Effect of Bacteriocin (ALC102) of Enterococcus faecium GRD AA on Biofilm Forming Listeria monocytogenes MTCC 657
Antilisterial bacteriocin producing strain were isolated from milk samples and were subjected to 16S rRNA sequencing and found to be of genus Enterococcus faecium. The bacteriocin ALC102 were partially purified by Amberlite XAD-16 adsorption followed by column chromatography. The biofilm formation c...
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Journal of Pure and Applied Microbiology
2022-03-01
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Series: | Journal of Pure and Applied Microbiology |
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Online Access: | https://microbiologyjournal.org/effect-of-bacteriocin-alc102-of-enterococcus-faecium-grd-aa-on-biofilm-forming-listeria-monocytogenes-mtcc-657/ |
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author | Arya Radhakrishnan Krishna Swathy Krishna Jayalekshmi Trisha Mary Pandipilly Antony Suganthi Ramasamy |
author_facet | Arya Radhakrishnan Krishna Swathy Krishna Jayalekshmi Trisha Mary Pandipilly Antony Suganthi Ramasamy |
author_sort | Arya Radhakrishnan Krishna |
collection | DOAJ |
description | Antilisterial bacteriocin producing strain were isolated from milk samples and were subjected to 16S rRNA sequencing and found to be of genus Enterococcus faecium. The bacteriocin ALC102 were partially purified by Amberlite XAD-16 adsorption followed by column chromatography. The biofilm formation capacity of Listeria monocytogenes MTCC 657 were evaluated by tube method and CV binding assay. Biofilm formation on different abiotic substrates were also evaluated. Among three substrates stainless steel had a strong biofilm formation followed by glass and aluminum foil. From the results of biofilm eradication studies, the bacteriocin ALC102 showed almost similar activity of commercial bacteriocin nisin on all the substrates at 45°C, 30°C, 4°C and -20°C. Based on CBD® biofilm eradication assay, the eradication potential of ALC102 and nisin were found to be similar on high (45°C) and freezing (-20°C) temperatures. From the study, antilisterial bacteriocin ALC102 found to be able to inhibit the biofilm formed Listeria monocytogenes MTCC 657 at different temperatures and different incubation periods (24h, 48h and 72h). The biofilm eradication potential of antilisterial bacteriocin ALC102 was similar to nisin. Neither incubation temperature nor incubation period doesn’t altered the activity of the bacteriocin. So this bacteriocin can be considered as a potential competitor in food industry and we strongly recommend the use of this bacteriocin from Enterococcus faecium GRD AA in the food preservation industry to a higher temperature (45°C) to freezing temperature (-20°C). |
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spelling | doaj.art-f86e4bb445244b868a285306213c8db62022-12-22T02:49:42ZengJournal of Pure and Applied MicrobiologyJournal of Pure and Applied Microbiology0973-75102581-690X2022-03-01161481493https://doi.org/10.22207/JPAM.16.1.46Effect of Bacteriocin (ALC102) of Enterococcus faecium GRD AA on Biofilm Forming Listeria monocytogenes MTCC 657Arya Radhakrishnan Krishnahttps://orcid.org/0000-0002-8101-7132Swathy Krishna Jayalekshmihttps://orcid.org/0000-0001-5566-390XTrisha Mary Pandipilly Antonyhttps://orcid.org/0000-0001-9539-3473Suganthi Ramasamyhttps://orcid.org/0000-0001-8654-4145Antilisterial bacteriocin producing strain were isolated from milk samples and were subjected to 16S rRNA sequencing and found to be of genus Enterococcus faecium. The bacteriocin ALC102 were partially purified by Amberlite XAD-16 adsorption followed by column chromatography. The biofilm formation capacity of Listeria monocytogenes MTCC 657 were evaluated by tube method and CV binding assay. Biofilm formation on different abiotic substrates were also evaluated. Among three substrates stainless steel had a strong biofilm formation followed by glass and aluminum foil. From the results of biofilm eradication studies, the bacteriocin ALC102 showed almost similar activity of commercial bacteriocin nisin on all the substrates at 45°C, 30°C, 4°C and -20°C. Based on CBD® biofilm eradication assay, the eradication potential of ALC102 and nisin were found to be similar on high (45°C) and freezing (-20°C) temperatures. From the study, antilisterial bacteriocin ALC102 found to be able to inhibit the biofilm formed Listeria monocytogenes MTCC 657 at different temperatures and different incubation periods (24h, 48h and 72h). The biofilm eradication potential of antilisterial bacteriocin ALC102 was similar to nisin. Neither incubation temperature nor incubation period doesn’t altered the activity of the bacteriocin. So this bacteriocin can be considered as a potential competitor in food industry and we strongly recommend the use of this bacteriocin from Enterococcus faecium GRD AA in the food preservation industry to a higher temperature (45°C) to freezing temperature (-20°C).https://microbiologyjournal.org/effect-of-bacteriocin-alc102-of-enterococcus-faecium-grd-aa-on-biofilm-forming-listeria-monocytogenes-mtcc-657/enterococcus faeciumbacteriocinlisteria monocytogenesbiofilmnisin |
spellingShingle | Arya Radhakrishnan Krishna Swathy Krishna Jayalekshmi Trisha Mary Pandipilly Antony Suganthi Ramasamy Effect of Bacteriocin (ALC102) of Enterococcus faecium GRD AA on Biofilm Forming Listeria monocytogenes MTCC 657 Journal of Pure and Applied Microbiology enterococcus faecium bacteriocin listeria monocytogenes biofilm nisin |
title | Effect of Bacteriocin (ALC102) of Enterococcus faecium GRD AA on Biofilm Forming Listeria monocytogenes MTCC 657 |
title_full | Effect of Bacteriocin (ALC102) of Enterococcus faecium GRD AA on Biofilm Forming Listeria monocytogenes MTCC 657 |
title_fullStr | Effect of Bacteriocin (ALC102) of Enterococcus faecium GRD AA on Biofilm Forming Listeria monocytogenes MTCC 657 |
title_full_unstemmed | Effect of Bacteriocin (ALC102) of Enterococcus faecium GRD AA on Biofilm Forming Listeria monocytogenes MTCC 657 |
title_short | Effect of Bacteriocin (ALC102) of Enterococcus faecium GRD AA on Biofilm Forming Listeria monocytogenes MTCC 657 |
title_sort | effect of bacteriocin alc102 of enterococcus faecium grd aa on biofilm forming listeria monocytogenes mtcc 657 |
topic | enterococcus faecium bacteriocin listeria monocytogenes biofilm nisin |
url | https://microbiologyjournal.org/effect-of-bacteriocin-alc102-of-enterococcus-faecium-grd-aa-on-biofilm-forming-listeria-monocytogenes-mtcc-657/ |
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