Comparison of Glucose Administration Routes to Determine Glucose Tolerance in Old and Young Mice
Background: It has been observed that old mice have a better tolerance to glucose than young after an intraperitoneal injection of glucose. We hypothesized that an intraperitoneal injection of glucose overestimates glucose tolerance in old mice. Methods: We assessed differences in glucose tolera...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Lithuanian Sports University
2022-11-01
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| Series: | Baltic Journal of Sport and Health Sciences |
| Online Access: | https://journals.lsu.lt/baltic-journal-of-sport-health/article/view/1274 |
| _version_ | 1811217675103240192 |
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| author | Anandini Swaminathan Indrė Libnickienė Tomas Venckūnas Hans Degens |
| author_facet | Anandini Swaminathan Indrė Libnickienė Tomas Venckūnas Hans Degens |
| author_sort | Anandini Swaminathan |
| collection | DOAJ |
| description |
Background: It has been observed that old mice have a better tolerance to glucose than young after an intraperitoneal injection of glucose. We hypothesized that an intraperitoneal injection of glucose overestimates glucose tolerance in old mice.
Methods: We assessed differences in glucose tolerance outcomes in 2- (young, n=23) and 23- to 27-month-old (old, n=23) male C57BL/6J mice after an intraperitoneal (IP) or intravenous (IV) injection, or oral gavage (OG) of glucose.
Results: The area under the curve (AUC) of the changes of blood glucose concentration over 2 hours after glucose administration was lower in old than young animals (p<0.001). The AUC was higher after IV than either IP or OG (p<0.001). However, normalized to peak glucose concentration, the time course was similar in young and old animals, and was – except for an earlier peak in IV than OG (p=0.013) – independent of route of administration.
Conclusion: We suggest that 1) to determine glucose tolerance the time course of changes in glucose concentration rather than the AUC, which is significantly affected by excursion magnitude, is preferable; 2) glucose tolerance can be measured equally well with IP, IV and OG; 3) there is no significant age-related difference in glucose tolerance in mice.
Keywords: glucose tolerance test, glucose uptake, aging, glucose intolerance, mice, diabetes.
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| first_indexed | 2024-04-12T06:58:46Z |
| format | Article |
| id | doaj.art-f85ff87794dd476582fba4b67bcb804e |
| institution | Directory Open Access Journal |
| issn | 2351-6496 2538-8347 |
| language | English |
| last_indexed | 2024-04-12T06:58:46Z |
| publishDate | 2022-11-01 |
| publisher | Lithuanian Sports University |
| record_format | Article |
| series | Baltic Journal of Sport and Health Sciences |
| spelling | doaj.art-f85ff87794dd476582fba4b67bcb804e2022-12-22T03:43:05ZengLithuanian Sports UniversityBaltic Journal of Sport and Health Sciences2351-64962538-83472022-11-01312610.33607/bjshs.v3i126.1274Comparison of Glucose Administration Routes to Determine Glucose Tolerance in Old and Young MiceAnandini Swaminathan0Indrė Libnickienė1Tomas Venckūnas2Hans Degens3Institute of Sport Science and Innovations, Lithuanian Sports University, KaunasInstitute of Sport Science and Innovations, Lithuanian Sports University, KaunasInstitute of Sport Science and Innovations, Lithuanian Sports University, KaunasDepartment of Life Sciences, Research Centre for Musculoskeletal Science and Sports Medicine, Manchester Metropolitan University, Manchester; Institute of Sport Science and Innovations, Lithuanian Sports University, Kaunas Background: It has been observed that old mice have a better tolerance to glucose than young after an intraperitoneal injection of glucose. We hypothesized that an intraperitoneal injection of glucose overestimates glucose tolerance in old mice. Methods: We assessed differences in glucose tolerance outcomes in 2- (young, n=23) and 23- to 27-month-old (old, n=23) male C57BL/6J mice after an intraperitoneal (IP) or intravenous (IV) injection, or oral gavage (OG) of glucose. Results: The area under the curve (AUC) of the changes of blood glucose concentration over 2 hours after glucose administration was lower in old than young animals (p<0.001). The AUC was higher after IV than either IP or OG (p<0.001). However, normalized to peak glucose concentration, the time course was similar in young and old animals, and was – except for an earlier peak in IV than OG (p=0.013) – independent of route of administration. Conclusion: We suggest that 1) to determine glucose tolerance the time course of changes in glucose concentration rather than the AUC, which is significantly affected by excursion magnitude, is preferable; 2) glucose tolerance can be measured equally well with IP, IV and OG; 3) there is no significant age-related difference in glucose tolerance in mice. Keywords: glucose tolerance test, glucose uptake, aging, glucose intolerance, mice, diabetes. https://journals.lsu.lt/baltic-journal-of-sport-health/article/view/1274 |
| spellingShingle | Anandini Swaminathan Indrė Libnickienė Tomas Venckūnas Hans Degens Comparison of Glucose Administration Routes to Determine Glucose Tolerance in Old and Young Mice Baltic Journal of Sport and Health Sciences |
| title | Comparison of Glucose Administration Routes to Determine Glucose Tolerance in Old and Young Mice |
| title_full | Comparison of Glucose Administration Routes to Determine Glucose Tolerance in Old and Young Mice |
| title_fullStr | Comparison of Glucose Administration Routes to Determine Glucose Tolerance in Old and Young Mice |
| title_full_unstemmed | Comparison of Glucose Administration Routes to Determine Glucose Tolerance in Old and Young Mice |
| title_short | Comparison of Glucose Administration Routes to Determine Glucose Tolerance in Old and Young Mice |
| title_sort | comparison of glucose administration routes to determine glucose tolerance in old and young mice |
| url | https://journals.lsu.lt/baltic-journal-of-sport-health/article/view/1274 |
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