Role for Gag-CA Interdomain Linker in Primate Lentiviral Replication
Gag proteins underlie retroviral replication by fulfilling numerous functional roles at various stages during viral life cycle. Out of the four mature proteins, Gag-capsid (CA) is a major component of viral particles, and has been most well studied biogenetically, biochemically and structurally. Gag...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-08-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2019.01831/full |
| _version_ | 1818290330076708864 |
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| author | Naoya Doi Takaaki Koma Akio Adachi Masako Nomaguchi |
| author_facet | Naoya Doi Takaaki Koma Akio Adachi Masako Nomaguchi |
| author_sort | Naoya Doi |
| collection | DOAJ |
| description | Gag proteins underlie retroviral replication by fulfilling numerous functional roles at various stages during viral life cycle. Out of the four mature proteins, Gag-capsid (CA) is a major component of viral particles, and has been most well studied biogenetically, biochemically and structurally. Gag-CA is composed of two structured domains, and also of a short stretch of disordered and flexible interdomain linker. While the two domains, namely, N-terminal and C-terminal domains (NTD and CTD), have been the central target for Gag research, the linker region connecting the two has been poorly studied. We recently have performed systemic mutational analyses on the Gag-CA linker region of HIV-1 by various experimental and in silico systems. In total, we have demonstrated that the linker region acts as a cis-modulator to optimize the Gag-related viral replication process. We also have noted, during the course of conducting the research project, that HIV-1 and SIVmac, belonging to distinct primate lentiviral lineages, share a similarly biologically active linker region with each other. In this brief article, we summarize and report the results obtained by mutational studies that are relevant to the functional significance of the interdomain linker of HIV/SIV Gag-CA. Based on this investigation, we discuss about the future directions of the research in this line. |
| first_indexed | 2024-12-13T02:26:27Z |
| format | Article |
| id | doaj.art-f86df23e18ed4f32be960050d556af53 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-13T02:26:27Z |
| publishDate | 2019-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-f86df23e18ed4f32be960050d556af532022-12-22T00:02:37ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2019-08-011010.3389/fmicb.2019.01831479908Role for Gag-CA Interdomain Linker in Primate Lentiviral ReplicationNaoya Doi0Takaaki Koma1Akio Adachi2Masako Nomaguchi3Department of Microbiology, Graduate School of Medical Science, Tokushima University, Tokushima, JapanDepartment of Microbiology, Graduate School of Medical Science, Tokushima University, Tokushima, JapanDepartment of Microbiology, Kansai Medical University, Osaka, JapanDepartment of Microbiology, Graduate School of Medical Science, Tokushima University, Tokushima, JapanGag proteins underlie retroviral replication by fulfilling numerous functional roles at various stages during viral life cycle. Out of the four mature proteins, Gag-capsid (CA) is a major component of viral particles, and has been most well studied biogenetically, biochemically and structurally. Gag-CA is composed of two structured domains, and also of a short stretch of disordered and flexible interdomain linker. While the two domains, namely, N-terminal and C-terminal domains (NTD and CTD), have been the central target for Gag research, the linker region connecting the two has been poorly studied. We recently have performed systemic mutational analyses on the Gag-CA linker region of HIV-1 by various experimental and in silico systems. In total, we have demonstrated that the linker region acts as a cis-modulator to optimize the Gag-related viral replication process. We also have noted, during the course of conducting the research project, that HIV-1 and SIVmac, belonging to distinct primate lentiviral lineages, share a similarly biologically active linker region with each other. In this brief article, we summarize and report the results obtained by mutational studies that are relevant to the functional significance of the interdomain linker of HIV/SIV Gag-CA. Based on this investigation, we discuss about the future directions of the research in this line.https://www.frontiersin.org/article/10.3389/fmicb.2019.01831/fullHIV/SIVGag-CAinterdomain linkerGag assemblyvirus productionearly infectivity |
| spellingShingle | Naoya Doi Takaaki Koma Akio Adachi Masako Nomaguchi Role for Gag-CA Interdomain Linker in Primate Lentiviral Replication Frontiers in Microbiology HIV/SIV Gag-CA interdomain linker Gag assembly virus production early infectivity |
| title | Role for Gag-CA Interdomain Linker in Primate Lentiviral Replication |
| title_full | Role for Gag-CA Interdomain Linker in Primate Lentiviral Replication |
| title_fullStr | Role for Gag-CA Interdomain Linker in Primate Lentiviral Replication |
| title_full_unstemmed | Role for Gag-CA Interdomain Linker in Primate Lentiviral Replication |
| title_short | Role for Gag-CA Interdomain Linker in Primate Lentiviral Replication |
| title_sort | role for gag ca interdomain linker in primate lentiviral replication |
| topic | HIV/SIV Gag-CA interdomain linker Gag assembly virus production early infectivity |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2019.01831/full |
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