Bee Venom Alleviates Atopic Dermatitis Symptoms through the Upregulation of Decay-Accelerating Factor (DAF/CD55)
Bee venom (BV)—a complex mixture of peptides and toxic proteins including phospholipase A2 and melittin—promotes blood clotting. In this study, we investigated the anti-atopic properties of BV and the mechanism associated with its regulation of the complement system. BV treatment...
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| Format: | Article |
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MDPI AG
2019-04-01
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| Series: | Toxins |
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| Online Access: | https://www.mdpi.com/2072-6651/11/5/239 |
| _version_ | 1798034692396548096 |
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| author | Yenny Kim Youn-Woo Lee Hangeun Kim Dae Kyun Chung |
| author_facet | Yenny Kim Youn-Woo Lee Hangeun Kim Dae Kyun Chung |
| author_sort | Yenny Kim |
| collection | DOAJ |
| description | Bee venom (BV)—a complex mixture of peptides and toxic proteins including phospholipase A2 and melittin—promotes blood clotting. In this study, we investigated the anti-atopic properties of BV and the mechanism associated with its regulation of the complement system. BV treatment upregulated the mRNA and protein levels of CD55 in THP-1 cells. Further experiments revealed that the phosphorylation of ERK was associated with upregulation of CD55. A complement-dependent cytotoxicity assay and a bacteria-killing assay showed that BV inactivated the complement system through the induction of CD55. The serum levels of C3 convertase (C3C) and Membrane attack complex (MAC) increased, while CD55 decreased in mice with AD-like lesions from DNCB treatment. However, the levels were inverted when the AD-like mice were treated with BV using subcutaneous injection, and we observed that the AD symptoms were alleviated. BV is often used to treat AD but its mechanism has not been elucidated. Here, we suggest that BV alleviates AD through the inactivation of the complement system, especially by the induction of CD55. |
| first_indexed | 2024-04-11T20:47:45Z |
| format | Article |
| id | doaj.art-f875c0dfb30348229e1e1be23204920f |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-04-11T20:47:45Z |
| publishDate | 2019-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-f875c0dfb30348229e1e1be23204920f2022-12-22T04:03:59ZengMDPI AGToxins2072-66512019-04-0111523910.3390/toxins11050239toxins11050239Bee Venom Alleviates Atopic Dermatitis Symptoms through the Upregulation of Decay-Accelerating Factor (DAF/CD55)Yenny Kim0Youn-Woo Lee1Hangeun Kim2Dae Kyun Chung3Graduate School of Biotechnology, Kyung Hee University, Yongin 17104, KoreaGraduate School of Biotechnology, Kyung Hee University, Yongin 17104, KoreaSkin Biotechnology Center, Kyung Hee University, Yongin 17104, KoreaGraduate School of Biotechnology, Kyung Hee University, Yongin 17104, KoreaBee venom (BV)—a complex mixture of peptides and toxic proteins including phospholipase A2 and melittin—promotes blood clotting. In this study, we investigated the anti-atopic properties of BV and the mechanism associated with its regulation of the complement system. BV treatment upregulated the mRNA and protein levels of CD55 in THP-1 cells. Further experiments revealed that the phosphorylation of ERK was associated with upregulation of CD55. A complement-dependent cytotoxicity assay and a bacteria-killing assay showed that BV inactivated the complement system through the induction of CD55. The serum levels of C3 convertase (C3C) and Membrane attack complex (MAC) increased, while CD55 decreased in mice with AD-like lesions from DNCB treatment. However, the levels were inverted when the AD-like mice were treated with BV using subcutaneous injection, and we observed that the AD symptoms were alleviated. BV is often used to treat AD but its mechanism has not been elucidated. Here, we suggest that BV alleviates AD through the inactivation of the complement system, especially by the induction of CD55.https://www.mdpi.com/2072-6651/11/5/239Bee venomcomplement systemdecay accelerating factoratopic dermatitiscomplement dependent cytotoxicitymembrane attack complex |
| spellingShingle | Yenny Kim Youn-Woo Lee Hangeun Kim Dae Kyun Chung Bee Venom Alleviates Atopic Dermatitis Symptoms through the Upregulation of Decay-Accelerating Factor (DAF/CD55) Toxins Bee venom complement system decay accelerating factor atopic dermatitis complement dependent cytotoxicity membrane attack complex |
| title | Bee Venom Alleviates Atopic Dermatitis Symptoms through the Upregulation of Decay-Accelerating Factor (DAF/CD55) |
| title_full | Bee Venom Alleviates Atopic Dermatitis Symptoms through the Upregulation of Decay-Accelerating Factor (DAF/CD55) |
| title_fullStr | Bee Venom Alleviates Atopic Dermatitis Symptoms through the Upregulation of Decay-Accelerating Factor (DAF/CD55) |
| title_full_unstemmed | Bee Venom Alleviates Atopic Dermatitis Symptoms through the Upregulation of Decay-Accelerating Factor (DAF/CD55) |
| title_short | Bee Venom Alleviates Atopic Dermatitis Symptoms through the Upregulation of Decay-Accelerating Factor (DAF/CD55) |
| title_sort | bee venom alleviates atopic dermatitis symptoms through the upregulation of decay accelerating factor daf cd55 |
| topic | Bee venom complement system decay accelerating factor atopic dermatitis complement dependent cytotoxicity membrane attack complex |
| url | https://www.mdpi.com/2072-6651/11/5/239 |
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