Protocol for whole-mount X-gal staining combined with tissue clearing in embryo and adult mouse using CUBIC

Protocol for whole-mount X-gal staining combined with tissue clearing in embryo and adult mouse using CUBIC

Summary: Here we describe an optimized protocol for X-gal staining of tissue clearing embryo and adult mouse using CUBIC. The activity of LacZ knock-in reflecting endogenous expression of genes of interest in the whole body can be visualized by X-gal staining. This protocol is suitable for examining...

書目詳細資料
Main Authors:	Haruko Watanabe-Takano, Moe Fukumoto, Shigetomo Fukuhara, Naoki Mochizuki
格式:	Article
語言:	English
出版:	Elsevier 2022-03-01
叢編:	STAR Protocols
主題:	Developmental biology Genetics Microbiology Model Organisms
在線閱讀:	http://www.sciencedirect.com/science/article/pii/S2666166722000077

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