Inhibitory effect of zinc oxide nanoparticles and fibrillar chitosan‐zinc oxide nanostructures against herpes simplex virus infection
Abstract Although zinc ions have a strong antiviral impact against viral infections, they have a high level of toxicity in cell culture and in animal models. In the current work, ZnO nanoparticles (ZnO NPs) and fibrillar chitosan‐ZnO nanostructures (CS‐ZnO NSs) were synthesized in order to evaluate...
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| Format: | Article |
| Language: | English |
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Wiley
2023-06-01
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| Series: | The Journal of Engineering |
| Subjects: | |
| Online Access: | https://doi.org/10.1049/tje2.12268 |
| _version_ | 1797794600600993792 |
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| author | Mahya Abbasi Zahra Arab‐Bafrani Erfan Zabihi Amir Babaei Seyyed Mahdi Jafari Mahmood Barani Elham Mousavi |
| author_facet | Mahya Abbasi Zahra Arab‐Bafrani Erfan Zabihi Amir Babaei Seyyed Mahdi Jafari Mahmood Barani Elham Mousavi |
| author_sort | Mahya Abbasi |
| collection | DOAJ |
| description | Abstract Although zinc ions have a strong antiviral impact against viral infections, they have a high level of toxicity in cell culture and in animal models. In the current work, ZnO nanoparticles (ZnO NPs) and fibrillar chitosan‐ZnO nanostructures (CS‐ZnO NSs) were synthesized in order to evaluate their cytotoxicity and inhibitory impact against HSV (herpes simplex virus) replication in vitro using the MTT, plaque, and Real‐time PCR methods. Also, different characterization approaches such as FE‐SEM, XRD, FTIR and fluorescence microscope were used to evaluate prepared NPs. The findings revealed that CS‐ZnO NSs exhibit negligible cytotoxicity in cells, whereas ZnO NPs were exceedingly harmful at high doses. Also, CS‐ZnO NSs could considerably internalize cells and reduce virus titration in HSV‐infected cells. Furthermore, their inhibitory effect against HSV replication was quite effective, which may be more related to the antiviral activity of chitosan and ZnO. In conclusion, the new fibrillar chitosan‐ZnO NS was prepared with the least toxicity for host cells. |
| first_indexed | 2024-03-13T03:05:16Z |
| format | Article |
| id | doaj.art-f87c37aa98614cb68be9f5fe2cbabcff |
| institution | Directory Open Access Journal |
| issn | 2051-3305 |
| language | English |
| last_indexed | 2024-03-13T03:05:16Z |
| publishDate | 2023-06-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Journal of Engineering |
| spelling | doaj.art-f87c37aa98614cb68be9f5fe2cbabcff2023-06-27T07:44:34ZengWileyThe Journal of Engineering2051-33052023-06-0120236n/an/a10.1049/tje2.12268Inhibitory effect of zinc oxide nanoparticles and fibrillar chitosan‐zinc oxide nanostructures against herpes simplex virus infectionMahya Abbasi0Zahra Arab‐Bafrani1Erfan Zabihi2Amir Babaei3Seyyed Mahdi Jafari4Mahmood Barani5Elham Mousavi6Department of Medical Microbiology (Bacteriology and virology) Afzalipour Faculty of Medicine Kerman University of Medical Sciences Kerman IranMetabolic Disorders Research Center Golestan University of Medical Sciences Gorgan IranDepartment of Polymer Engineering Faculty of Engineering Golestan University Gorgan IranDepartment of Polymer Engineering Faculty of Engineering Golestan University Gorgan IranNutrition and Bromatology Group, Department of Analytical Chemistry and Food Science, Faculty of Science Universidade de Vigo Qurense SpainMedical Mycology and Bacteriology Research Center Kerman University of Medical Sciences Kerman IranMedical Mycology and Bacteriology Research Center Kerman University of Medical Sciences Kerman IranAbstract Although zinc ions have a strong antiviral impact against viral infections, they have a high level of toxicity in cell culture and in animal models. In the current work, ZnO nanoparticles (ZnO NPs) and fibrillar chitosan‐ZnO nanostructures (CS‐ZnO NSs) were synthesized in order to evaluate their cytotoxicity and inhibitory impact against HSV (herpes simplex virus) replication in vitro using the MTT, plaque, and Real‐time PCR methods. Also, different characterization approaches such as FE‐SEM, XRD, FTIR and fluorescence microscope were used to evaluate prepared NPs. The findings revealed that CS‐ZnO NSs exhibit negligible cytotoxicity in cells, whereas ZnO NPs were exceedingly harmful at high doses. Also, CS‐ZnO NSs could considerably internalize cells and reduce virus titration in HSV‐infected cells. Furthermore, their inhibitory effect against HSV replication was quite effective, which may be more related to the antiviral activity of chitosan and ZnO. In conclusion, the new fibrillar chitosan‐ZnO NS was prepared with the least toxicity for host cells.https://doi.org/10.1049/tje2.12268fibrillar chitosanherpes simplex virusmicroscopy assayszinc oxide nanoparticles |
| spellingShingle | Mahya Abbasi Zahra Arab‐Bafrani Erfan Zabihi Amir Babaei Seyyed Mahdi Jafari Mahmood Barani Elham Mousavi Inhibitory effect of zinc oxide nanoparticles and fibrillar chitosan‐zinc oxide nanostructures against herpes simplex virus infection The Journal of Engineering fibrillar chitosan herpes simplex virus microscopy assays zinc oxide nanoparticles |
| title | Inhibitory effect of zinc oxide nanoparticles and fibrillar chitosan‐zinc oxide nanostructures against herpes simplex virus infection |
| title_full | Inhibitory effect of zinc oxide nanoparticles and fibrillar chitosan‐zinc oxide nanostructures against herpes simplex virus infection |
| title_fullStr | Inhibitory effect of zinc oxide nanoparticles and fibrillar chitosan‐zinc oxide nanostructures against herpes simplex virus infection |
| title_full_unstemmed | Inhibitory effect of zinc oxide nanoparticles and fibrillar chitosan‐zinc oxide nanostructures against herpes simplex virus infection |
| title_short | Inhibitory effect of zinc oxide nanoparticles and fibrillar chitosan‐zinc oxide nanostructures against herpes simplex virus infection |
| title_sort | inhibitory effect of zinc oxide nanoparticles and fibrillar chitosan zinc oxide nanostructures against herpes simplex virus infection |
| topic | fibrillar chitosan herpes simplex virus microscopy assays zinc oxide nanoparticles |
| url | https://doi.org/10.1049/tje2.12268 |
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