Antimicrobial and Isolation of Stigmasterol from Ethyl Acetate Extract of Musa Acuminate Calla Flowers

Banana blossom is traditionally used in northern Nigeria to treat a variety of ailments, including microbiological infections; reduces menstrual bleeding; aids in the treatment of diabetes, anaemia, and ulcers. Reduces anxiety, aids in weight loss, and produces an undetermined amount of filtrate dec...

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Main Authors: Akacha Usman Linus, Mohammed Hassan Shagal, Iliya Ibrahim Nkafamiya
Format: Article
Language:English
Published: Altezoro s.r.o. (Slovak Republic) and Publishing Center "Dialog" (Ukraine) 2024-02-01
Series:Traektoriâ Nauki
Subjects:
Online Access:https://pathofscience.org/index.php/ps/article/view/3025
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author Akacha Usman Linus
Mohammed Hassan Shagal
Iliya Ibrahim Nkafamiya
author_facet Akacha Usman Linus
Mohammed Hassan Shagal
Iliya Ibrahim Nkafamiya
author_sort Akacha Usman Linus
collection DOAJ
description Banana blossom is traditionally used in northern Nigeria to treat a variety of ailments, including microbiological infections; reduces menstrual bleeding; aids in the treatment of diabetes, anaemia, and ulcers. Reduces anxiety, aids in weight loss, and produces an undetermined amount of filtrate decoction to facilitate conception. As a result, the study intends to isolate secondary metabolites to identify the bioactive compound(s) present in banana flowers. Based on the antimicrobial activity results, the highest inhibitory concentration for ethyl acetate extracts of the Banana blossom flower is 12.5 mg/ml, as indicated below. Compounds are isolated using column chromatography. Isolation was performed using an ethyl acetate extract of the banana blossom flower. The fractions from the column are subjected to TLC testing to determine the separation of the component combination using a thin stationary phase supported by an inert backing, resulting in BF16 fractions. The goal of TLC is to produce well-defined, well-separated spots. Each component has a retention factor (RF), which joins fractions with equal RF values. We extracted stigmasterol from Musa acuminata calla ethyl acetate extracts and analysed it using NMR spectroscopy and FT-IR.
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spelling doaj.art-f8684664fe314d17953dcfbfba94ab9d2024-04-23T20:18:35ZengAltezoro s.r.o. (Slovak Republic) and Publishing Center "Dialog" (Ukraine)Traektoriâ Nauki2413-90092024-02-011025001500710.22178/pos.101-71147Antimicrobial and Isolation of Stigmasterol from Ethyl Acetate Extract of Musa Acuminate Calla FlowersAkacha Usman Linus0Mohammed Hassan Shagal1Iliya Ibrahim Nkafamiya2Adamawa State College of EducationModibbo Adama University YolaModibbo Adama University YolaBanana blossom is traditionally used in northern Nigeria to treat a variety of ailments, including microbiological infections; reduces menstrual bleeding; aids in the treatment of diabetes, anaemia, and ulcers. Reduces anxiety, aids in weight loss, and produces an undetermined amount of filtrate decoction to facilitate conception. As a result, the study intends to isolate secondary metabolites to identify the bioactive compound(s) present in banana flowers. Based on the antimicrobial activity results, the highest inhibitory concentration for ethyl acetate extracts of the Banana blossom flower is 12.5 mg/ml, as indicated below. Compounds are isolated using column chromatography. Isolation was performed using an ethyl acetate extract of the banana blossom flower. The fractions from the column are subjected to TLC testing to determine the separation of the component combination using a thin stationary phase supported by an inert backing, resulting in BF16 fractions. The goal of TLC is to produce well-defined, well-separated spots. Each component has a retention factor (RF), which joins fractions with equal RF values. We extracted stigmasterol from Musa acuminata calla ethyl acetate extracts and analysed it using NMR spectroscopy and FT-IR.https://pathofscience.org/index.php/ps/article/view/3025musa acuminata callaantimicrobial activitiesstigmasterolextraction
spellingShingle Akacha Usman Linus
Mohammed Hassan Shagal
Iliya Ibrahim Nkafamiya
Antimicrobial and Isolation of Stigmasterol from Ethyl Acetate Extract of Musa Acuminate Calla Flowers
Traektoriâ Nauki
musa acuminata calla
antimicrobial activities
stigmasterol
extraction
title Antimicrobial and Isolation of Stigmasterol from Ethyl Acetate Extract of Musa Acuminate Calla Flowers
title_full Antimicrobial and Isolation of Stigmasterol from Ethyl Acetate Extract of Musa Acuminate Calla Flowers
title_fullStr Antimicrobial and Isolation of Stigmasterol from Ethyl Acetate Extract of Musa Acuminate Calla Flowers
title_full_unstemmed Antimicrobial and Isolation of Stigmasterol from Ethyl Acetate Extract of Musa Acuminate Calla Flowers
title_short Antimicrobial and Isolation of Stigmasterol from Ethyl Acetate Extract of Musa Acuminate Calla Flowers
title_sort antimicrobial and isolation of stigmasterol from ethyl acetate extract of musa acuminate calla flowers
topic musa acuminata calla
antimicrobial activities
stigmasterol
extraction
url https://pathofscience.org/index.php/ps/article/view/3025
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AT mohammedhassanshagal antimicrobialandisolationofstigmasterolfromethylacetateextractofmusaacuminatecallaflowers
AT iliyaibrahimnkafamiya antimicrobialandisolationofstigmasterolfromethylacetateextractofmusaacuminatecallaflowers