Absolute quantification of serum microRNA-122 and its correlation with liver inflammation grade and serum alanine aminotransferase in chronic hepatitis C patients
Objectives: MicroRNA-122 has been shown to be crucial for efficient HCV RNA replication in vitro. Pretreatment intrahepatic microRNA-122 levels in chronic hepatitis C (CHC) patients have been associated with the outcomes of interferon therapy. Here, we determined microRNA-122 serum levels in CHC pat...
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| Format: | Article |
| Language: | English |
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Elsevier
2015-01-01
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| Series: | International Journal of Infectious Diseases |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1201971214016865 |
| _version_ | 1828531298103197696 |
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| author | Jiang-hua Wang Dong Jiang Hui-yng Rao Jing-min Zhao Yu Wang Lai Wei |
| author_facet | Jiang-hua Wang Dong Jiang Hui-yng Rao Jing-min Zhao Yu Wang Lai Wei |
| author_sort | Jiang-hua Wang |
| collection | DOAJ |
| description | Objectives: MicroRNA-122 has been shown to be crucial for efficient HCV RNA replication in vitro. Pretreatment intrahepatic microRNA-122 levels in chronic hepatitis C (CHC) patients have been associated with the outcomes of interferon therapy. Here, we determined microRNA-122 serum levels in CHC patients and healthy donors using an absolute quantification approach and evaluated the correlation with liver inflammation grades and serum alanine aminotransferase (ALT) levels.
Methods: Serum samples were collected from 105 treatment-naive CHC patients, 11 acute hepatitis patients, and 33 healthy donors. Serum microRNA-122 was measured using the TaqMan RT-qPCR. The cycle threshold values were converted to copy numbers by drawing a standard curve using a chemical synthetic standard. For accurate quantification, copy numbers were further normalized according to the recovery ratios of spiked-in cel-miR-39.
Results: Serum levels of microRNA-122 were significantly higher in acute hepatitis and CHC patients than in healthy donors (p < 0.001). However, there was no significant association between microRNA-122 and ALT serum levels or liver inflammation grades.
Conclusions: The present study showed that serum microRNA-122 was elevated in acute and chronic hepatitis patients. However, this biomarker for acute liver injury did not reflect the liver inflammation activity in CHC patients. |
| first_indexed | 2024-12-11T22:35:39Z |
| format | Article |
| id | doaj.art-f86eedde9273402b923e8189e1afb9d5 |
| institution | Directory Open Access Journal |
| issn | 1201-9712 1878-3511 |
| language | English |
| last_indexed | 2024-12-11T22:35:39Z |
| publishDate | 2015-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | International Journal of Infectious Diseases |
| spelling | doaj.art-f86eedde9273402b923e8189e1afb9d52022-12-22T00:47:58ZengElsevierInternational Journal of Infectious Diseases1201-97121878-35112015-01-0130C525610.1016/j.ijid.2014.09.020Absolute quantification of serum microRNA-122 and its correlation with liver inflammation grade and serum alanine aminotransferase in chronic hepatitis C patientsJiang-hua Wang0Dong Jiang1Hui-yng Rao2Jing-min Zhao3Yu Wang4Lai Wei5Peking University People's Hospital, Peking University Hepatology Institute, Beijing Key Laboratory of Hepatitis C and Immunotherapy for Liver Diseases, No. 11, Xizhimen South Street, Beijing 100044, ChinaPeking University People's Hospital, Peking University Hepatology Institute, Beijing Key Laboratory of Hepatitis C and Immunotherapy for Liver Diseases, No. 11, Xizhimen South Street, Beijing 100044, ChinaPeking University People's Hospital, Peking University Hepatology Institute, Beijing Key Laboratory of Hepatitis C and Immunotherapy for Liver Diseases, No. 11, Xizhimen South Street, Beijing 100044, China302 Military Hospital of China, Beijing, ChinaChinese Center for Disease Control and Prevention, 155 Changbai Road, Changping District, Beijing 102206, ChinaPeking University People's Hospital, Peking University Hepatology Institute, Beijing Key Laboratory of Hepatitis C and Immunotherapy for Liver Diseases, No. 11, Xizhimen South Street, Beijing 100044, ChinaObjectives: MicroRNA-122 has been shown to be crucial for efficient HCV RNA replication in vitro. Pretreatment intrahepatic microRNA-122 levels in chronic hepatitis C (CHC) patients have been associated with the outcomes of interferon therapy. Here, we determined microRNA-122 serum levels in CHC patients and healthy donors using an absolute quantification approach and evaluated the correlation with liver inflammation grades and serum alanine aminotransferase (ALT) levels. Methods: Serum samples were collected from 105 treatment-naive CHC patients, 11 acute hepatitis patients, and 33 healthy donors. Serum microRNA-122 was measured using the TaqMan RT-qPCR. The cycle threshold values were converted to copy numbers by drawing a standard curve using a chemical synthetic standard. For accurate quantification, copy numbers were further normalized according to the recovery ratios of spiked-in cel-miR-39. Results: Serum levels of microRNA-122 were significantly higher in acute hepatitis and CHC patients than in healthy donors (p < 0.001). However, there was no significant association between microRNA-122 and ALT serum levels or liver inflammation grades. Conclusions: The present study showed that serum microRNA-122 was elevated in acute and chronic hepatitis patients. However, this biomarker for acute liver injury did not reflect the liver inflammation activity in CHC patients.http://www.sciencedirect.com/science/article/pii/S1201971214016865MicroRNA-122Chronic hepatitis CALTLiver inflammation |
| spellingShingle | Jiang-hua Wang Dong Jiang Hui-yng Rao Jing-min Zhao Yu Wang Lai Wei Absolute quantification of serum microRNA-122 and its correlation with liver inflammation grade and serum alanine aminotransferase in chronic hepatitis C patients International Journal of Infectious Diseases MicroRNA-122 Chronic hepatitis C ALT Liver inflammation |
| title | Absolute quantification of serum microRNA-122 and its correlation with liver inflammation grade and serum alanine aminotransferase in chronic hepatitis C patients |
| title_full | Absolute quantification of serum microRNA-122 and its correlation with liver inflammation grade and serum alanine aminotransferase in chronic hepatitis C patients |
| title_fullStr | Absolute quantification of serum microRNA-122 and its correlation with liver inflammation grade and serum alanine aminotransferase in chronic hepatitis C patients |
| title_full_unstemmed | Absolute quantification of serum microRNA-122 and its correlation with liver inflammation grade and serum alanine aminotransferase in chronic hepatitis C patients |
| title_short | Absolute quantification of serum microRNA-122 and its correlation with liver inflammation grade and serum alanine aminotransferase in chronic hepatitis C patients |
| title_sort | absolute quantification of serum microrna 122 and its correlation with liver inflammation grade and serum alanine aminotransferase in chronic hepatitis c patients |
| topic | MicroRNA-122 Chronic hepatitis C ALT Liver inflammation |
| url | http://www.sciencedirect.com/science/article/pii/S1201971214016865 |
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