Expression of interleukin 1, interleukin 27, and TNF α genes in patients with ischemic cardiomyopathy versus idiopathic dilated cardiomyopathy: A case‐control study
Abstract Background and Aims Congestive heart failure is a complex multifactorial syndrome due to tissue hypoperfusion that is affected by some factors like inflammatory cytokines. In our study, we investigated the exact gene expression of three inflammatory cytokines in ischemic and idiopathic card...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2022-07-01
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| Series: | Health Science Reports |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/hsr2.701 |
| _version_ | 1811308479691882496 |
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| author | Mahdiyar Iravani Saadi Javad Salami Hanieh Abdi Nadiya Kheradmand Ehsan Nabi Abdolyousefi Mahmoud Torkamani Zahed Karimi Shahram Agah Zahra Rahimian Alireza Manafi |
| author_facet | Mahdiyar Iravani Saadi Javad Salami Hanieh Abdi Nadiya Kheradmand Ehsan Nabi Abdolyousefi Mahmoud Torkamani Zahed Karimi Shahram Agah Zahra Rahimian Alireza Manafi |
| author_sort | Mahdiyar Iravani Saadi |
| collection | DOAJ |
| description | Abstract Background and Aims Congestive heart failure is a complex multifactorial syndrome due to tissue hypoperfusion that is affected by some factors like inflammatory cytokines. In our study, we investigated the exact gene expression of three inflammatory cytokines in ischemic and idiopathic cardiomyopathy patients. Methods From 49 studied recipients in the ischemic group, 23 (46.9%) were male and from 40 studied recipients in the idiopathic dilated cardiomyopathy group, 19 (47.5%) were male. For the quantitative analysis of interleukin (IL)‐1, IL‐27, and tumor necrosis factor (TNF)‐α messenger RNAs expression level, the SYBR Green real‐time polymerase chain reaction method was performed using SYBRPremix Ex TaqTM II (Tli RNaseH Plus; Takara) and designed primers specific for each gene in an iQ5 thermocycler (BioRad Laboratories) according to the manufacturer's instructions. Results Our results showed that the expression level of IL‐1 and TNF‐α were significantly higher in the ischemic patients compared to healthy controls (p < 0.001, p < 0.01, respectively); also, we found higher levels of IL‐1 and IL‐27 gene expressions in idiopathic patients compared to healthy controls (p < 0.001, p < 0.001, respectively). There were not any significant differences in IL‐1, IL‐27, and TNF‐α expression levels between ischemic patients and idiopathic ones. Conclusion Although we would introduce IL‐1, IL‐27, and TNF‐α as effective inflammatory cytokines on myocardial functions in ischemic and idiopathic cardiomyopathy patients, there is not any difference between these two groups in gene expression of three main inflammatory cytokines. |
| first_indexed | 2024-04-13T09:23:54Z |
| format | Article |
| id | doaj.art-f884ba618b674a3291aa38b06ce08719 |
| institution | Directory Open Access Journal |
| issn | 2398-8835 |
| language | English |
| last_indexed | 2024-04-13T09:23:54Z |
| publishDate | 2022-07-01 |
| publisher | Wiley |
| record_format | Article |
| series | Health Science Reports |
| spelling | doaj.art-f884ba618b674a3291aa38b06ce087192022-12-22T02:52:31ZengWileyHealth Science Reports2398-88352022-07-0154n/an/a10.1002/hsr2.701Expression of interleukin 1, interleukin 27, and TNF α genes in patients with ischemic cardiomyopathy versus idiopathic dilated cardiomyopathy: A case‐control studyMahdiyar Iravani Saadi0Javad Salami1Hanieh Abdi2Nadiya Kheradmand3Ehsan Nabi Abdolyousefi4Mahmoud Torkamani5Zahed Karimi6Shahram Agah7Zahra Rahimian8Alireza Manafi9Colorectal Research Center Iran University of Medical Sciences Tehran IranDepartment of Nursing Shiraz University of Medical Sciences Shiraz IranStudent Research Committee Shiraz University of Medical Sciences Shiraz IranColorectal Research Center Iran University of Medical Sciences Tehran