Karyology Study of Ten Trifolium Species in Fars Province

Chromosome information is an important key for taxonomy, phylogeny and genetic in Trifolium plants. Thus, ten Trifolium species namely T. grandiflorum, T. resupinatum, T. dasyurum, T. campestre, T. tomentosum, T. hirtum, T. scabrum, T. lappaceum, T. stellatum, and T. repens were collected from their...

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Main Authors: Mehrnaz Riasat, Sara Sadeghian
Format: Article
Language:English
Published: University of Mazandaran 2019-07-01
Series:Journal of Genetic Resources
Subjects:
Online Access:http://sc.journals.umz.ac.ir/article_2419_70e647f0eb8f8b632913fdb167ac31ef.pdf
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author Mehrnaz Riasat
Sara Sadeghian
author_facet Mehrnaz Riasat
Sara Sadeghian
author_sort Mehrnaz Riasat
collection DOAJ
description Chromosome information is an important key for taxonomy, phylogeny and genetic in Trifolium plants. Thus, ten Trifolium species namely T. grandiflorum, T. resupinatum, T. dasyurum, T. campestre, T. tomentosum, T. hirtum, T. scabrum, T. lappaceum, T. stellatum, and T. repens were collected from their habitats in Fars province based on their morphological characteristics to investigate their karyotypes. To analyze the karyology of samples, the fresh grown root tips were used. Then α-bromonaphtaline, formaldehyde and chromium trioxide (1:1), 1N NaOH and hematoxylin were used for pre-treatment, fixative, hydrolyzer and chromosome staining agent, respectively. We found the three usual basic chromosome numbers in the genus as x=5, x=7 and x=8. Nine species were diploid and T. repense was only tetraploid species (2n=4x=32). The detailed correlation coefficient was estimated for all paired combinations of the karyotypic characteristics to investigate their inter-relationships. Duncan’s test applied to the chromosome morphometric traits showed a highly significant difference among all examined species belongs to different sections.
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spelling doaj.art-f8636ef6c848474c9246d7887f7c370c2022-12-21T18:38:52ZengUniversity of MazandaranJournal of Genetic Resources2423-42572588-25892019-07-015213614210.22080/jgr.2019.24192419Karyology Study of Ten Trifolium Species in Fars ProvinceMehrnaz Riasat0Sara Sadeghian1Research Division of Natural Resources Department, Fars Agricultural and Natural Resources Research and Education Centre, AREEO, ShirazResearch Division of Natural Resources Department, Fars Agricultural and Natural Resources Research and Education Centre, AREEO, Shiraz,Chromosome information is an important key for taxonomy, phylogeny and genetic in Trifolium plants. Thus, ten Trifolium species namely T. grandiflorum, T. resupinatum, T. dasyurum, T. campestre, T. tomentosum, T. hirtum, T. scabrum, T. lappaceum, T. stellatum, and T. repens were collected from their habitats in Fars province based on their morphological characteristics to investigate their karyotypes. To analyze the karyology of samples, the fresh grown root tips were used. Then α-bromonaphtaline, formaldehyde and chromium trioxide (1:1), 1N NaOH and hematoxylin were used for pre-treatment, fixative, hydrolyzer and chromosome staining agent, respectively. We found the three usual basic chromosome numbers in the genus as x=5, x=7 and x=8. Nine species were diploid and T. repense was only tetraploid species (2n=4x=32). The detailed correlation coefficient was estimated for all paired combinations of the karyotypic characteristics to investigate their inter-relationships. Duncan’s test applied to the chromosome morphometric traits showed a highly significant difference among all examined species belongs to different sections.http://sc.journals.umz.ac.ir/article_2419_70e647f0eb8f8b632913fdb167ac31ef.pdfchromosomekaryologyiranpapilionaceaetrifolium
spellingShingle Mehrnaz Riasat
Sara Sadeghian
Karyology Study of Ten Trifolium Species in Fars Province
Journal of Genetic Resources
chromosome
karyology
iran
papilionaceae
trifolium
title Karyology Study of Ten Trifolium Species in Fars Province
title_full Karyology Study of Ten Trifolium Species in Fars Province
title_fullStr Karyology Study of Ten Trifolium Species in Fars Province
title_full_unstemmed Karyology Study of Ten Trifolium Species in Fars Province
title_short Karyology Study of Ten Trifolium Species in Fars Province
title_sort karyology study of ten trifolium species in fars province
topic chromosome
karyology
iran
papilionaceae
trifolium
url http://sc.journals.umz.ac.ir/article_2419_70e647f0eb8f8b632913fdb167ac31ef.pdf
work_keys_str_mv AT mehrnazriasat karyologystudyoftentrifoliumspeciesinfarsprovince
AT sarasadeghian karyologystudyoftentrifoliumspeciesinfarsprovince