Antioxidant potentials of furanodihydrobenzoxanthones from Artocarpus elasticus and their protection against oxLDL induced injury in SH-SY5Y cells
Exposure to reactive oxygen species (ROS) leads to the oxidation of low-density lipoproteins (LDL), converting them into oxidized ones (oxLDL), which are involved in the pathogenesis of Alzheimer's disease, suggesting a potential link between lipid dysregulation and neurodegenerative processes....
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| Format: | Article |
| Language: | English |
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Elsevier
2023-09-01
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| Series: | Biomedicine & Pharmacotherapy |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0753332223010697 |
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| author | Aizhamal Baiseitova Abdul Bari Shah Abdul Majid Khan Muhammad Idrees Jeong Ho Kim Yong Hyun Lee Il-Keun Kong Ki Hun Park |
| author_facet | Aizhamal Baiseitova Abdul Bari Shah Abdul Majid Khan Muhammad Idrees Jeong Ho Kim Yong Hyun Lee Il-Keun Kong Ki Hun Park |
| author_sort | Aizhamal Baiseitova |
| collection | DOAJ |
| description | Exposure to reactive oxygen species (ROS) leads to the oxidation of low-density lipoproteins (LDL), converting them into oxidized ones (oxLDL), which are involved in the pathogenesis of Alzheimer's disease, suggesting a potential link between lipid dysregulation and neurodegenerative processes. Phenolic metabolites derived from Artocarpus elasticus root bark were found to possess significant antioxidant properties at three different radical scavenging assays, including 2,2-diphenyl-1-picrylhydrazyl (DPPH), oxygen radical absorbance capacity (ORAC), and thiobarbituric acid reactive substances (TBARS). Among them, furanodihydrobenzoxanthones (1-3) demonstrated notable protection against Cu2+ induced LDL oxidation, with IC50 values ranging from 0.9 to 2.9 μM in measurement of the malondialdehyde (MDA) production at TBARS and prolonged lag times (>180 min) in the generation of conjugated diene (CD). At a concentration of 10 μM, all three compounds (1-3) effectively protected against LDL oxidation as determined by relative electrophoretic mobility (REM). The most potent compound 1 defended human neuroblastoma SH-SY5Y cells from oxLDL-mediated dysfunction, including oxLDL-induced cytotoxicity, inhibited reactive oxygen species (ROS) formation, and enhancing mitochondrial membrane potential (ΔΨm). Individual components annotation in the ethylacetate extract was performed using LC-ESI-QTOF/MS, which serves as a chemotaxonomic marker for A. elasticus root barks. |
| first_indexed | 2024-03-12T15:04:56Z |
| format | Article |
| id | doaj.art-f87d8c5389ff411ba413e3e9fd1bb4c9 |
| institution | Directory Open Access Journal |
| issn | 0753-3322 |
| language | English |
| last_indexed | 2024-03-12T15:04:56Z |
| publishDate | 2023-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biomedicine & Pharmacotherapy |
| spelling | doaj.art-f87d8c5389ff411ba413e3e9fd1bb4c92023-08-13T04:53:17ZengElsevierBiomedicine & Pharmacotherapy0753-33222023-09-01165115278Antioxidant potentials of furanodihydrobenzoxanthones from Artocarpus elasticus and their protection against oxLDL induced injury in SH-SY5Y cellsAizhamal Baiseitova0Abdul Bari Shah1Abdul Majid Khan2Muhammad Idrees3Jeong Ho Kim4Yong Hyun Lee5Il-Keun Kong6Ki Hun Park7Division of Applied Life Science (BK21 Four), IALS, Gyeongsang National University, Jinju 52828, Republic of KoreaDivision of Applied Life Science (BK21 Four), IALS, Gyeongsang National University, Jinju 52828, Republic of KoreaDivision of Applied Life Science (BK21 Four), IALS, Gyeongsang National University, Jinju 52828, Republic of KoreaDivision of Applied Life Science (BK21 Four), IALS, Gyeongsang National