Generation of two induced pluripotent stem cell lines from dilated cardiomyopathy patients carrying TTN mutations
Dilated cardiomyopathy (DCM) is a common heart disease that can lead to heart failure and sudden cardiac death. Mutations in the TTN gene are the most frequent cause of DCM. Here, we generated two human induced pluripotent stem cell (iPSC) lines from the peripheral blood mononuclear cells (PBMCs) of...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-12-01
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| Series: | Stem Cell Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506122002902 |
| _version_ | 1811206342619168768 |
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| author | Tina Tianbo Zhang Shane Rui Zhao Christina Alamana Mengcheng Shen Victoria Parikh Matthew T. Wheeler Joseph C. Wu |
| author_facet | Tina Tianbo Zhang Shane Rui Zhao Christina Alamana Mengcheng Shen Victoria Parikh Matthew T. Wheeler Joseph C. Wu |
| author_sort | Tina Tianbo Zhang |
| collection | DOAJ |
| description | Dilated cardiomyopathy (DCM) is a common heart disease that can lead to heart failure and sudden cardiac death. Mutations in the TTN gene are the most frequent cause of DCM. Here, we generated two human induced pluripotent stem cell (iPSC) lines from the peripheral blood mononuclear cells (PBMCs) of two DCM patients carrying c.94816C>T and c.104188A>G mutations in TTN, respectively. The two lines exhibited a normal morphology, full expression of pluripotency markers, a normal karyotype and the ability of trilineage differentiation. The two lines can serve as useful tools for drug screening and mechanism studies on DCM. |
| first_indexed | 2024-04-12T03:45:47Z |
| format | Article |
| id | doaj.art-f8723fa1a65b4bbbb48c21b9e454ddf6 |
| institution | Directory Open Access Journal |
| issn | 1873-5061 |
| language | English |
| last_indexed | 2024-04-12T03:45:47Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj.art-f8723fa1a65b4bbbb48c21b9e454ddf62022-12-22T03:49:08ZengElsevierStem Cell Research1873-50612022-12-0165102941Generation of two induced pluripotent stem cell lines from dilated cardiomyopathy patients carrying TTN mutationsTina Tianbo Zhang0Shane Rui Zhao1Christina Alamana2Mengcheng Shen3Victoria Parikh4Matthew T. Wheeler5Joseph C. Wu6Stanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA, USA; Division of Cardiovascular Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, CA, USAStanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA, USA; Division of Cardiovascular Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, CA, USAStanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA, USA; Division of Cardiovascular Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, CA, USAStanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA, USA; Division of Cardiovascular Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, CA, USAStanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA, USA; Division of Cardiovascular Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, CA, USAStanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA, USA; Division of Cardiovascular Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, CA, USAStanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA, USA; Division of Cardiovascular Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, CA, USA; Corresponding author at: 265 Campus Drive, G1120B, Stanford, CA 94305, USA.Dilated cardiomyopathy (DCM) is a common heart disease that can lead to heart failure and sudden cardiac death. Mutations in the TTN gene are the most frequent cause of DCM. Here, we generated two human induced pluripotent stem cell (iPSC) lines from the peripheral blood mononuclear cells (PBMCs) of two DCM patients carrying c.94816C>T and c.104188A>G mutations in TTN, respectively. The two lines exhibited a normal morphology, full expression of pluripotency markers, a normal karyotype and the ability of trilineage differentiation. The two lines can serve as useful tools for drug screening and mechanism studies on DCM.http://www.sciencedirect.com/science/article/pii/S1873506122002902 |
| spellingShingle | Tina Tianbo Zhang Shane Rui Zhao Christina Alamana Mengcheng Shen Victoria Parikh Matthew T. Wheeler Joseph C. Wu Generation of two induced pluripotent stem cell lines from dilated cardiomyopathy patients carrying TTN mutations Stem Cell Research |
| title | Generation of two induced pluripotent stem cell lines from dilated cardiomyopathy patients carrying TTN mutations |
| title_full | Generation of two induced pluripotent stem cell lines from dilated cardiomyopathy patients carrying TTN mutations |
| title_fullStr | Generation of two induced pluripotent stem cell lines from dilated cardiomyopathy patients carrying TTN mutations |
| title_full_unstemmed | Generation of two induced pluripotent stem cell lines from dilated cardiomyopathy patients carrying TTN mutations |
| title_short | Generation of two induced pluripotent stem cell lines from dilated cardiomyopathy patients carrying TTN mutations |
| title_sort | generation of two induced pluripotent stem cell lines from dilated cardiomyopathy patients carrying ttn mutations |
| url | http://www.sciencedirect.com/science/article/pii/S1873506122002902 |
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