IranColorectal Research Center Iran University of Medical Sciences Tehran IranColorectal Research Center Iran University of Medical Sciences Tehran IranColorectal Research Center Iran University of Medical Sciences Tehran IranColorectal Research Center Iran University of Medical Sciences Tehran IranStudent Research Committee Shiraz University of Medical Sciences Shiraz IranStudent Research Committee Shiraz University of Medical Sciences Shiraz IranAbstract Background and Aims Congestive heart failure is a complex multifactorial syndrome due to tissue hypoperfusion that is affected by some factors like inflammatory cytokines. In our study, we investigated the exact gene expression of three inflammatory cytokines in ischemic and idiopathic cardiomyopathy patients. Methods From 49 studied recipients in the ischemic group, 23 (46.9%) were male and from 40 studied recipients in the idiopathic dilated cardiomyopathy group, 19 (47.5%) were male. For the quantitative analysis of interleukin (IL)‐1, IL‐27, and tumor necrosis factor (TNF)‐α messenger RNAs expression level, the SYBR Green real‐time polymerase chain reaction method was performed using SYBRPremix Ex TaqTM II (Tli RNaseH Plus; Takara) and designed primers specific for each gene in an iQ5 thermocycler (BioRad Laboratories) according to the manufacturer's instructions. Results Our results showed that the expression level of IL‐1 and TNF‐α were significantly higher in the ischemic patients compared to healthy controls (p < 0.001, p < 0.01, respectively); also, we found higher levels of IL‐1 and IL‐27 gene expressions in idiopathic patients compared to healthy controls (p < 0.001, p < 0.001, respectively). There were not any significant differences in IL‐1, IL‐27, and TNF‐α expression levels between ischemic patients and idiopathic ones. Conclusion Although we would introduce IL‐1, IL‐27, and TNF‐α as effective inflammatory cytokines on myocardial functions in ischemic and idiopathic cardiomyopathy patients, there is not any difference between these two groups in gene expression of three main inflammatory cytokines.https://doi.org/10.1002/hsr2.701congestive heart failureidiopathic dilated cardiomyopathyinterleukinischemic cardiomyopathy |
| spellingShingle | Mahdiyar Iravani Saadi Javad Salami Hanieh Abdi Nadiya Kheradmand Ehsan Nabi Abdolyousefi Mahmoud Torkamani Zahed Karimi Shahram Agah Zahra Rahimian Alireza Manafi Expression of interleukin 1, interleukin 27, and TNF α genes in patients with ischemic cardiomyopathy versus idiopathic dilated cardiomyopathy: A case‐control study Health Science Reports congestive heart failure idiopathic dilated cardiomyopathy interleukin ischemic cardiomyopathy |
| title | Expression of interleukin 1, interleukin 27, and TNF α genes in patients with ischemic cardiomyopathy versus idiopathic dilated cardiomyopathy: A case‐control study |
| title_full | Expression of interleukin 1, interleukin 27, and TNF α genes in patients with ischemic cardiomyopathy versus idiopathic dilated cardiomyopathy: A case‐control study |
| title_fullStr | Expression of interleukin 1, interleukin 27, and TNF α genes in patients with ischemic cardiomyopathy versus idiopathic dilated cardiomyopathy: A case‐control study |
| title_full_unstemmed | Expression of interleukin 1, interleukin 27, and TNF α genes in patients with ischemic cardiomyopathy versus idiopathic dilated cardiomyopathy: A case‐control study |
| title_short | Expression of interleukin 1, interleukin 27, and TNF α genes in patients with ischemic cardiomyopathy versus idiopathic dilated cardiomyopathy: A case‐control study |
| title_sort | expression of interleukin 1 interleukin 27 and tnf α genes in patients with ischemic cardiomyopathy versus idiopathic dilated cardiomyopathy a case control study |
| topic | congestive heart failure idiopathic dilated cardiomyopathy interleukin ischemic cardiomyopathy |
| url | https://doi.org/10.1002/hsr2.701 |
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