Detection of Bartonella spp. in dogs after infection with Rickettsia rickettsii
Abstract Background Dynamics of infection by Bartonella and Rickettsia species, which are epidemiologically associated in dogs, have not been explored in a controlled setting. Objectives Describe an outbreak investigation of occult Bartonella spp. infection among a group of dogs, discovered after ex...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Journal of Veterinary Internal Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1111/jvim.15675 |
| _version_ | 1819147054332510208 |
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| author | Erin Lashnits Pradeep Neupane Ricardo G. Maggi Keith E. Linder Julie M. Bradley Nandhakumar Balakrishnan Brittany L. Southern Gabriel P. McKeon Ramaswamy Chandrashekar Edward B. Breitschwerdt |
| author_facet | Erin Lashnits Pradeep Neupane Ricardo G. Maggi Keith E. Linder Julie M. Bradley Nandhakumar Balakrishnan Brittany L. Southern Gabriel P. McKeon Ramaswamy Chandrashekar Edward B. Breitschwerdt |
| author_sort | Erin Lashnits |
| collection | DOAJ |
| description | Abstract Background Dynamics of infection by Bartonella and Rickettsia species, which are epidemiologically associated in dogs, have not been explored in a controlled setting. Objectives Describe an outbreak investigation of occult Bartonella spp. infection among a group of dogs, discovered after experimentally induced Rickettsia rickettsii (Rr) infection. Animals Six apparently healthy purpose‐bred Beagles obtained from a commercial vendor. Methods Retrospective and prospective study. Dogs were serially tested for Bartonella spp. and Rr using serology, culture, and PCR, over 3 study phases: 3 months before inoculation with Rr (retrospective), 6 weeks after inoculation with Rr (retrospective), and 8 months of follow‐up (prospective). Results Before Rr infection, 1 dog was Bartonella henselae (Bh) immunofluorescent antibody assay (IFA) seroreactive and 1 was Rickettsia spp. IFA seroreactive. After inoculation with Rr, all dogs developed mild Rocky Mountain spotted fever compatible with low‐dose Rr infection, seroconverted to Rickettsia spp. within 4‐11 days, and recovered within 1 week. When 1 dog developed ear tip vasculitis with intra‐lesional Bh, an investigation of Bartonella spp. infection was undertaken. All dogs had seroconverted to 1‐3 Bartonella spp. between 7 and 18 days after Rr inoculation. Between 4 and 8 months after Rr inoculation, Bh DNA was amplified from multiple tissues from 2 dogs, and Bartonella vinsonii subsp. berkhoffii (Bvb) DNA was amplified from 4 of 5 dogs' oral swabs. Conclusions and Clinical Importance Vector‐borne disease exposure was demonstrated in research dogs from a commercial vendor. Despite limitations, our results support the possibilities of recrudescence of chronic subclinical Bartonella spp. infection after Rr infection and horizontal direct‐contact transmission between dogs. |
| first_indexed | 2024-12-22T13:23:43Z |
| format | Article |
| id | doaj.art-f86dbfdd47a74c45b2b96f24c2d42c93 |
| institution | Directory Open Access Journal |
| issn | 0891-6640 1939-1676 |
| language | English |
| last_indexed | 2024-12-22T13:23:43Z |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Veterinary Internal Medicine |
| spelling | doaj.art-f86dbfdd47a74c45b2b96f24c2d42c932022-12-21T18:24:23ZengWileyJournal of Veterinary Internal Medicine0891-66401939-16762020-01-0134114515910.1111/jvim.15675Detection of Bartonella spp. in dogs after infection with Rickettsia rickettsiiErin Lashnits0Pradeep Neupane1Ricardo G. Maggi2Keith E. Linder3Julie M. Bradley4Nandhakumar Balakrishnan5Brittany L. Southern6Gabriel P. McKeon7Ramaswamy Chandrashekar8Edward B. Breitschwerdt9Comparative Biomedical Sciences, College of Veterinary Medicine North Carolina State University Raleigh North CarolinaDepartment of Clinical Sciences, College of Veterinary Medicine North Carolina State University Raleigh