Chaihu-Shugan-San exerts an antidepressive effect by downregulating miR-124 and releasing inhibition of the MAPK14 and Gria3 signaling pathways
Dysregulation of miR-124 has been reported to be involved in the pathophysiology of depression. Chaihu-Shugan-San, a traditional Chinese medicine, has antidepressive activity; however, the underlying mechanisms remain unclear. In this study, to generate a rodent model of depression, rats were subjec...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2018-01-01
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| Series: | Neural Regeneration Research |
| Subjects: | |
| Online Access: | http://www.nrronline.org/article.asp?issn=1673-5374;year=2018;volume=13;issue=5;spage=837;epage=845;aulast=Liu |
| _version_ | 1828966539524571136 |
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| author | Qiong Liu Ning-Ning Sun Zheng-Zhi Wu Da-Hua Fan Mei-Qun Cao |
| author_facet | Qiong Liu Ning-Ning Sun Zheng-Zhi Wu Da-Hua Fan Mei-Qun Cao |
| author_sort | Qiong Liu |
| collection | DOAJ |
| description | Dysregulation of miR-124 has been reported to be involved in the pathophysiology of depression. Chaihu-Shugan-San, a traditional Chinese medicine, has antidepressive activity; however, the underlying mechanisms remain unclear. In this study, to generate a rodent model of depression, rats were subjected to a combination of solitary confinement and chronic unpredictable mild stress for 28 days. Rats were intragastrically administered Chaihu-Shugan-San (2.835 mL/kg/d) for 4 weeks, once a day. Real-time reverse-transcription quantitative polymerase chain reaction, miRNA microarray, western blot assay and transmission electron microscopy demonstrated that Chaihu-Shugan-San downregulated miR-124 expression and upregulated the mRNA and protein levels of mitogen-activated protein kinase 14 (MAPK14) and glutamate receptor subunit 3 (Gria3). Chaihu-Shugan-San also promoted synapse formation in the hippocampus. The open field test, sucrose consumption test and forced swimming test were used to assess depression-like behavior. After intragastric administration of Chaihu-Shugan-San, sucrose consumption increased, while the depressive behaviors were substantially reduced. Together, these findings suggest that Chaihu-Shugan-San exerts an antidepressant-like effect by downregulating miR-124 expression and by releasing the inhibition of the MAPK14 and Gria3 signaling pathways. |
| first_indexed | 2024-12-14T11:32:56Z |
| format | Article |
| id | doaj.art-f8758978510741179ac7977c1c5e56aa |
| institution | Directory Open Access Journal |
| issn | 1673-5374 |
| language | English |
| last_indexed | 2024-12-14T11:32:56Z |
| publishDate | 2018-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Neural Regeneration Research |
| spelling | doaj.art-f8758978510741179ac7977c1c5e56aa2022-12-21T23:03:12ZengWolters Kluwer Medknow PublicationsNeural Regeneration Research1673-53742018-01-0113583784510.4103/1673-5374.232478Chaihu-Shugan-San exerts an antidepressive effect by downregulating miR-124 and releasing inhibition of the MAPK14 and Gria3 signaling pathwaysQiong LiuNing-Ning SunZheng-Zhi WuDa-Hua FanMei-Qun CaoDysregulation of miR-124 has been reported to be involved in the pathophysiology of depression. Chaihu-Shugan-San, a traditional Chinese medicine, has antidepressive activity; however, the underlying mechanisms remain unclear. In this study, to generate a rodent model of depression, rats were subjected to a combination of solitary confinement and chronic unpredictable mild stress for 28 days. Rats were intragastrically administered Chaihu-Shugan-San (2.835 mL/kg/d) for 4 weeks, once a day. Real-time reverse-transcription quantitative polymerase chain reaction, miRNA microarray, western blot assay and transmission electron microscopy demonstrated that Chaihu-Shugan-San downregulated miR-124 expression and upregulated the mRNA and protein levels of mitogen-activated protein kinase 14 (MAPK14) and glutamate receptor subunit 3 (Gria3). Chaihu-Shugan-San also promoted synapse formation in the hippocampus. The open field test, sucrose consumption test and forced swimming test were used to assess depression-like behavior. After intragastric administration of Chaihu-Shugan-San, sucrose consumption increased, while the depressive behaviors were substantially reduced. Together, these findings suggest that Chaihu-Shugan-San exerts an antidepressant-like effect by downregulating miR-124 expression and by releasing the inhibition of the MAPK14 and Gria3 signaling pathways.http://www.nrronline.org/article.asp?issn=1673-5374;year=2018;volume=13;issue=5;spage=837;epage=845;aulast=Liunerve regeneration; traditional Chinese medicine; Chaihu-Shugan-San; depression; open-field test; sucrose consumption test; forced swimming test; miR-124; neural plasticity; MAPK14; Gria3; neural regeneration |
| spellingShingle | Qiong Liu Ning-Ning Sun Zheng-Zhi Wu Da-Hua Fan Mei-Qun Cao Chaihu-Shugan-San exerts an antidepressive effect by downregulating miR-124 and releasing inhibition of the MAPK14 and Gria3 signaling pathways Neural Regeneration Research nerve regeneration; traditional Chinese medicine; Chaihu-Shugan-San; depression; open-field test; sucrose consumption test; forced swimming test; miR-124; neural plasticity; MAPK14; Gria3; neural regeneration |
| title | Chaihu-Shugan-San exerts an antidepressive effect by downregulating miR-124 and releasing inhibition of the MAPK14 and Gria3 signaling pathways |
| title_full | Chaihu-Shugan-San exerts an antidepressive effect by downregulating miR-124 and releasing inhibition of the MAPK14 and Gria3 signaling pathways |
| title_fullStr | Chaihu-Shugan-San exerts an antidepressive effect by downregulating miR-124 and releasing inhibition of the MAPK14 and Gria3 signaling pathways |
| title_full_unstemmed | Chaihu-Shugan-San exerts an antidepressive effect by downregulating miR-124 and releasing inhibition of the MAPK14 and Gria3 signaling pathways |
| title_short | Chaihu-Shugan-San exerts an antidepressive effect by downregulating miR-124 and releasing inhibition of the MAPK14 and Gria3 signaling pathways |
| title_sort | chaihu shugan san exerts an antidepressive effect by downregulating mir 124 and releasing inhibition of the mapk14 and gria3 signaling pathways |
| topic | nerve regeneration; traditional Chinese medicine; Chaihu-Shugan-San; depression; open-field test; sucrose consumption test; forced swimming test; miR-124; neural plasticity; MAPK14; Gria3; neural regeneration |
| url | http://www.nrronline.org/article.asp?issn=1673-5374;year=2018;volume=13;issue=5;spage=837;epage=845;aulast=Liu |
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