of Microbiology and Parasitology, Institute of Research in Biological and Chemical Analysis, University of Santiago de Compostela, 15782 Santiago de Compostela, SpainDepartment of Microbiology and Parasitology, Institute of Research in Biological and Chemical Analysis, University of Santiago de Compostela, 15782 Santiago de Compostela, SpainThe diversity and taxonomy of metacercariae infecting freshwater amphipods of Poland are predominantly poorly known. During parasitological surveys of Gammarus pulex (Crustacea: Amphipoda) in the Pomeranian region of Poland in 2020 and 2021, some specimens of crustaceans were found to harbour metacercariae. Out of seven observed localities, metacercariae were found in the stream close to Krępa Słupska and the Lupawa River close to Smoldzino. They were morphologically studied and sequenced using universal eukaryotic primers that amplify V4–V5 regions of 18S rRNA. The BLAST analysis and phylogenetic reconstructions aid to define the affiliation of the revealed metacercariae within the Omphalometridae Looss, 1899. The further differential analysis based on morphologic information led to the conclusion that the metacercarial form studied herein represents Neoplagioglyphe megastomus (Baer, 1943). The degree of morphometric variations of taxonomic important features in populations from different geographic regions was followed up. The reported here form was characterized by a larger body size that was affected by the processing methods used in the present and previous studies. To our knowledge, this is the first report of Neoplagioglyphe megastomus in Poland and is the first molecular characterization of the worm.http://ojs.akademperiodyka.org.ua/index.php/Zoodiversity/article/view/30218s rrnagammarus pulexpomeranian regionmetacercaria |
| spellingShingle | V. Sarabeev M. Ovcharenko A. Ahmed R. A. Sueiro J. M. Leiro Molecular Characterization of the First Reported Neoplagioglyphe megastomus (Digenea, Omphalometridae) in Poland Zoodiversity 18s rrna gammarus pulex pomeranian region metacercaria |
| title | Molecular Characterization of the First Reported Neoplagioglyphe megastomus (Digenea, Omphalometridae) in Poland |
| title_full | Molecular Characterization of the First Reported Neoplagioglyphe megastomus (Digenea, Omphalometridae) in Poland |
| title_fullStr | Molecular Characterization of the First Reported Neoplagioglyphe megastomus (Digenea, Omphalometridae) in Poland |
| title_full_unstemmed | Molecular Characterization of the First Reported Neoplagioglyphe megastomus (Digenea, Omphalometridae) in Poland |
| title_short | Molecular Characterization of the First Reported Neoplagioglyphe megastomus (Digenea, Omphalometridae) in Poland |
| title_sort | molecular characterization of the first reported neoplagioglyphe megastomus digenea omphalometridae in poland |
| topic | 18s rrna gammarus pulex pomeranian region metacercaria |
| url | http://ojs.akademperiodyka.org.ua/index.php/Zoodiversity/article/view/302 |
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