Generation of human GAPDH knock-in reporter iPSC lines for stable expression of tdTomato in pluripotent and differentiated culture conditions
Human induced pluripotent stem cells (iPSCs) can differentiate into multiple cell types and are utilized for research on human development and regenerative medicine. Here, we report the establishment of human GAPDH knock-in reporter iPSC lines (GAPDH-tdT1 and 2), via CRISPR/Cas9-mediated homologous...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-04-01
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| Series: | Stem Cell Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506122000538 |
| _version_ | 1818338516201897984 |
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| author | Daisuke Kamiya Teruyoshi Yamashita Teppei Akaboshi Yoshiki Yamaguchi Yayoi Toyooka Makoto Ikeya |
| author_facet | Daisuke Kamiya Teruyoshi Yamashita Teppei Akaboshi Yoshiki Yamaguchi Yayoi Toyooka Makoto Ikeya |
| author_sort | Daisuke Kamiya |
| collection | DOAJ |
| description | Human induced pluripotent stem cells (iPSCs) can differentiate into multiple cell types and are utilized for research on human development and regenerative medicine. Here, we report the establishment of human GAPDH knock-in reporter iPSC lines (GAPDH-tdT1 and 2), via CRISPR/Cas9-mediated homologous recombination, that stably express tdTomato as a constitutive cell label in both iPSCs and their differentiated derivatives. These cell lines will provide useful tools to trace cell locations and fates in 2D cultures and 3D organoids and will facilitate in vivo experiments. |
| first_indexed | 2024-12-13T15:12:21Z |
| format | Article |
| id | doaj.art-f8781a8643134b0c992ea156e210162e |
| institution | Directory Open Access Journal |
| issn | 1873-5061 |
| language | English |
| last_indexed | 2024-12-13T15:12:21Z |
| publishDate | 2022-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj.art-f8781a8643134b0c992ea156e210162e2022-12-21T23:40:50ZengElsevierStem Cell Research1873-50612022-04-0160102704Generation of human GAPDH knock-in reporter iPSC lines for stable expression of tdTomato in pluripotent and differentiated culture conditionsDaisuke Kamiya0Teruyoshi Yamashita1Teppei Akaboshi2Yoshiki Yamaguchi3Yayoi Toyooka4Makoto Ikeya5Department of Clinical Application, Center for iPS Cell Research and Application (CiRA), Kyoto University, Sakyo-ku, Kyoto 606-8507, Japan; Takeda-CiRA Joint Program (T-CiRA), Fujisawa, Kanagawa, Japan; Corresponding author at: Department of Clinical Application, Center for iPS Cell Research and Application (CiRA), Kyoto University, 26-1 Muraoka-Higashi 2-chome, Fujisawa, Kanagawa 251-8555, Japan.T-CiRA Discovery, Takeda Pharmaceutical Co. Ltd, Fujisawa, Kanagawa, Japan; Takeda-CiRA Joint Program (T-CiRA), Fujisawa, Kanagawa, JapanDepartment of Clinical Application, Center for iPS Cell Research and Application (CiRA), Kyoto University, Sakyo-ku, Kyoto 606-8507, Japan; Takeda-CiRA Joint Program (T-CiRA), Fujisawa, Kanagawa, JapanOrizuru Therapeutics Inc, Fujisawa, Kanagawa, JapanDepartment of Clinical Application, Center for iPS Cell Research and Application (CiRA), Kyoto University, Sakyo-ku, Kyoto 606-8507, Japan; Takeda-CiRA Joint Program (T-CiRA), Fujisawa, Kanagawa, JapanDepartment of Clinical Application, Center for iPS Cell Research and Application (CiRA), Kyoto University, Sakyo-ku, Kyoto 606-8507, Japan; Takeda-CiRA Joint Program (T-CiRA), Fujisawa, Kanagawa, JapanHuman induced pluripotent stem cells (iPSCs) can differentiate into multiple cell types and are utilized for research on human development and regenerative medicine. Here, we report the establishment of human GAPDH knock-in reporter iPSC lines (GAPDH-tdT1 and 2), via CRISPR/Cas9-mediated homologous recombination, that stably express tdTomato as a constitutive cell label in both iPSCs and their differentiated derivatives. These cell lines will provide useful tools to trace cell locations and fates in 2D cultures and 3D organoids and will facilitate in vivo experiments.http://www.sciencedirect.com/science/article/pii/S1873506122000538 |
| spellingShingle | Daisuke Kamiya Teruyoshi Yamashita Teppei Akaboshi Yoshiki Yamaguchi Yayoi Toyooka Makoto Ikeya Generation of human GAPDH knock-in reporter iPSC lines for stable expression of tdTomato in pluripotent and differentiated culture conditions Stem Cell Research |
| title | Generation of human GAPDH knock-in reporter iPSC lines for stable expression of tdTomato in pluripotent and differentiated culture conditions |
| title_full | Generation of human GAPDH knock-in reporter iPSC lines for stable expression of tdTomato in pluripotent and differentiated culture conditions |
| title_fullStr | Generation of human GAPDH knock-in reporter iPSC lines for stable expression of tdTomato in pluripotent and differentiated culture conditions |
| title_full_unstemmed | Generation of human GAPDH knock-in reporter iPSC lines for stable expression of tdTomato in pluripotent and differentiated culture conditions |
| title_short | Generation of human GAPDH knock-in reporter iPSC lines for stable expression of tdTomato in pluripotent and differentiated culture conditions |
| title_sort | generation of human gapdh knock in reporter ipsc lines for stable expression of tdtomato in pluripotent and differentiated culture conditions |
| url | http://www.sciencedirect.com/science/article/pii/S1873506122000538 |
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