University, Jinju 52828, Republic of KoreaDivision of Applied Life Science (BK21 Four), IALS, Gyeongsang National University, Jinju 52828, Republic of KoreaDivision of Applied Life Science (BK21 Four), IALS, Gyeongsang National University, Jinju 52828, Republic of KoreaDivision of Applied Life Science (BK21 Four), IALS, Gyeongsang National University, Jinju 52828, Republic of KoreaCorresponding author.; Division of Applied Life Science (BK21 Four), IALS, Gyeongsang National University, Jinju 52828, Republic of KoreaExposure to reactive oxygen species (ROS) leads to the oxidation of low-density lipoproteins (LDL), converting them into oxidized ones (oxLDL), which are involved in the pathogenesis of Alzheimer's disease, suggesting a potential link between lipid dysregulation and neurodegenerative processes. Phenolic metabolites derived from Artocarpus elasticus root bark were found to possess significant antioxidant properties at three different radical scavenging assays, including 2,2-diphenyl-1-picrylhydrazyl (DPPH), oxygen radical absorbance capacity (ORAC), and thiobarbituric acid reactive substances (TBARS). Among them, furanodihydrobenzoxanthones (1-3) demonstrated notable protection against Cu2+ induced LDL oxidation, with IC50 values ranging from 0.9 to 2.9 μM in measurement of the malondialdehyde (MDA) production at TBARS and prolonged lag times (>180 min) in the generation of conjugated diene (CD). At a concentration of 10 μM, all three compounds (1-3) effectively protected against LDL oxidation as determined by relative electrophoretic mobility (REM). The most potent compound 1 defended human neuroblastoma SH-SY5Y cells from oxLDL-mediated dysfunction, including oxLDL-induced cytotoxicity, inhibited reactive oxygen species (ROS) formation, and enhancing mitochondrial membrane potential (ΔΨm). Individual components annotation in the ethylacetate extract was performed using LC-ESI-QTOF/MS, which serves as a chemotaxonomic marker for A. elasticus root barks.http://www.sciencedirect.com/science/article/pii/S0753332223010697Artocarpus elasticusFuranodihydrobenzoxanthoneAntioxidantLDL oxidationHuman neuroblastoma SH-SY5Y cells |
| spellingShingle | Aizhamal Baiseitova Abdul Bari Shah Abdul Majid Khan Muhammad Idrees Jeong Ho Kim Yong Hyun Lee Il-Keun Kong Ki Hun Park Antioxidant potentials of furanodihydrobenzoxanthones from Artocarpus elasticus and their protection against oxLDL induced injury in SH-SY5Y cells Biomedicine & Pharmacotherapy Artocarpus elasticus Furanodihydrobenzoxanthone Antioxidant LDL oxidation Human neuroblastoma SH-SY5Y cells |
| title | Antioxidant potentials of furanodihydrobenzoxanthones from Artocarpus elasticus and their protection against oxLDL induced injury in SH-SY5Y cells |
| title_full | Antioxidant potentials of furanodihydrobenzoxanthones from Artocarpus elasticus and their protection against oxLDL induced injury in SH-SY5Y cells |
| title_fullStr | Antioxidant potentials of furanodihydrobenzoxanthones from Artocarpus elasticus and their protection against oxLDL induced injury in SH-SY5Y cells |
| title_full_unstemmed | Antioxidant potentials of furanodihydrobenzoxanthones from Artocarpus elasticus and their protection against oxLDL induced injury in SH-SY5Y cells |
| title_short | Antioxidant potentials of furanodihydrobenzoxanthones from Artocarpus elasticus and their protection against oxLDL induced injury in SH-SY5Y cells |
| title_sort | antioxidant potentials of furanodihydrobenzoxanthones from artocarpus elasticus and their protection against oxldl induced injury in sh sy5y cells |
| topic | Artocarpus elasticus Furanodihydrobenzoxanthone Antioxidant LDL oxidation Human neuroblastoma SH-SY5Y cells |
| url | http://www.sciencedirect.com/science/article/pii/S0753332223010697 |
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