A Maverick-like cluster in the genome of a pathogenic, moderately virulent strain of Gallibacterium anatis, ESV200, a transient biofilm producer
IntroductionGallibacterium anatis causes gallibacteriosis in birds. These bacteria produce biofilms and secrete several fimbrial appendages as tools to cause disease in animals. G. anatis strains contain up to three types of fimbriae. Complete genome sequencing is the strategy currently used to dete...
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Frontiers Media S.A.
2023-01-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1084766/full |
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| author | Patricia Sanchez-Alonso Elena Cobos-Justo Miguel Angel Avalos-Rangel Lucía López-Reyes Gloria Luz Paniagua-Contreras Felipe Vaca-Paniagua Felipe Vaca-Paniagua Estela Anastacio-Marcelino Ana Jaqueline López-Ochoa Victor M. Pérez Marquez Erasmo Negrete-Abascal Candelario Vázquez-Cruz |
| author_facet | Patricia Sanchez-Alonso Elena Cobos-Justo Miguel Angel Avalos-Rangel Lucía López-Reyes Gloria Luz Paniagua-Contreras Felipe Vaca-Paniagua Felipe Vaca-Paniagua Estela Anastacio-Marcelino Ana Jaqueline López-Ochoa Victor M. Pérez Marquez Erasmo Negrete-Abascal Candelario Vázquez-Cruz |
| author_sort | Patricia Sanchez-Alonso |
| collection | DOAJ |
| description | IntroductionGallibacterium anatis causes gallibacteriosis in birds. These bacteria produce biofilms and secrete several fimbrial appendages as tools to cause disease in animals. G. anatis strains contain up to three types of fimbriae. Complete genome sequencing is the strategy currently used to determine variations in the gene content of G. anatis, although today only the completely circularized genome of G. anatis UMN179 is available.MethodsThe appearance of growth of various strains of G. anatis in liquid culture medium was studied. Biofilm production and how the amount of biofilm was affected by DNase, Proteinase K, and Pronase E enzymes were analyzed. Fimbrial gene expression was performed by protein analysis and qRT-PCR. In an avian model, the pathogenesis generated by the strains G. anatis ESV200 and 12656-12 was investigated. Using bioinformatic tools, the complete genome of G. anatis ESV200 was comparatively studied to search for virulence factors that would help explain the pathogenic behavior of this strain.Results and DiscussionG. anatis ESV200 strain differs from the 12656-12 strain because it produces a biofilm at 20%. G. anatis ESV200 strain express fimbrial genes and produces biofilm but with a different structure than that observed for strain 12656-12. ESV200 and 12656-12 strains are pathogenic for chickens, although the latter is the most virulent. Here, we show that the complete genome of the ESV200 strain is similar to that of the UNM179 strain. However, these strains have evolved with many structural rearrangements; the most striking chromosomal arrangement is a Maverick-like element present in the ESV200 strain. |
| first_indexed | 2024-04-10T20:14:03Z |
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| id | doaj.art-f86365100dd9479aab1ec05aacf7f56c |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-10T20:14:03Z |
| publishDate | 2023-01-01 |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-f86365100dd9479aab1ec05aacf7f56c2023-01-26T08:09:30ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-01-011410.3389/fmicb.2023.10847661084766A Maverick-like cluster in the genome of a pathogenic, moderately virulent strain of Gallibacterium anatis, ESV200, a transient biofilm producerPatricia Sanchez-Alonso0Elena Cobos-Justo1Miguel Angel Avalos-Rangel2Lucía López-Reyes3Gloria Luz Paniagua-Contreras4Felipe Vaca-Paniagua5Felipe Vaca-Paniagua6Estela Anastacio-Marcelino7Ana Jaqueline López-Ochoa8Victor M. Pérez Marquez9Erasmo Negrete-Abascal10Candelario Vázquez-Cruz11Centro de Investigaciones en Ciencias Microbiológicas, Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla, Puebla, MexicoCentro de Investigaciones en Ciencias Microbiológicas, Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla, Puebla, MexicoCentro de Investigaciones en Ciencias Microbiológicas, Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla, Puebla, MexicoCentro de Investigaciones en Ciencias Microbiológicas, Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla, Puebla, MexicoCarrera de Biología, Facultad de Estudios Superiores de Iztacala, UNAM, Los Reyes Iztacala, Estado de, México, MexicoCarrera de Biología, Facultad de Estudios Superiores de Iztacala, UNAM, Los Reyes Iztacala, Estado de, México, MexicoSubdirección de Investigación Basica, Instituto Nacional de Cancerología, CDMX, MéxicoCentro de Investigaciones en Ciencias Microbiológicas, Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla, Puebla, MexicoCentro de Investigaciones en Ciencias Microbiológicas, Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla, Puebla, MexicoDiagnóstico y Patobiología Aviar, Biotecnología Veterinaria S.A.