North CarolinaDepartment of Clinical Sciences, College of Veterinary Medicine North Carolina State University Raleigh North CarolinaDepartment of Population Health and Pathobiology, College of Veterinary Medicine North Carolina State University Raleigh North CarolinaDepartment of Clinical Sciences, College of Veterinary Medicine North Carolina State University Raleigh North CarolinaIntracellular Pathogens Research Laboratory, Comparative Medicine Institute, College of Veterinary Medicine North Carolina State University Raleigh North CarolinaLaboratory Animal Resources, College of Veterinary Medicine North Carolina State University Raleigh North CarolinaDepartment of Clinical Sciences, College of Veterinary Medicine North Carolina State University Raleigh North CarolinaIDEXX Laboratories, Inc Westbrook MaineDepartment of Clinical Sciences, College of Veterinary Medicine North Carolina State University Raleigh North CarolinaAbstract Background Dynamics of infection by Bartonella and Rickettsia species, which are epidemiologically associated in dogs, have not been explored in a controlled setting. Objectives Describe an outbreak investigation of occult Bartonella spp. infection among a group of dogs, discovered after experimentally induced Rickettsia rickettsii (Rr) infection. Animals Six apparently healthy purpose‐bred Beagles obtained from a commercial vendor. Methods Retrospective and prospective study. Dogs were serially tested for Bartonella spp. and Rr using serology, culture, and PCR, over 3 study phases: 3 months before inoculation with Rr (retrospective), 6 weeks after inoculation with Rr (retrospective), and 8 months of follow‐up (prospective). Results Before Rr infection, 1 dog was Bartonella henselae (Bh) immunofluorescent antibody assay (IFA) seroreactive and 1 was Rickettsia spp. IFA seroreactive. After inoculation with Rr, all dogs developed mild Rocky Mountain spotted fever compatible with low‐dose Rr infection, seroconverted to Rickettsia spp. within 4‐11 days, and recovered within 1 week. When 1 dog developed ear tip vasculitis with intra‐lesional Bh, an investigation of Bartonella spp. infection was undertaken. All dogs had seroconverted to 1‐3 Bartonella spp. between 7 and 18 days after Rr inoculation. Between 4 and 8 months after Rr inoculation, Bh DNA was amplified from multiple tissues from 2 dogs, and Bartonella vinsonii subsp. berkhoffii (Bvb) DNA was amplified from 4 of 5 dogs' oral swabs. Conclusions and Clinical Importance Vector‐borne disease exposure was demonstrated in research dogs from a commercial vendor. Despite limitations, our results support the possibilities of recrudescence of chronic subclinical Bartonella spp. infection after Rr infection and horizontal direct‐contact transmission between dogs.https://doi.org/10.1111/jvim.15675PCRrecrudescenceserologytransmission |
| spellingShingle | Erin Lashnits Pradeep Neupane Ricardo G. Maggi Keith E. Linder Julie M. Bradley Nandhakumar Balakrishnan Brittany L. Southern Gabriel P. McKeon Ramaswamy Chandrashekar Edward B. Breitschwerdt Detection of Bartonella spp. in dogs after infection with Rickettsia rickettsii Journal of Veterinary Internal Medicine PCR recrudescence serology transmission |
| title | Detection of Bartonella spp. in dogs after infection with Rickettsia rickettsii |
| title_full | Detection of Bartonella spp. in dogs after infection with Rickettsia rickettsii |
| title_fullStr | Detection of Bartonella spp. in dogs after infection with Rickettsia rickettsii |
| title_full_unstemmed | Detection of Bartonella spp. in dogs after infection with Rickettsia rickettsii |
| title_short | Detection of Bartonella spp. in dogs after infection with Rickettsia rickettsii |
| title_sort | detection of bartonella spp in dogs after infection with rickettsia rickettsii |
| topic | PCR recrudescence serology transmission |
| url | https://doi.org/10.1111/jvim.15675 |
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