Purification and biochemical characterization of four iron superoxide dismutases in Trypanosoma cruzi
Four superoxide dismutase (SOD) activities (SOD I, II, III, and IV) have been characterized in the epimastigote form of Trypanosoma cruzi. The total extract was subjected to two successive ammonium sulphate additions between 35 and 85%, and the resulting fraction was purified using two continuous ch...
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2008-05-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762008000300008 |
| _version_ | 1797757219238838272 |
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| author | Héctor Mateo Clotilde Marín Gregorio Pérez-Cordón Manuel Sánchez-Moreno |
| author_facet | Héctor Mateo Clotilde Marín Gregorio Pérez-Cordón Manuel Sánchez-Moreno |
| author_sort | Héctor Mateo |
| collection | DOAJ |
| description | Four superoxide dismutase (SOD) activities (SOD I, II, III, and IV) have been characterized in the epimastigote form of Trypanosoma cruzi. The total extract was subjected to two successive ammonium sulphate additions between 35 and 85%, and the resulting fraction was purified using two continuous chromatography processes (ion exchange and filtration). Enzymes were insensitive to cyanide but sensitive to hydrogen peroxide, properties characteristic of iron-containing SODs. The molecular masses of the different SODs were 20 kDa (SOD I), 60 kDa (SOD II), 50 kDa (SOD III) and 25 kDa (SOD IV), whereas the isoelectric points were 6.9, 6.8, 5.2 and 3.8, respectively. Subcellular location and digitonin experiments have shown that these SODs are mainly cytosolic, with small amounts in the low-mass organelles (SOD II and SOD I) and the mitochondrion (SOD III), where these enzymes play an important role in minimizing oxidative damage. |
| first_indexed | 2024-03-12T18:12:49Z |
| format | Article |
| id | doaj.art-f87b312b030f4c1ca390124495f21763 |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T18:12:49Z |
| publishDate | 2008-05-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-f87b312b030f4c1ca390124495f217632023-08-02T09:15:10ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80602008-05-01103327127610.1590/S0074-02762008000300008Purification and biochemical characterization of four iron superoxide dismutases in Trypanosoma cruziHéctor MateoClotilde MarínGregorio Pérez-CordónManuel Sánchez-MorenoFour superoxide dismutase (SOD) activities (SOD I, II, III, and IV) have been characterized in the epimastigote form of Trypanosoma cruzi. The total extract was subjected to two successive ammonium sulphate additions between 35 and 85%, and the resulting fraction was purified using two continuous chromatography processes (ion exchange and filtration). Enzymes were insensitive to cyanide but sensitive to hydrogen peroxide, properties characteristic of iron-containing SODs. The molecular masses of the different SODs were 20 kDa (SOD I), 60 kDa (SOD II), 50 kDa (SOD III) and 25 kDa (SOD IV), whereas the isoelectric points were 6.9, 6.8, 5.2 and 3.8, respectively. Subcellular location and digitonin experiments have shown that these SODs are mainly cytosolic, with small amounts in the low-mass organelles (SOD II and SOD I) and the mitochondrion (SOD III), where these enzymes play an important role in minimizing oxidative damage.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762008000300008superoxide dismutaseT. cruzichromatographysubcellular localization |
| spellingShingle | Héctor Mateo Clotilde Marín Gregorio Pérez-Cordón Manuel Sánchez-Moreno Purification and biochemical characterization of four iron superoxide dismutases in Trypanosoma cruzi Memorias do Instituto Oswaldo Cruz superoxide dismutase T. cruzi chromatography subcellular localization |
| title | Purification and biochemical characterization of four iron superoxide dismutases in Trypanosoma cruzi |
| title_full | Purification and biochemical characterization of four iron superoxide dismutases in Trypanosoma cruzi |
| title_fullStr | Purification and biochemical characterization of four iron superoxide dismutases in Trypanosoma cruzi |
| title_full_unstemmed | Purification and biochemical characterization of four iron superoxide dismutases in Trypanosoma cruzi |
| title_short | Purification and biochemical characterization of four iron superoxide dismutases in Trypanosoma cruzi |
| title_sort | purification and biochemical characterization of four iron superoxide dismutases in trypanosoma cruzi |
| topic | superoxide dismutase T. cruzi chromatography subcellular localization |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762008000300008 |
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