A Parallel Comparison of Antigen Candidates for Development of an Optimized Serological Diagnosis of Schistosomiasis Japonica in the Philippines
Schistosoma japonicum is stubbornly persistent in China and the Philippines. Fast and accurate diagnostic tools are required to monitor effective control measures against schistosomiasis japonica. Promising antigen candidates for the serological diagnosis of schistosomiasis japonica have generally b...
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| Format: | Article |
| Language: | English |
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Elsevier
2017-10-01
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| Series: | EBioMedicine |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S235239641730364X |
| _version_ | 1818133850277019648 |
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| author | Pengfei Cai Kosala G. Weerakoon Yi Mu David U. Olveda Xianyu Piao Shuai Liu Remigio M. Olveda Qijun Chen Allen G. Ross Donald P. McManus |
| author_facet | Pengfei Cai Kosala G. Weerakoon Yi Mu David U. Olveda Xianyu Piao Shuai Liu Remigio M. Olveda Qijun Chen Allen G. Ross Donald P. McManus |
| author_sort | Pengfei Cai |
| collection | DOAJ |
| description | Schistosoma japonicum is stubbornly persistent in China and the Philippines. Fast and accurate diagnostic tools are required to monitor effective control measures against schistosomiasis japonica. Promising antigen candidates for the serological diagnosis of schistosomiasis japonica have generally been identified from the Chinese strain of S. japonicum. However, the Chinese (SjC) and Philippine (SjP) strains of S. japonicum express a number of clear phenotypic differences, including aspects of host immune responses. This feature thereby emphasized the requirement to determine whether antigens identified as having diagnostic value for SjC infection are also suitable for the diagnosis of SjP infection. In the current study, 10 antigens were selected for comparison of diagnostic performance of the SjP infection using ELISA. On testing of sera from 180 subjects in the Philippines, SjSAP4 exhibited the best diagnostic performance with 94.03% sensitivity and 98.33% specificity using an optimized serum dilution. In another large scale testing with 412 serum samples, a combination (SjSAP4 + Sj23-LHD (large hydrophilic domain)) provided the best diagnostic outcome with 87.04% sensitivity and 96.67% specificity. This combination could be used in future for serological diagnosis of schistosomiasis in the Philippines, thereby representing an important component for monitoring integrated control measures. |
| first_indexed | 2024-12-11T08:59:16Z |
| format | Article |
| id | doaj.art-f88d39fd94e445cab52fb737f65501c7 |
| institution | Directory Open Access Journal |
| issn | 2352-3964 |
| language | English |
| last_indexed | 2024-12-11T08:59:16Z |
| publishDate | 2017-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | EBioMedicine |
| spelling | doaj.art-f88d39fd94e445cab52fb737f65501c72022-12-22T01:13:48ZengElsevierEBioMedicine2352-39642017-10-0124C23724610.1016/j.ebiom.2017.09.011A Parallel Comparison of Antigen Candidates for Development of an Optimized Serological Diagnosis of Schistosomiasis Japonica in the PhilippinesPengfei Cai0Kosala G. Weerakoon1Yi Mu2David U. Olveda3Xianyu Piao4Shuai Liu5Remigio M. Olveda6Qijun Chen7Allen G. Ross8Donald P. McManus9Molecular Parasitology Laboratory, QIMR Berghofer Medical Research Institute, Queensland, AustraliaMolecular Parasitology Laboratory, QIMR Berghofer Medical Research Institute, Queensland, AustraliaMolecular Parasitology Laboratory, QIMR Berghofer Medical Research Institute, Queensland, AustraliaMenzies Health Institute Queensland, Griffith University, Gold Coast, AustraliaMOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, PR ChinaMOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, PR ChinaResearch Institute for Tropical Medicine, Department of Health, Manila, PhilippinesKey Laboratory of Zoonosis, Shangyang Agricultural University, Shengyang, PR ChinaMenzies Health Institute Queensland, Griffith University, Gold Coast, AustraliaMolecular Parasitology Laboratory, QIMR Berghofer Medical Research Institute, Queensland, AustraliaSchistosoma japonicum is stubbornly persistent in China and the Philippines. Fast and accurate diagnostic tools are required to monitor effective control measures against schistosomiasis japonica. Promising antigen candidates for the serological diagnosis of schistosomiasis japonica have generally been identified from the Chinese strain of S. japonicum. However, the Chinese (SjC) and Philippine (SjP) strains of S. japonicum express a number of clear phenotypic differences, including aspects of host immune responses. This feature thereby emphasized the requirement to determine whether antigens identified as having diagnostic value for SjC infection are also suitable for the diagnosis of SjP infection. In the current study, 10 antigens were selected for comparison of diagnostic performance of the SjP infection using ELISA. On testing of sera from 180 subjects in the Philippines, SjSAP4 exhibited the best diagnostic performance with 94.03% sensitivity and 98.33% specificity using an optimized serum dilution. In another large scale testing with 412 serum samples, a combination (SjSAP4 + Sj23-LHD (large hydrophilic domain)) provided the best diagnostic outcome with 87.04% sensitivity and 96.67% specificity. This combination could be used in future for serological diagnosis of schistosomiasis in the Philippines, thereby representing an important component for monitoring integrated control measures.http://www.sciencedirect.com/science/article/pii/S235239641730364XSchistosomiasisSchistosoma japonicumPhilippine strainSerological diagnosisSjSAP4Sj23-LHD |
| spellingShingle | Pengfei Cai Kosala G. Weerakoon Yi Mu David U. Olveda Xianyu Piao Shuai Liu Remigio M. Olveda Qijun Chen Allen G. Ross Donald P. McManus A Parallel Comparison of Antigen Candidates for Development of an Optimized Serological Diagnosis of Schistosomiasis Japonica in the Philippines EBioMedicine Schistosomiasis Schistosoma japonicum Philippine strain Serological diagnosis SjSAP4 Sj23-LHD |
| title | A Parallel Comparison of Antigen Candidates for Development of an Optimized Serological Diagnosis of Schistosomiasis Japonica in the Philippines |
| title_full | A Parallel Comparison of Antigen Candidates for Development of an Optimized Serological Diagnosis of Schistosomiasis Japonica in the Philippines |
| title_fullStr | A Parallel Comparison of Antigen Candidates for Development of an Optimized Serological Diagnosis of Schistosomiasis Japonica in the Philippines |
| title_full_unstemmed | A Parallel Comparison of Antigen Candidates for Development of an Optimized Serological Diagnosis of Schistosomiasis Japonica in the Philippines |
| title_short | A Parallel Comparison of Antigen Candidates for Development of an Optimized Serological Diagnosis of Schistosomiasis Japonica in the Philippines |
| title_sort | parallel comparison of antigen candidates for development of an optimized serological diagnosis of schistosomiasis japonica in the philippines |
| topic | Schistosomiasis Schistosoma japonicum Philippine strain Serological diagnosis SjSAP4 Sj23-LHD |
| url | http://www.sciencedirect.com/science/article/pii/S235239641730364X |
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