Promoter of Vegetable Soybean <i>GmTIP1;6</i> Responds to Diverse Abiotic Stresses and Hormone Signals in Transgenic <i>Arabidopsis</i>
Tonoplast intrinsic proteins (TIPs), a sub-family of aquaporins (AQPs), are known to play important roles in plant abiotic stress responses. However, evidence for the promoters of TIPs involvement in abiotic stress processes remains scarce. In this study, the promoter of the vegetable soybean <i&...
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MDPI AG
2022-10-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/20/12684 |
| _version_ | 1797472650889527296 |
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| author | Zhijuan Feng Na Liu Guwen Zhang Yuanpeng Bu Bin Wang Yaming Gong |
| author_facet | Zhijuan Feng Na Liu Guwen Zhang Yuanpeng Bu Bin Wang Yaming Gong |
| author_sort | Zhijuan Feng |
| collection | DOAJ |
| description | Tonoplast intrinsic proteins (TIPs), a sub-family of aquaporins (AQPs), are known to play important roles in plant abiotic stress responses. However, evidence for the promoters of TIPs involvement in abiotic stress processes remains scarce. In this study, the promoter of the vegetable soybean <i>GmTIP1;6</i> gene, which had the highest similarity to TIP1-type AQPs from other plants, was cloned. Expression pattern analyses indicated that the <i>GmTIP1;6</i> gene was dramatically induced by drought, salt, abscisic acid (ABA), and methyl jasmonate (MeJA) stimuli. Promoter analyses revealed that the <i>GmTIP1;6</i> promoter contained drought, ABA, and MeJA <i>cis</i>-acting elements. Histochemical staining of the <i>GmTIP1;6</i> promoter in transgenic <i>Arabidopsis</i> corroborated that it was strongly expressed in the vascular bundles of leaves, stems, and roots. Beta-glucuronidase (GUS) activity assays showed that the activities of the <i>GmTIP1;6</i> promoter were enhanced by different concentrations of polyethylene glycol 6000 (PEG 6000), NaCl, ABA, and MEJA treatments. Integrating these results revealed that the <i>GmTIP1;6</i> promoter could be applied for improving the tolerance to abiotic stresses of the transgenic plants by promoting the expression of vegetable soybean <i>AQPs</i>. |
| first_indexed | 2024-03-09T20:05:23Z |
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| id | doaj.art-f864dc1db8404b6589c9b6b477877c74 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T20:05:23Z |
| publishDate | 2022-10-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-f864dc1db8404b6589c9b6b477877c742023-11-24T00:35:21ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-10-0123201268410.3390/ijms232012684Promoter of Vegetable Soybean <i>GmTIP1;6</i> Responds to Diverse Abiotic Stresses and Hormone Signals in Transgenic <i>Arabidopsis</i>Zhijuan Feng0Na Liu1Guwen Zhang2Yuanpeng Bu3Bin Wang4Yaming Gong5Institute of Vegetables, State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Key Laboratory of Vegetable Legumes Germplasm Enhancement and Molecular Breeding in Southern China of Ministry of Agriculture and Rural Affairs, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaInstitute of Vegetables, State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Key Laboratory of Vegetable Legumes Germplasm Enhancement and Molecular Breeding in Southern China of Ministry of Agriculture and Rural Affairs, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaInstitute of Vegetables, State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Key Laboratory of Vegetable Legumes Germplasm Enhancement and Molecular Breeding in Southern China of Ministry of Agriculture and Rural Affairs, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaInstitute of Vegetables, State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Key Laboratory of Vegetable Legumes Germplasm Enhancement and Molecular Breeding in Southern China of Ministry of Agriculture and Rural Affairs, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaInstitute of Vegetables, State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Key Laboratory of Vegetable Legumes Germplasm Enhancement and Molecular Breeding in Southern China of Ministry of Agriculture and Rural Affairs, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaInstitute of Vegetables, State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Key Laboratory of Vegetable Legumes Germplasm Enhancement and Molecular Breeding in Southern China of Ministry of Agriculture and Rural Affairs, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaTonoplast intrinsic proteins (TIPs), a sub-family of aquaporins (AQPs), are known to play important roles in plant abiotic stress responses. However, evidence for the promoters of TIPs involvement in abiotic stress processes remains scarce. In this study, the promoter of the vegetable soybean <i>GmTIP1;6</i> gene, which had the highest similarity to TIP1-type AQPs from other plants, was cloned. Expression pattern analyses indicated that the <i>GmTIP1;6</i> gene was dramatically induced by drought, salt, abscisic acid (ABA), and methyl jasmonate (MeJA) stimuli. Promoter analyses revealed that the <i>GmTIP1;6</i> promoter contained drought, ABA, and MeJA <i>cis</i>-acting elements. Histochemical staining of the <i>GmTIP1;6</i> promoter in transgenic <i>Arabidopsis</i> corroborated that it was strongly expressed in the vascular bundles of leaves, stems, and roots. Beta-glucuronidase (GUS) activity assays showed that the activities of the <i>GmTIP1;6</i> promoter were enhanced by different concentrations of polyethylene glycol 6000 (PEG 6000), NaCl, ABA, and MEJA treatments. Integrating these results revealed that the <i>GmTIP1;6</i> promoter could be applied for improving the tolerance to abiotic stresses of the transgenic plants by promoting the expression of vegetable soybean <i>AQPs</i>.https://www.mdpi.com/1422-0067/23/20/12684vegetable soybeanaquaporinpromoterGUStransgenic <i>Arabidopsis</i>abiotic stresses |
| spellingShingle | Zhijuan Feng Na Liu Guwen Zhang Yuanpeng Bu Bin Wang Yaming Gong Promoter of Vegetable Soybean <i>GmTIP1;6</i> Responds to Diverse Abiotic Stresses and Hormone Signals in Transgenic <i>Arabidopsis</i> International Journal of Molecular Sciences vegetable soybean aquaporin promoter GUS transgenic <i>Arabidopsis</i> abiotic stresses |
| title | Promoter of Vegetable Soybean <i>GmTIP1;6</i> Responds to Diverse Abiotic Stresses and Hormone Signals in Transgenic <i>Arabidopsis</i> |
| title_full | Promoter of Vegetable Soybean <i>GmTIP1;6</i> Responds to Diverse Abiotic Stresses and Hormone Signals in Transgenic <i>Arabidopsis</i> |
| title_fullStr | Promoter of Vegetable Soybean <i>GmTIP1;6</i> Responds to Diverse Abiotic Stresses and Hormone Signals in Transgenic <i>Arabidopsis</i> |
| title_full_unstemmed | Promoter of Vegetable Soybean <i>GmTIP1;6</i> Responds to Diverse Abiotic Stresses and Hormone Signals in Transgenic <i>Arabidopsis</i> |
| title_short | Promoter of Vegetable Soybean <i>GmTIP1;6</i> Responds to Diverse Abiotic Stresses and Hormone Signals in Transgenic <i>Arabidopsis</i> |
| title_sort | promoter of vegetable soybean i gmtip1 6 i responds to diverse abiotic stresses and hormone signals in transgenic i arabidopsis i |
| topic | vegetable soybean aquaporin promoter GUS transgenic <i>Arabidopsis</i> abiotic stresses |
| url | https://www.mdpi.com/1422-0067/23/20/12684 |
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