Stable Isotope Ratio Analysis for Authentication of Natural Antioxidant Cannabidiol (CBD) from <i>Cannabis sativa</i>
Cannabidiol (CBD) is a non-psychoactive cannabinoid of <i>Cannabis sativa</i> that exhibits several beneficial pharmacological effects, including anti-inflammatory and antioxidant properties. The molecule can be obtained via extraction from the plant or through a biosynthetic route. The...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-07-01
|
| Series: | Antioxidants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-3921/12/7/1421 |
| _version_ | 1797590550304522240 |
|---|---|
| author | Matteo Perini Alessio Gaggiotti Silvia Pianezze Luca Ziller Roberto Larcher |
| author_facet | Matteo Perini Alessio Gaggiotti Silvia Pianezze Luca Ziller Roberto Larcher |
| author_sort | Matteo Perini |
| collection | DOAJ |
| description | Cannabidiol (CBD) is a non-psychoactive cannabinoid of <i>Cannabis sativa</i> that exhibits several beneficial pharmacological effects, including anti-inflammatory and antioxidant properties. The molecule can be obtained via extraction from the plant or through a biosynthetic route. The two products have both advantages and disadvantages, thus necessitating the development of methods capable of distinguishing between the two products. In this study, for the first time, the analysis of the stable isotope ratios of oxygen and hydrogen demonstrated high efficiency in the discrimination of CBD of a totally natural origin from that obtained through chemical synthesis. Considering a probability level of 95%, it was possible to identify threshold values for <i>δ</i><sup>2</sup>H and <i>δ</i><sup>18</sup>O of the totally natural CBD of −215‰ and +23.4‰, respectively. Higher values may indicate a non-entirely natural origin of CBD (i.e., a biosynthetic molecule). |
| first_indexed | 2024-03-11T01:22:07Z |
| format | Article |
| id | doaj.art-f87f5078cd364370b1bfd762bb7dd899 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-11T01:22:07Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-f87f5078cd364370b1bfd762bb7dd8992023-11-18T18:05:43ZengMDPI AGAntioxidants2076-39212023-07-01127142110.3390/antiox12071421Stable Isotope Ratio Analysis for Authentication of Natural Antioxidant Cannabidiol (CBD) from <i>Cannabis sativa</i>Matteo Perini0Alessio Gaggiotti1Silvia Pianezze2Luca Ziller3Roberto Larcher4Fondazione Edmund Mach, Via E. Mach 1, 38098 San Michele all’Adige, ItalyFarmech, Piazza Duomo 20, 20122 Milano, ItalyFondazione Edmund Mach, Via E. Mach 1, 38098 San Michele all’Adige, ItalyFondazione Edmund Mach, Via E. Mach 1, 38098 San Michele all’Adige, ItalyFondazione Edmund Mach, Via E. Mach 1, 38098 San Michele all’Adige, ItalyCannabidiol (CBD) is a non-psychoactive cannabinoid of <i>Cannabis sativa</i> that exhibits several beneficial pharmacological effects, including anti-inflammatory and antioxidant properties. The molecule can be obtained via extraction from the plant or through a biosynthetic route. The two products have both advantages and disadvantages, thus necessitating the development of methods capable of distinguishing between the two products. In this study, for the first time, the analysis of the stable isotope ratios of oxygen and hydrogen demonstrated high efficiency in the discrimination of CBD of a totally natural origin from that obtained through chemical synthesis. Considering a probability level of 95%, it was possible to identify threshold values for <i>δ</i><sup>2</sup>H and <i>δ</i><sup>18</sup>O of the totally natural CBD of −215‰ and +23.4‰, respectively. Higher values may indicate a non-entirely natural origin of CBD (i.e., a biosynthetic molecule).https://www.mdpi.com/2076-3921/12/7/1421cannabidiol<i>Cannabis sativa</i>stable isotope ratio analysisbiosyntheticauthentication |
| spellingShingle | Matteo Perini Alessio Gaggiotti Silvia Pianezze Luca Ziller Roberto Larcher Stable Isotope Ratio Analysis for Authentication of Natural Antioxidant Cannabidiol (CBD) from <i>Cannabis sativa</i> Antioxidants cannabidiol <i>Cannabis sativa</i> stable isotope ratio analysis biosynthetic authentication |
| title | Stable Isotope Ratio Analysis for Authentication of Natural Antioxidant Cannabidiol (CBD) from <i>Cannabis sativa</i> |
| title_full | Stable Isotope Ratio Analysis for Authentication of Natural Antioxidant Cannabidiol (CBD) from <i>Cannabis sativa</i> |
| title_fullStr | Stable Isotope Ratio Analysis for Authentication of Natural Antioxidant Cannabidiol (CBD) from <i>Cannabis sativa</i> |
| title_full_unstemmed | Stable Isotope Ratio Analysis for Authentication of Natural Antioxidant Cannabidiol (CBD) from <i>Cannabis sativa</i> |
| title_short | Stable Isotope Ratio Analysis for Authentication of Natural Antioxidant Cannabidiol (CBD) from <i>Cannabis sativa</i> |
| title_sort | stable isotope ratio analysis for authentication of natural antioxidant cannabidiol cbd from i cannabis sativa i |
| topic | cannabidiol <i>Cannabis sativa</i> stable isotope ratio analysis biosynthetic authentication |
| url | https://www.mdpi.com/2076-3921/12/7/1421 |
| work_keys_str_mv | AT matteoperini stableisotoperatioanalysisforauthenticationofnaturalantioxidantcannabidiolcbdfromicannabissativai AT alessiogaggiotti stableisotoperatioanalysisforauthenticationofnaturalantioxidantcannabidiolcbdfromicannabissativai AT silviapianezze stableisotoperatioanalysisforauthenticationofnaturalantioxidantcannabidiolcbdfromicannabissativai AT lucaziller stableisotoperatioanalysisforauthenticationofnaturalantioxidantcannabidiolcbdfromicannabissativai AT robertolarcher stableisotoperatioanalysisforauthenticationofnaturalantioxidantcannabidiolcbdfromicannabissativai |