Pterostilbene attenuates intestinal epithelial barrier loss induced by high loading intensity of exercise
Mounting evidence suggested that high loading intensity of exercise might be detrimental to human health, especially the gastrointestinal tract. Pterostilbene (PTE), derived from grapes and blueberries, might reach a high concentration of intestinal contents. Our study aimed to evaluate PTE’s abilit...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-08-01
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| Series: | Frontiers in Nutrition |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2022.965180/full |
| _version_ | 1818172801179189248 |
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| author | Lidong Zhang Guoliang Tian Li Huang Min Zhou Jundong Zhu Long Yi Mantian Mi |
| author_facet | Lidong Zhang Guoliang Tian Li Huang Min Zhou Jundong Zhu Long Yi Mantian Mi |
| author_sort | Lidong Zhang |
| collection | DOAJ |
| description | Mounting evidence suggested that high loading intensity of exercise might be detrimental to human health, especially the gastrointestinal tract. Pterostilbene (PTE), derived from grapes and blueberries, might reach a high concentration of intestinal contents. Our study aimed to evaluate PTE’s ability to prevent the loss of intestinal epithelial barrier in high loading intensity of exercise. The exercise model was established by the forced running of mice. An effective HPLC-UV method was developed to quantify PTE concentration in intestinal content. The mRNA changes were detected by quantitative polymerase chain reaction (qPCR). The structure of intestinal flora was analyzed by 16S rRNA sequencing. The PTE (100 mg/kg/d) could significantly attenuate exercise-induced intestinal epithelial barrier loss. Moreover, the HPLC-UV assay showed that the PTE concentration of intestinal content could last 12 h. Furthermore, the exercise increased the abundance of Alistipes, which was related to lipopolysaccharide (LPS) production but could not be reversed by PTE intervention. Besides, cell experiments showed that PTE could promote the expression of intestinal epithelial tight junction (TJ) molecules in vitro. In conclusion, PTE has a significant interest in preventing exercise-induced intestinal damage. |
| first_indexed | 2024-12-11T19:18:23Z |
| format | Article |
| id | doaj.art-f860c74c7970457cb6af96fd703142ef |
| institution | Directory Open Access Journal |
| issn | 2296-861X |
| language | English |
| last_indexed | 2024-12-11T19:18:23Z |
| publishDate | 2022-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Nutrition |
| spelling | doaj.art-f860c74c7970457cb6af96fd703142ef2022-12-22T00:53:36ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2022-08-01910.3389/fnut.2022.965180965180Pterostilbene attenuates intestinal epithelial barrier loss induced by high loading intensity of exerciseLidong ZhangGuoliang TianLi HuangMin ZhouJundong ZhuLong YiMantian MiMounting evidence suggested that high loading intensity of exercise might be detrimental to human health, especially the gastrointestinal tract. Pterostilbene (PTE), derived from grapes and blueberries, might reach a high concentration of intestinal contents. Our study aimed to evaluate PTE’s ability to prevent the loss of intestinal epithelial barrier in high loading intensity of exercise. The exercise model was established by the forced running of mice. An effective HPLC-UV method was developed to quantify PTE concentration in intestinal content. The mRNA changes were detected by quantitative polymerase chain reaction (qPCR). The structure of intestinal flora was analyzed by 16S rRNA sequencing. The PTE (100 mg/kg/d) could significantly attenuate exercise-induced intestinal epithelial barrier loss. Moreover, the HPLC-UV assay showed that the PTE concentration of intestinal content could last 12 h. Furthermore, the exercise increased the abundance of Alistipes, which was related to lipopolysaccharide (LPS) production but could not be reversed by PTE intervention. Besides, cell experiments showed that PTE could promote the expression of intestinal epithelial tight junction (TJ) molecules in vitro. In conclusion, PTE has a significant interest in preventing exercise-induced intestinal damage.https://www.frontiersin.org/articles/10.3389/fnut.2022.965180/fullexercisepterostilbene (PTE)lipopolysaccharide (LPS)intestinal barrierintestinal flora |
| spellingShingle | Lidong Zhang Guoliang Tian Li Huang Min Zhou Jundong Zhu Long Yi Mantian Mi Pterostilbene attenuates intestinal epithelial barrier loss induced by high loading intensity of exercise Frontiers in Nutrition exercise pterostilbene (PTE) lipopolysaccharide (LPS) intestinal barrier intestinal flora |
| title | Pterostilbene attenuates intestinal epithelial barrier loss induced by high loading intensity of exercise |
| title_full | Pterostilbene attenuates intestinal epithelial barrier loss induced by high loading intensity of exercise |
| title_fullStr | Pterostilbene attenuates intestinal epithelial barrier loss induced by high loading intensity of exercise |
| title_full_unstemmed | Pterostilbene attenuates intestinal epithelial barrier loss induced by high loading intensity of exercise |
| title_short | Pterostilbene attenuates intestinal epithelial barrier loss induced by high loading intensity of exercise |
| title_sort | pterostilbene attenuates intestinal epithelial barrier loss induced by high loading intensity of exercise |
| topic | exercise pterostilbene (PTE) lipopolysaccharide (LPS) intestinal barrier intestinal flora |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2022.965180/full |
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