ZmLEA3 enhanced low temperature stress tolerance by protecting the activity of enzyme in transgenic tobacco
Late embryogenesis abundant (LEA) proteins are a family of small highly hydrophilic proteins that accumulate at the onset of seed desiccation and in response to adverse conditions such as drought, salinity, low temperature or water deficit. In previous studies, we demonstrated that ZmLEA3 could enha...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2016-07-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fpls.2016.01011/full |
| _version_ | 1819198583576985600 |
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| author | Yang Liu Jianan Liang Liping Sun Dequan Li Xinghong Yang |
| author_facet | Yang Liu Jianan Liang Liping Sun Dequan Li Xinghong Yang |
| author_sort | Yang Liu |
| collection | DOAJ |
| description | Late embryogenesis abundant (LEA) proteins are a family of small highly hydrophilic proteins that accumulate at the onset of seed desiccation and in response to adverse conditions such as drought, salinity, low temperature or water deficit. In previous studies, we demonstrated that ZmLEA3 could enhance the transgenic tobacco tolerance to osmotic and oxidative stresses. Here, we demonstrated that the transcription of ZmLEA3 in the maize stems could be significantly induced by low temperature and osmotic stresses and by treatment with ABA and H2O2. Further study indicated that ZmLEA3 is a single copy gene in the maize genome. The ZmLEA3 protein could protect LDH activity at low temperatures. The overexpression of ZmLEA3 conferred tolerance to low temperature stress to transgenic tobacco, yeast (GS115) and E.coli (BL21). |
| first_indexed | 2024-12-23T03:02:45Z |
| format | Article |
| id | doaj.art-f887918d048f410aa3922b74b76cc3ca |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-23T03:02:45Z |
| publishDate | 2016-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-f887918d048f410aa3922b74b76cc3ca2022-12-21T18:02:24ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2016-07-01710.3389/fpls.2016.01011196756ZmLEA3 enhanced low temperature stress tolerance by protecting the activity of enzyme in transgenic tobaccoYang Liu0Jianan Liang1Liping Sun2Dequan Li3Xinghong Yang4Shandong Agricultural UniversityShandong Agricultural UniversityTaishan Medical UniversityShandong Agricultural UniversityShandong Agricultural UniversityLate embryogenesis abundant (LEA) proteins are a family of small highly hydrophilic proteins that accumulate at the onset of seed desiccation and in response to adverse conditions such as drought, salinity, low temperature or water deficit. In previous studies, we demonstrated that ZmLEA3 could enhance the transgenic tobacco tolerance to osmotic and oxidative stresses. Here, we demonstrated that the transcription of ZmLEA3 in the maize stems could be significantly induced by low temperature and osmotic stresses and by treatment with ABA and H2O2. Further study indicated that ZmLEA3 is a single copy gene in the maize genome. The ZmLEA3 protein could protect LDH activity at low temperatures. The overexpression of ZmLEA3 conferred tolerance to low temperature stress to transgenic tobacco, yeast (GS115) and E.coli (BL21).http://journal.frontiersin.org/Journal/10.3389/fpls.2016.01011/fullMaizelow temperature stressLEA proteinTransgenic geneZmLEA3 |
| spellingShingle | Yang Liu Jianan Liang Liping Sun Dequan Li Xinghong Yang ZmLEA3 enhanced low temperature stress tolerance by protecting the activity of enzyme in transgenic tobacco Frontiers in Plant Science Maize low temperature stress LEA protein Transgenic gene ZmLEA3 |
| title | ZmLEA3 enhanced low temperature stress tolerance by protecting the activity of enzyme in transgenic tobacco |
| title_full | ZmLEA3 enhanced low temperature stress tolerance by protecting the activity of enzyme in transgenic tobacco |
| title_fullStr | ZmLEA3 enhanced low temperature stress tolerance by protecting the activity of enzyme in transgenic tobacco |
| title_full_unstemmed | ZmLEA3 enhanced low temperature stress tolerance by protecting the activity of enzyme in transgenic tobacco |
| title_short | ZmLEA3 enhanced low temperature stress tolerance by protecting the activity of enzyme in transgenic tobacco |
| title_sort | zmlea3 enhanced low temperature stress tolerance by protecting the activity of enzyme in transgenic tobacco |
| topic | Maize low temperature stress LEA protein Transgenic gene ZmLEA3 |
| url | http://journal.frontiersin.org/Journal/10.3389/fpls.2016.01011/full |
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