Establishment of a Rapid Micropropagation System for <i>Kaempferia parviflora</i> Wall. Ex Baker: Phytochemical Analysis of Leaf Extracts and Evaluation of Biological Activities
This study aimed to establish a rapid in vitro plant regeneration method from rhizome buds of <i>Kaempferia parviflora</i> to obtain the valuable secondary metabolites with antioxidant and enzyme inhibition properties. The disinfection effect of silver oxide nanoparticles (AgO NPs) on rh...
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MDPI AG
2021-04-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/10/4/698 |
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| author | Han-Yong Park Kyung-Su Kim Gunes Ak Gokhan Zengin Zoltán Cziáky József Jekő Kathalingam Adaikalam Kihwan Song Doo-Hwan Kim Iyyakkannu Sivanesan |
| author_facet | Han-Yong Park Kyung-Su Kim Gunes Ak Gokhan Zengin Zoltán Cziáky József Jekő Kathalingam Adaikalam Kihwan Song Doo-Hwan Kim Iyyakkannu Sivanesan |
| author_sort | Han-Yong Park |
| collection | DOAJ |
| description | This study aimed to establish a rapid in vitro plant regeneration method from rhizome buds of <i>Kaempferia parviflora</i> to obtain the valuable secondary metabolites with antioxidant and enzyme inhibition properties. The disinfection effect of silver oxide nanoparticles (AgO NPs) on rhizome and effects of plant growth regulators on shoot multiplication and subsequent rooting were investigated. Surface sterilization of rhizome buds with sodium hypochlorite was insufficient to control contamination. However, immersing rhizome buds in 100 mg L<sup>−1</sup> AgO NPs for 60 min eliminated contamination without affecting the survival of explants. The number of shoots (12.2) produced per rhizome bud was higher in Murashige and Skoog (MS) medium containing 8 µM of 6-Benzyladenine (6-BA) and 0.5 µM of Thidiazuron (TDZ) than other treatments. The highest number of roots (24), with a mean root length of 7.8 cm and the maximum shoot length (9.8 cm), were obtained on medium MS with 2 µM of Indole-3-butyric acid (IBA). A survival rate of 98% was attained when plantlets of <i>K. parviflora</i> were acclimatized in a growth room. Liquid chromatography with tandem mass spectrometry (LC-MS/MS) was used to determine the chemical profile of <i>K. parviflora</i> leaf extracts. Results showed that several biologically active flavonoids reported in rhizomes were also present in leaf tissues of both in vitro cultured and ex vitro (greenhouse-grown) plantlets of <i>K. parviflora</i>. We found 40 and 36 compounds in in vitro cultured and ex vitro grown leaf samples, respectively. Greenhouse leaves exhibited more potent antioxidant activities than leaves from in vitro cultures. A higher acetylcholinesterase inhibitory ability was obtained for greenhouse leaves (1.07 mg/mL). However, leaves from in vitro cultures exhibited stronger butyrylcholinesterase inhibitory abilities. These results suggest that leaves of <i>K. parviflora</i>, as major byproducts of black ginger cultivation, could be used as valuable alternative sources for extracting bioactive compounds. |
| first_indexed | 2024-03-10T12:36:18Z |
| format | Article |
| id | doaj.art-f872f6b706b646f690ff83fc620e09d6 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T12:36:18Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
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| series | Plants |
| spelling | doaj.art-f872f6b706b646f690ff83fc620e09d62023-11-21T14:15:15ZengMDPI AGPlants2223-77472021-04-0110469810.3390/plants10040698Establishment of a Rapid Micropropagation System for <i>Kaempferia parviflora</i> Wall. Ex Baker: Phytochemical Analysis of Leaf Extracts and Evaluation of Biological ActivitiesHan-Yong Park0Kyung-Su Kim1Gunes Ak2Gokhan Zengin3Zoltán Cziáky4József Jekő5Kathalingam Adaikalam6Kihwan Song7Doo-Hwan Kim8Iyyakkannu Sivanesan9Department of Bioresource Engineering, Sejong University, 209 Neungdong-ro, Gwangjin-gu, Seoul 05006, KoreaDepartment of Bioresources and Food Science, Institute of Natural Science and Agriculture, Konkuk University, Seoul 05029, KoreaDepartment of Biology, Faculty of Science, Selcuk University, 42130 Konya, TurkeyDepartment of Biology, Faculty of Science, Selcuk University, 42130 Konya, TurkeyAgricultural and Molecular Research and Service Institute, University of Nyíregyháza, 4400 Nyíregyháza, HungaryAgricultural and Molecular Research and Service Institute, University of Nyíregyháza, 4400 Nyíregyháza, HungaryMillimeter-wave Innovation Technology (MINT) Research Center, Dongguk University-Seoul, Seoul 04620, KoreaDepartment of Bioresource Engineering, Sejong University, 209 Neungdong-ro, Gwangjin-gu, Seoul 05006, KoreaDepartment of Bioresources and Food Science, Institute of Natural Science and Agriculture, Konkuk University, Seoul 05029, KoreaDepartment of Bioresources and Food Science, Institute of Natural Science and Agriculture, Konkuk University, Seoul 05029, KoreaThis study aimed to establish a rapid in vitro plant regeneration method from rhizome buds of <i>Kaempferia parviflora</i> to obtain the valuable secondary metabolites with antioxidant and enzyme inhibition properties. The disinfection effect of silver oxide nanoparticles (AgO NPs) on rhizome and effects of plant growth regulators on shoot multiplication and subsequent rooting were investigated. Surface sterilization of rhizome buds with sodium hypochlorite was insufficient to control contamination. However, immersing rhizome buds in 100 mg L<sup>−1</sup> AgO NPs for 60 min eliminated contamination without affecting the survival of explants. The number of shoots (12.2) produced per rhizome bud was higher in Murashige and Skoog (MS) medium containing 8 µM of 6-Benzyladenine (6-BA) and 0.5 µM of Thidiazuron (TDZ) than other treatments. The highest number of roots (24), with a mean root length of 7.8 cm and the maximum shoot length (9.8 cm), were obtained on medium MS with 2 µM of Indole-3-butyric acid (IBA). A survival rate of 98% was attained when plantlets of <i>K. parviflora</i> were acclimatized in a growth room. Liquid chromatography with tandem mass spectrometry (LC-MS/MS) was used to determine the chemical profile of <i>K. parviflora</i> leaf extracts. Results showed that several biologically active flavonoids reported in rhizomes were also present in leaf tissues of both in vitro cultured and ex vitro (greenhouse-grown) plantlets of <i>K. parviflora</i>. We found 40 and 36 compounds in in vitro cultured and ex vitro grown leaf samples, respectively. Greenhouse leaves exhibited more potent antioxidant activities than leaves from in vitro cultures. A higher acetylcholinesterase inhibitory ability was obtained for greenhouse leaves (1.07 mg/mL). However, leaves from in vitro cultures exhibited stronger butyrylcholinesterase inhibitory abilities. These results suggest that leaves of <i>K. parviflora</i>, as major byproducts of black ginger cultivation, could be used as valuable alternative sources for extracting bioactive compounds.https://www.mdpi.com/2223-7747/10/4/698micropropagationsilver oxide nanoparticlesflavonoidsantioxidant activityenzyme inhibition<i>Kaempferia parviflora</i> |
| spellingShingle | Han-Yong Park Kyung-Su Kim Gunes Ak Gokhan Zengin Zoltán Cziáky József Jekő Kathalingam Adaikalam Kihwan Song Doo-Hwan Kim Iyyakkannu Sivanesan Establishment of a Rapid Micropropagation System for <i>Kaempferia parviflora</i> Wall. Ex Baker: Phytochemical Analysis of Leaf Extracts and Evaluation of Biological Activities Plants micropropagation silver oxide nanoparticles flavonoids antioxidant activity enzyme inhibition <i>Kaempferia parviflora</i> |
| title | Establishment of a Rapid Micropropagation System for <i>Kaempferia parviflora</i> Wall. Ex Baker: Phytochemical Analysis of Leaf Extracts and Evaluation of Biological Activities |
| title_full | Establishment of a Rapid Micropropagation System for <i>Kaempferia parviflora</i> Wall. Ex Baker: Phytochemical Analysis of Leaf Extracts and Evaluation of Biological Activities |
| title_fullStr | Establishment of a Rapid Micropropagation System for <i>Kaempferia parviflora</i> Wall. Ex Baker: Phytochemical Analysis of Leaf Extracts and Evaluation of Biological Activities |
| title_full_unstemmed | Establishment of a Rapid Micropropagation System for <i>Kaempferia parviflora</i> Wall. Ex Baker: Phytochemical Analysis of Leaf Extracts and Evaluation of Biological Activities |
| title_short | Establishment of a Rapid Micropropagation System for <i>Kaempferia parviflora</i> Wall. Ex Baker: Phytochemical Analysis of Leaf Extracts and Evaluation of Biological Activities |
| title_sort | establishment of a rapid micropropagation system for i kaempferia parviflora i wall ex baker phytochemical analysis of leaf extracts and evaluation of biological activities |
| topic | micropropagation silver oxide nanoparticles flavonoids antioxidant activity enzyme inhibition <i>Kaempferia parviflora</i> |
| url | https://www.mdpi.com/2223-7747/10/4/698 |
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