-Biovetsa, BIOVETSA, Tehuacán, MexicoCarrera de Biología, Facultad de Estudios Superiores de Iztacala, UNAM, Los Reyes Iztacala, Estado de, México, MexicoCentro de Investigaciones en Ciencias Microbiológicas, Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla, Puebla, MexicoIntroductionGallibacterium anatis causes gallibacteriosis in birds. These bacteria produce biofilms and secrete several fimbrial appendages as tools to cause disease in animals. G. anatis strains contain up to three types of fimbriae. Complete genome sequencing is the strategy currently used to determine variations in the gene content of G. anatis, although today only the completely circularized genome of G. anatis UMN179 is available.MethodsThe appearance of growth of various strains of G. anatis in liquid culture medium was studied. Biofilm production and how the amount of biofilm was affected by DNase, Proteinase K, and Pronase E enzymes were analyzed. Fimbrial gene expression was performed by protein analysis and qRT-PCR. In an avian model, the pathogenesis generated by the strains G. anatis ESV200 and 12656-12 was investigated. Using bioinformatic tools, the complete genome of G. anatis ESV200 was comparatively studied to search for virulence factors that would help explain the pathogenic behavior of this strain.Results and DiscussionG. anatis ESV200 strain differs from the 12656-12 strain because it produces a biofilm at 20%. G. anatis ESV200 strain express fimbrial genes and produces biofilm but with a different structure than that observed for strain 12656-12. ESV200 and 12656-12 strains are pathogenic for chickens, although the latter is the most virulent. Here, we show that the complete genome of the ESV200 strain is similar to that of the UNM179 strain. However, these strains have evolved with many structural rearrangements; the most striking chromosomal arrangement is a Maverick-like element present in the ESV200 strain.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1084766/fullgenomefimbriaebiofilmpathogenesisavian diseasesGallibacterium anatis |
| spellingShingle | Patricia Sanchez-Alonso Elena Cobos-Justo Miguel Angel Avalos-Rangel Lucía López-Reyes Gloria Luz Paniagua-Contreras Felipe Vaca-Paniagua Felipe Vaca-Paniagua Estela Anastacio-Marcelino Ana Jaqueline López-Ochoa Victor M. Pérez Marquez Erasmo Negrete-Abascal Candelario Vázquez-Cruz A Maverick-like cluster in the genome of a pathogenic, moderately virulent strain of Gallibacterium anatis, ESV200, a transient biofilm producer Frontiers in Microbiology genome fimbriae biofilm pathogenesis avian diseases Gallibacterium anatis |
| title | A Maverick-like cluster in the genome of a pathogenic, moderately virulent strain of Gallibacterium anatis, ESV200, a transient biofilm producer |
| title_full | A Maverick-like cluster in the genome of a pathogenic, moderately virulent strain of Gallibacterium anatis, ESV200, a transient biofilm producer |
| title_fullStr | A Maverick-like cluster in the genome of a pathogenic, moderately virulent strain of Gallibacterium anatis, ESV200, a transient biofilm producer |
| title_full_unstemmed | A Maverick-like cluster in the genome of a pathogenic, moderately virulent strain of Gallibacterium anatis, ESV200, a transient biofilm producer |
| title_short | A Maverick-like cluster in the genome of a pathogenic, moderately virulent strain of Gallibacterium anatis, ESV200, a transient biofilm producer |
| title_sort | maverick like cluster in the genome of a pathogenic moderately virulent strain of gallibacterium anatis esv200 a transient biofilm producer |
| topic | genome fimbriae biofilm pathogenesis avian diseases Gallibacterium anatis |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1084766/full |
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