Comparison of Glucose Administration Routes to Determine Glucose Tolerance in Old and Young Mice
Background: It has been observed that old mice have a better tolerance to glucose than young after an intraperitoneal injection of glucose. We hypothesized that an intraperitoneal injection of glucose overestimates glucose tolerance in old mice. Methods: We assessed differences in glucose tolera...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Lithuanian Sports University
2022-11-01
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| Series: | Baltic Journal of Sport and Health Sciences |
| Online Access: | https://journals.lsu.lt/baltic-journal-of-sport-health/article/view/1274 |
| Summary: | Background: It has been observed that old mice have a better tolerance to glucose than young after an intraperitoneal injection of glucose. We hypothesized that an intraperitoneal injection of glucose overestimates glucose tolerance in old mice.
Methods: We assessed differences in glucose tolerance outcomes in 2- (young, n=23) and 23- to 27-month-old (old, n=23) male C57BL/6J mice after an intraperitoneal (IP) or intravenous (IV) injection, or oral gavage (OG) of glucose.
Results: The area under the curve (AUC) of the changes of blood glucose concentration over 2 hours after glucose administration was lower in old than young animals (p<0.001). The AUC was higher after IV than either IP or OG (p<0.001). However, normalized to peak glucose concentration, the time course was similar in young and old animals, and was – except for an earlier peak in IV than OG (p=0.013) – independent of route of administration.
Conclusion: We suggest that 1) to determine glucose tolerance the time course of changes in glucose concentration rather than the AUC, which is significantly affected by excursion magnitude, is preferable; 2) glucose tolerance can be measured equally well with IP, IV and OG; 3) there is no significant age-related difference in glucose tolerance in mice.
Keywords: glucose tolerance test, glucose uptake, aging, glucose intolerance, mice, diabetes.
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| ISSN: | 2351-6496 2538-8347 |