Hemin with Peroxidase Activity Can Inhibit the Oxidative Damage Induced by Ultraviolet A

Excessive reactive oxygen species (ROS), a highly reactive substance that contains oxygen, induced by ultraviolet A (UVA) cause oxidative damage to skin. We confirmed that hemin can catalyze the reaction of tyrosine (Tyr) and hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>). Ca...

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Bibliographic Details
Main Authors: Wenli Hui, Zhipeng Yang, Ke Fang, Mengdi Wu, Wenhua Mu, Cong Zhao, Dan Xue, Tengteng Zhu, Xiao Li, Ming Gao, Yunhua Lu, Kunping Yan
Format: Article
Language:English
Published: MDPI AG 2022-06-01
Series:Current Issues in Molecular Biology
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Online Access:https://www.mdpi.com/1467-3045/44/6/183
Description
Summary:Excessive reactive oxygen species (ROS), a highly reactive substance that contains oxygen, induced by ultraviolet A (UVA) cause oxidative damage to skin. We confirmed that hemin can catalyze the reaction of tyrosine (Tyr) and hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>). Catalysis was found to effectively reduce or eliminate oxidative damage to cells induced by H<sub>2</sub>O<sub>2</sub> or UVA. The scavenging effects of hemin for other free-radical ROS were also evaluated through pyrogallol autoxidation, 1,1-diphenyl-2-picrylhydrazyl radical (DPPH·)-scavenging assays, and phenanthroline–Fe<sup>2+</sup> assays. The results show that a mixture of hemin and tyrosine exhibits strong scavenging activities for H<sub>2</sub>O<sub>2</sub>, superoxide anion (O<sub>2</sub><sup>−</sup>·), DPPH·, and the hydroxyl radical (·OH). Furthermore, the inhibition of oxidative damage to human skin keratinocyte (HaCaT) cells induced by H<sub>2</sub>O<sub>2</sub> or UVA was evaluated. The results show that catalysis can significantly reduce the ratio of cell apoptosis and death and inhibit the release of lactate dehydrogenase (LDH), as well as accumulation of malondialdehyde (MDA). Furthermore, the resistance to apoptosis was found to be enhanced. These results show that the mixture of hemin and tyrosine has a significantly protective effect against oxidative damage to HaCaT cells caused by UVA, suggesting it as a protective agent for combating UVA damage.
ISSN:1467-3037
1467-3045