Generation of human GAPDH knock-in reporter iPSC lines for stable expression of tdTomato in pluripotent and differentiated culture conditions

Generation of human GAPDH knock-in reporter iPSC lines for stable expression of tdTomato in pluripotent and differentiated culture conditions

Human induced pluripotent stem cells (iPSCs) can differentiate into multiple cell types and are utilized for research on human development and regenerative medicine. Here, we report the establishment of human GAPDH knock-in reporter iPSC lines (GAPDH-tdT1 and 2), via CRISPR/Cas9-mediated homologous...

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Bibliographic Details
Main Authors: Daisuke Kamiya, Teruyoshi Yamashita, Teppei Akaboshi, Yoshiki Yamaguchi, Yayoi Toyooka, Makoto Ikeya
Format: Article
Language:English
Published: Elsevier 2022-04-01
Series:Stem Cell Research
Online Access:http://www.sciencedirect.com/science/article/pii/S1873506122000538

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