De novo transcriptome profiling unveils the regulation of phenylpropanoid biosynthesis in unripe Piper nigrum berries
Abstract Background Black pepper (Piper nigrum L.) is rich in bioactive compounds that make it an imperative constituent in traditional medicines. Although the unripe fruits have long been used in different Ayurvedic formulations, the mechanism of gene regulation resulting in the production of the b...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-10-01
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| Series: | BMC Plant Biology |
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| Online Access: | https://doi.org/10.1186/s12870-022-03878-1 |
| _version_ | 1811257110678208512 |
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| author | Sweda Sreekumar Kattupalli Divya Nisha Joy E. V. Soniya |
| author_facet | Sweda Sreekumar Kattupalli Divya Nisha Joy E. V. Soniya |
| author_sort | Sweda Sreekumar |
| collection | DOAJ |
| description | Abstract Background Black pepper (Piper nigrum L.) is rich in bioactive compounds that make it an imperative constituent in traditional medicines. Although the unripe fruits have long been used in different Ayurvedic formulations, the mechanism of gene regulation resulting in the production of the bioactive compounds in black pepper is not much investigated. Exploring the regulatory factors favouring the production of bioactive compounds ultimately help to accumulate the medicinally important content of black pepper. The factors that enhance the biosynthesis of these compounds could be potential candidates for metabolic engineering strategies to obtain a high level production of significant biomolecules. Results Being a non-model plant, de novo sequencing technology was used to unravel comprehensive information about the genes and transcription factors that are expressed in mature unripe green berries of P. nigrum from which commercially available black pepper is prepared. In this study, the key gene regulations involved in the synthesis of bioactive principles in black pepper was brought out with a focus on the highly expressed phenylpropanoid pathway genes. Quantitative real-time PCR analysis of critical genes and transcription factors in the different developmental stages from bud to the mature green berries provides important information useful for choosing the developmental stage that would be best for the production of a particular bioactive compound. Comparison with a previous study has also been included to understand the relative position of the results obtained from this study. Conclusions The current study uncovered significant information regarding the gene expression and regulation responsible for the bioactivity of black pepper. The key transcription factors and enzymes analyzed in this study are promising targets for achieving a high level production of significant biomolecules through metabolic engineering. |
| first_indexed | 2024-04-12T17:52:21Z |
| format | Article |
| id | doaj.art-f85ef597974b48648f0dca75ef8eca2b |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-04-12T17:52:21Z |
| publishDate | 2022-10-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-f85ef597974b48648f0dca75ef8eca2b2022-12-22T03:22:29ZengBMCBMC Plant Biology1471-22292022-10-0122111410.1186/s12870-022-03878-1De novo transcriptome profiling unveils the regulation of phenylpropanoid biosynthesis in unripe Piper nigrum berriesSweda Sreekumar0Kattupalli Divya1Nisha Joy2E. V. Soniya3Transdisciplinary Biology, Rajiv Gandhi Centre for Biotechnology (RGCB)Transdisciplinary Biology, Rajiv Gandhi Centre for Biotechnology (RGCB)Transdisciplinary Biology, Rajiv Gandhi Centre for Biotechnology (RGCB)Transdisciplinary Biology, Rajiv Gandhi Centre for Biotechnology (RGCB)Abstract Background Black pepper (Piper nigrum L.) is rich in bioactive compounds that make it an imperative constituent in traditional medicines. Although the unripe fruits have long been used in different Ayurvedic formulations, the mechanism of gene regulation resulting in the production of the bioactive compounds in black pepper is not much investigated. Exploring the regulatory factors favouring the production of bioactive compounds ultimately help to accumulate the medicinally important content of black pepper. The factors that enhance the biosynthesis of these compounds could be potential candidates for metabolic engineering strategies to obtain a high level production of significant biomolecules. Results Being a non-model plant, de novo sequencing technology was used to unravel comprehensive information about the genes and transcription factors that are expressed in mature unripe green berries of P. nigrum from which commercially available black pepper is prepared. In this study, the key gene regulations involved in the synthesis of bioactive principles in black pepper was brought out with a focus on the highly expressed phenylpropanoid pathway genes. Quantitative real-time PCR analysis of critical genes and transcription factors in the different developmental stages from bud to the mature green berries provides important information useful for choosing the developmental stage that would be best for the production of a particular bioactive compound. Comparison with a previous study has also been included to understand the relative position of the results obtained from this study. Conclusions The current study uncovered significant information regarding the gene expression and regulation responsible for the bioactivity of black pepper. The key transcription factors and enzymes analyzed in this study are promising targets for achieving a high level production of significant biomolecules through metabolic engineering.https://doi.org/10.1186/s12870-022-03878-1Black pepperFruitsRNA-SeqDevelopmental stagesSecondary metabolite |
| spellingShingle | Sweda Sreekumar Kattupalli Divya Nisha Joy E. V. Soniya De novo transcriptome profiling unveils the regulation of phenylpropanoid biosynthesis in unripe Piper nigrum berries BMC Plant Biology Black pepper Fruits RNA-Seq Developmental stages Secondary metabolite |
| title | De novo transcriptome profiling unveils the regulation of phenylpropanoid biosynthesis in unripe Piper nigrum berries |
| title_full | De novo transcriptome profiling unveils the regulation of phenylpropanoid biosynthesis in unripe Piper nigrum berries |
| title_fullStr | De novo transcriptome profiling unveils the regulation of phenylpropanoid biosynthesis in unripe Piper nigrum berries |
| title_full_unstemmed | De novo transcriptome profiling unveils the regulation of phenylpropanoid biosynthesis in unripe Piper nigrum berries |
| title_short | De novo transcriptome profiling unveils the regulation of phenylpropanoid biosynthesis in unripe Piper nigrum berries |
| title_sort | de novo transcriptome profiling unveils the regulation of phenylpropanoid biosynthesis in unripe piper nigrum berries |
| topic | Black pepper Fruits RNA-Seq Developmental stages Secondary metabolite |
| url | https://doi.org/10.1186/s12870-022-03878-1 |
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