Grass carp SERPINA1 inhibits GCRV infection through degrading CF2
SERPINA1, a member of the serine protease inhibitor family, plays a role in viral infection and inflammation by regulating the activities of serine and cysteine proteases. To date, there have been no reports on the immune function of SERPINA1 in fishes. In this study, we first cloned the serpina1 ge...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2022.969517/full |
| _version_ | 1828112377356222464 |
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| author | Yangyang Li Yangyang Li Liangming Chen Liangming Chen Rong Huang Yangyu Li Yangyu Li Cheng Yang Bin Gui Bin Gui Yongming Li Lanjie Liao Zuoyan Zhu Yaping Wang Yaping Wang |
| author_facet | Yangyang Li Yangyang Li Liangming Chen Liangming Chen Rong Huang Yangyu Li Yangyu Li Cheng Yang Bin Gui Bin Gui Yongming Li Lanjie Liao Zuoyan Zhu Yaping Wang Yaping Wang |
| author_sort | Yangyang Li |
| collection | DOAJ |
| description | SERPINA1, a member of the serine protease inhibitor family, plays a role in viral infection and inflammation by regulating the activities of serine and cysteine proteases. To date, there have been no reports on the immune function of SERPINA1 in fishes. In this study, we first cloned the serpina1 gene of grass carp (Ctenopharyngodon idellus) and found that it could respond rapidly to the infection of Grass carp reovirus (GCRV), and overexpression of serpina1 could enhance the antiviral response of CIK cells. A polyclonal antibody of SERPINA1 was prepared, and the protein interacting with SERPINA1 was screened by CoIP/MS in grass carp hepatopancreas tissue. It was found that SERPINA1 interacted with coagulation factor 2 (CF2) and could degrade it in a dose-dependent manner. In addition, overexpression of cf2 contributed to the infection of GCRV in CIK cells, whereas co-expression of serpina1 and cf2 in grass carp reduced the copy number of GCRV in cells. The results showed that grass carp SERPINA1 could inhibit GCRV infection by degrading CF2. This study proposes that SERPINA1 can inhibit viral infection through interaction with the coagulation factor, providing new insights into the molecular mechanism of SERPINA1’s antiviral function. |
| first_indexed | 2024-04-11T11:48:16Z |
| format | Article |
| id | doaj.art-f8864507090f4053890ce886e7c106bf |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-04-11T11:48:16Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-f8864507090f4053890ce886e7c106bf2022-12-22T04:25:29ZengFrontiers Media S.A.Frontiers in Immunology1664-32242022-09-011310.3389/fimmu.2022.969517969517Grass carp SERPINA1 inhibits GCRV infection through degrading CF2Yangyang Li0Yangyang Li1Liangming Chen2Liangming Chen3Rong Huang4Yangyu Li5Yangyu Li6Cheng Yang7Bin Gui8Bin Gui9Yongming Li10Lanjie Liao11Zuoyan Zhu12Yaping Wang13Yaping Wang14State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaState Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaState Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, ChinaState Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaState Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, ChinaState Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaState Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, ChinaState Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, ChinaState Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, ChinaState Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, ChinaInnovative Academy of Seed Design, Chinese Academy of Sciences, Beijing, ChinaSERPINA1, a member of the serine protease inhibitor family, plays a role in viral infection and inflammation by regulating the activities of serine and cysteine proteases. To date, there have been no reports on the immune function of SERPINA1 in fishes. In this study, we first cloned the serpina1 gene of grass carp (Ctenopharyngodon idellus) and found that it could respond rapidly to the infection of Grass carp reovirus (GCRV), and overexpression of serpina1 could enhance the antiviral response of CIK cells. A polyclonal antibody of SERPINA1 was prepared, and the protein interacting with SERPINA1 was screened by CoIP/MS in grass carp hepatopancreas tissue. It was found that SERPINA1 interacted with coagulation factor 2 (CF2) and could degrade it in a dose-dependent manner. In addition, overexpression of cf2 contributed to the infection of GCRV in CIK cells, whereas co-expression of serpina1 and cf2 in grass carp reduced the copy number of GCRV in cells. The results showed that grass carp SERPINA1 could inhibit GCRV infection by degrading CF2. This study proposes that SERPINA1 can inhibit viral infection through interaction with the coagulation factor, providing new insights into the molecular mechanism of SERPINA1’s antiviral function.https://www.frontiersin.org/articles/10.3389/fimmu.2022.969517/fullSERPINA1CF2GCRVoverexpressionvirus suppression |
| spellingShingle | Yangyang Li Yangyang Li Liangming Chen Liangming Chen Rong Huang Yangyu Li Yangyu Li Cheng Yang Bin Gui Bin Gui Yongming Li Lanjie Liao Zuoyan Zhu Yaping Wang Yaping Wang Grass carp SERPINA1 inhibits GCRV infection through degrading CF2 Frontiers in Immunology SERPINA1 CF2 GCRV overexpression virus suppression |
| title | Grass carp SERPINA1 inhibits GCRV infection through degrading CF2 |
| title_full | Grass carp SERPINA1 inhibits GCRV infection through degrading CF2 |
| title_fullStr | Grass carp SERPINA1 inhibits GCRV infection through degrading CF2 |
| title_full_unstemmed | Grass carp SERPINA1 inhibits GCRV infection through degrading CF2 |
| title_short | Grass carp SERPINA1 inhibits GCRV infection through degrading CF2 |
| title_sort | grass carp serpina1 inhibits gcrv infection through degrading cf2 |
| topic | SERPINA1 CF2 GCRV overexpression virus suppression |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2022.969517/full |
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