Antiulcer effects of Zataria multiflora Boiss. on indomethacin-induced gastric ulcer in rats
Objective: Zataria multiflora has been reported to have several medicinal properties including antioxidant, antibacterial, antispasmodic, and expectorant activities. This study aimed to investigate the effect of Z. multiflora hydro-alcoholic extract (ZMHE) on peptic ulcer caused by indomethacin in r...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Mashhad University of Medical Sciences
2018-09-01
|
| Series: | Avicenna Journal of Phytomedicine |
| Subjects: | |
| Online Access: | http://ajp.mums.ac.ir/article_10617_e580a089457dc067f3b1223ba3d60efd.pdf |
| _version_ | 1818874730701127680 |
|---|---|
| author | Mohsen Minaiyan Sayed-Ebrahim Sajjadi Kamran Amini-Dehkordi |
| author_facet | Mohsen Minaiyan Sayed-Ebrahim Sajjadi Kamran Amini-Dehkordi |
| author_sort | Mohsen Minaiyan |
| collection | DOAJ |
| description | Objective: Zataria multiflora has been reported to have several medicinal properties including antioxidant, antibacterial, antispasmodic, and expectorant activities. This study aimed to investigate the effect of Z. multiflora hydro-alcoholic extract (ZMHE) on peptic ulcer caused by indomethacin in rats. Materials and Methods: ZMHE was prepared by maceration, condensed by rotary evaporator and dried by a freeze-drier. In this study, 72 male Wistar rats were randomly divided into 12 groups, six in each including: normal rats, control rats, ranitidine-treated, and animals that were treated with ZMHE (100, 200, 400 mg/kg). Parenteral and oral treatments were done 1 and 2 hours, respectively before gastric ulcer induction by indomethacin (25 mg/kg, i.p.). After 6 hours, animals were sacrificed, two sides of the stomach were ligated and gastric contents and tissues were investigated in terms of pH and biochemical and histologic parameters, respectively. Results: ZMHE (at all doses) considerably decreased the ulcer area and ulcer severity in comparison to control group after oral and parenteral administration. Oral administration of this extract increased thepH of stomach contents while diminished pepsin activity in a dose-dependent manner. Following parenteral treatment, a significant difference in pH of stomach content was observed only by ZMHE 400mg/kg in comparison to control group. The myeloperoxidase (MPO) enzyme activity decreased in groups that received the extract via both oral and parenteral routes. Conclusion: It might be concluded that ZMHE could protect against experimental gastric ulcer induced by indomethacin and this action is probably mediated via reducing acid secretion and pepsin activity besides enhancing tissue antioxidant capacity |
| first_indexed | 2024-12-19T13:15:15Z |
| format | Article |
| id | doaj.art-f860d25df8454ee0a8fa894a3c0a4f21 |
| institution | Directory Open Access Journal |
| issn | 2228-7930 2228-7949 |
| language | English |
| last_indexed | 2024-12-19T13:15:15Z |
| publishDate | 2018-09-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Avicenna Journal of Phytomedicine |
| spelling | doaj.art-f860d25df8454ee0a8fa894a3c0a4f212022-12-21T20:19:50ZengMashhad University of Medical SciencesAvicenna Journal of Phytomedicine2228-79302228-79492018-09-018540841510.22038/ajp.2018.1061710617Antiulcer effects of Zataria multiflora Boiss. on indomethacin-induced gastric ulcer in ratsMohsen Minaiyan0Sayed-Ebrahim Sajjadi1Kamran Amini-Dehkordi2Department of Pharmacology and Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, IranDepartment of Pharmacognosy, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, IranSchool of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, IranObjective: Zataria multiflora has been reported to have several medicinal properties including antioxidant, antibacterial, antispasmodic, and expectorant activities. This study aimed to investigate the effect of Z. multiflora hydro-alcoholic extract (ZMHE) on peptic ulcer caused by indomethacin in rats. Materials and Methods: ZMHE was prepared by maceration, condensed by rotary evaporator and dried by a freeze-drier. In this study, 72 male Wistar rats were randomly divided into 12 groups, six in each including: normal rats, control rats, ranitidine-treated, and animals that were treated with ZMHE (100, 200, 400 mg/kg). Parenteral and oral treatments were done 1 and 2 hours, respectively before gastric ulcer induction by indomethacin (25 mg/kg, i.p.). After 6 hours, animals were sacrificed, two sides of the stomach were ligated and gastric contents and tissues were investigated in terms of pH and biochemical and histologic parameters, respectively. Results: ZMHE (at all doses) considerably decreased the ulcer area and ulcer severity in comparison to control group after oral and parenteral administration. Oral administration of this extract increased thepH of stomach contents while diminished pepsin activity in a dose-dependent manner. Following parenteral treatment, a significant difference in pH of stomach content was observed only by ZMHE 400mg/kg in comparison to control group. The myeloperoxidase (MPO) enzyme activity decreased in groups that received the extract via both oral and parenteral routes. Conclusion: It might be concluded that ZMHE could protect against experimental gastric ulcer induced by indomethacin and this action is probably mediated via reducing acid secretion and pepsin activity besides enhancing tissue antioxidant capacityhttp://ajp.mums.ac.ir/article_10617_e580a089457dc067f3b1223ba3d60efd.pdfZataria multifloraGastric ulcerGastric acidPepsinUlcer index |
| spellingShingle | Mohsen Minaiyan Sayed-Ebrahim Sajjadi Kamran Amini-Dehkordi Antiulcer effects of Zataria multiflora Boiss. on indomethacin-induced gastric ulcer in rats Avicenna Journal of Phytomedicine Zataria multiflora Gastric ulcer Gastric acid Pepsin Ulcer index |
| title | Antiulcer effects of Zataria multiflora Boiss. on indomethacin-induced gastric ulcer in rats |
| title_full | Antiulcer effects of Zataria multiflora Boiss. on indomethacin-induced gastric ulcer in rats |
| title_fullStr | Antiulcer effects of Zataria multiflora Boiss. on indomethacin-induced gastric ulcer in rats |
| title_full_unstemmed | Antiulcer effects of Zataria multiflora Boiss. on indomethacin-induced gastric ulcer in rats |
| title_short | Antiulcer effects of Zataria multiflora Boiss. on indomethacin-induced gastric ulcer in rats |
| title_sort | antiulcer effects of zataria multiflora boiss on indomethacin induced gastric ulcer in rats |
| topic | Zataria multiflora Gastric ulcer Gastric acid Pepsin Ulcer index |
| url | http://ajp.mums.ac.ir/article_10617_e580a089457dc067f3b1223ba3d60efd.pdf |
| work_keys_str_mv | AT mohsenminaiyan antiulcereffectsofzatariamultifloraboissonindomethacininducedgastriculcerinrats AT sayedebrahimsajjadi antiulcereffectsofzatariamultifloraboissonindomethacininducedgastriculcerinrats AT kamranaminidehkordi antiulcereffectsofzatariamultifloraboissonindomethacininducedgastriculcerinrats |