Development of an oral vaccine using recombinant viral haemorrhagic septicaemia virus glycoproteins produced in tobacco
The viral haemorrhagic septicaemia virus (VHSV) causes high mortality in many marine and freshwater fish species, resulting in heavy economic losses in fish farming. Previously, cholera toxin B subunit (CTB)-fused recombinant viral haemorrhagic septicaemia virus glycoproteins (rec-VHSV-GPs) have bee...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2019-10-01
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| Series: | Veterinární Medicína |
| Subjects: | |
| Online Access: | https://vetmed.agriculturejournals.cz/artkey/vet-201910-0005_development-of-an-oral-vaccine-using-recombinant-viral-haemorrhagic-septicaemia-virus-glycoproteins-produced-in.php |
| _version_ | 1828006532228317184 |
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| author | Tae Geum Kim Bong Jo Kang Sang Ik Park Tae Jung Kim |
| author_facet | Tae Geum Kim Bong Jo Kang Sang Ik Park Tae Jung Kim |
| author_sort | Tae Geum Kim |
| collection | DOAJ |
| description | The viral haemorrhagic septicaemia virus (VHSV) causes high mortality in many marine and freshwater fish species, resulting in heavy economic losses in fish farming. Previously, cholera toxin B subunit (CTB)-fused recombinant viral haemorrhagic septicaemia virus glycoproteins (rec-VHSV-GPs) have been successfully expressed in tobacco, Nicotiana benthamiana. Here, we evaluated the potential of rec-VHSV-GPs as an oral vaccine against a live viral challenge. After immunisation of mice and fish (olive flounder, Paralichthys olivaceous) with those antigenic proteins in a feed additive form, the antibody titres were increased statistically, especially in the primed groups (P < 0.0001) in both the mouse and fish. After the viral challenge under low water temperature culture conditions (below 18 °C), the immunised fish were protected successfully against the challenge, showing a significantly lower mortality rate (P < 0.05). This result suggests that this plant-based immunisation system could induce an effective immune response. It could be used as a candidate to develop an oral vaccine for fish. |
| first_indexed | 2024-04-10T07:59:11Z |
| format | Article |
| id | doaj.art-f865427e0534483e9e9926de235d6bde |
| institution | Directory Open Access Journal |
| issn | 0375-8427 1805-9392 |
| language | English |
| last_indexed | 2024-04-10T07:59:11Z |
| publishDate | 2019-10-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Veterinární Medicína |
| spelling | doaj.art-f865427e0534483e9e9926de235d6bde2023-02-23T03:50:53ZengCzech Academy of Agricultural SciencesVeterinární Medicína0375-84271805-93922019-10-01641045646110.17221/26/2019-VETMEDvet-201910-0005Development of an oral vaccine using recombinant viral haemorrhagic septicaemia virus glycoproteins produced in tobaccoTae Geum Kim0Bong Jo Kang1Sang Ik Park2Tae Jung Kim3Center for Jeongup Industry-Academy-Institute Cooperation, Chonbuk National University, Jeollabuk-do, Republic of KoreaOcean and Fisheries Research Institute, Jeju special self-governing province, Republic of KoreaCollege of Veterinary Medicine, Chonnam National University, Gwangju, Republic of KoreaCollege of Veterinary Medicine, Chonnam National University, Gwangju, Republic of KoreaThe viral haemorrhagic septicaemia virus (VHSV) causes high mortality in many marine and freshwater fish species, resulting in heavy economic losses in fish farming. Previously, cholera toxin B subunit (CTB)-fused recombinant viral haemorrhagic septicaemia virus glycoproteins (rec-VHSV-GPs) have been successfully expressed in tobacco, Nicotiana benthamiana. Here, we evaluated the potential of rec-VHSV-GPs as an oral vaccine against a live viral challenge. After immunisation of mice and fish (olive flounder, Paralichthys olivaceous) with those antigenic proteins in a feed additive form, the antibody titres were increased statistically, especially in the primed groups (P < 0.0001) in both the mouse and fish. After the viral challenge under low water temperature culture conditions (below 18 °C), the immunised fish were protected successfully against the challenge, showing a significantly lower mortality rate (P < 0.05). This result suggests that this plant-based immunisation system could induce an effective immune response. It could be used as a candidate to develop an oral vaccine for fish.https://vetmed.agriculturejournals.cz/artkey/vet-201910-0005_development-of-an-oral-vaccine-using-recombinant-viral-haemorrhagic-septicaemia-virus-glycoproteins-produced-in.phpflounderoral vaccineplant expressionviral haemorrhagic septicaemia virus |
| spellingShingle | Tae Geum Kim Bong Jo Kang Sang Ik Park Tae Jung Kim Development of an oral vaccine using recombinant viral haemorrhagic septicaemia virus glycoproteins produced in tobacco Veterinární Medicína flounder oral vaccine plant expression viral haemorrhagic septicaemia virus |
| title | Development of an oral vaccine using recombinant viral haemorrhagic septicaemia virus glycoproteins produced in tobacco |
| title_full | Development of an oral vaccine using recombinant viral haemorrhagic septicaemia virus glycoproteins produced in tobacco |
| title_fullStr | Development of an oral vaccine using recombinant viral haemorrhagic septicaemia virus glycoproteins produced in tobacco |
| title_full_unstemmed | Development of an oral vaccine using recombinant viral haemorrhagic septicaemia virus glycoproteins produced in tobacco |
| title_short | Development of an oral vaccine using recombinant viral haemorrhagic septicaemia virus glycoproteins produced in tobacco |
| title_sort | development of an oral vaccine using recombinant viral haemorrhagic septicaemia virus glycoproteins produced in tobacco |
| topic | flounder oral vaccine plant expression viral haemorrhagic septicaemia virus |
| url | https://vetmed.agriculturejournals.cz/artkey/vet-201910-0005_development-of-an-oral-vaccine-using-recombinant-viral-haemorrhagic-septicaemia-virus-glycoproteins-produced-in.php |
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