Immunohistochemical and immunocytochemical study of mechanoreceptors in anterior cruciate ligament reconstruction with the remnant-preserving technique using Achilles tendon allografts
Abstract Background Attempts have been made to validate the significance of remnant preservation with anterior cruciate ligament (ACL) reconstruction using immunohistochemical and immunocytochemical techniques. The purpose of this study was to examine the expression of mechanoreceptors in the remnan...
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| Format: | Article |
| Language: | English |
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BMC
2017-06-01
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| Series: | Journal of Orthopaedic Surgery and Research |
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| Online Access: | http://link.springer.com/article/10.1186/s13018-017-0593-0 |
| _version_ | 1798038016315359232 |
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| author | Keun Churl Chun Sung Hyun Lee Jeong Woo Kim Eun Jung Jin Kwang Mee Kim Churl Hong Chun |
| author_facet | Keun Churl Chun Sung Hyun Lee Jeong Woo Kim Eun Jung Jin Kwang Mee Kim Churl Hong Chun |
| author_sort | Keun Churl Chun |
| collection | DOAJ |
| description | Abstract Background Attempts have been made to validate the significance of remnant preservation with anterior cruciate ligament (ACL) reconstruction using immunohistochemical and immunocytochemical techniques. The purpose of this study was to examine the expression of mechanoreceptors in the remnant tissue of ACL reconstruction performed with the remnant-preserving technique. Methods Tissue samples were obtained from 10 patients who underwent ACL reconstruction with the remnant-preserving technique. The specimens were obtained from remnant ACL tissue and Achilles allografts superficially and at the tibial attachment. The control group consisted of three normal ACLs procured from young males who underwent partial meniscectomy. Tissues and cells from the ACL remnants and Achilles allografts were characterized using hematoxylin and eosin (H&E) staining and immunohistochemical, immunocytochemical, and immunoblotting assays. In particular, the sensitivity of neural cell validation was improved using nerve growth factor (NGF) to stimulate the expression of neural cells. Results The results are summarized as follows. (1) In H&E staining and immunohistochemical assays, no neural cells were detected in remnant or allograft tissue. (2) In the immunocytochemical study, neural cells were detected in remnant tissue. (3) The increased proliferation of remnant ACL cells with NGF treatment suggested their identity as neural cells. (4) NGF treatment also stimulated protein and RNA expression of Nestin (a specific marker for neural cells) in remnant ACL cells. Conclusions The improved immunocytochemical methodology proved useful. Although mechanoreceptors were detected relatively less frequently than expected, the authors consider that this finding does not negate the necessity of remnant-preserving ACL reconstruction. |
| first_indexed | 2024-04-11T21:34:27Z |
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| id | doaj.art-f88f1c62d8fb403fbdf3cec148d82f44 |
| institution | Directory Open Access Journal |
| issn | 1749-799X |
| language | English |
| last_indexed | 2024-04-11T21:34:27Z |
| publishDate | 2017-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Orthopaedic Surgery and Research |
| spelling | doaj.art-f88f1c62d8fb403fbdf3cec148d82f442022-12-22T04:01:48ZengBMCJournal of Orthopaedic Surgery and Research1749-799X2017-06-011211810.1186/s13018-017-0593-0Immunohistochemical and immunocytochemical study of mechanoreceptors in anterior cruciate ligament reconstruction with the remnant-preserving technique using Achilles tendon allograftsKeun Churl Chun0Sung Hyun Lee1Jeong Woo Kim2Eun Jung Jin3Kwang Mee Kim4Churl Hong Chun5Naval Hospital of Korean Armed ForcesDepartment of Orthopedic Surgery, School of Medicine, Wonkwang University HospitalDepartment of Orthopedic Surgery, School of Medicine, Wonkwang University HospitalDepartment of Biological Sciences, College of Natural Sciences, Wonkwang UniversityDepartment of Nursing, Chodang UniversityDepartment of Orthopedic Surgery, School of Medicine, Wonkwang University HospitalAbstract Background Attempts have been made to validate the significance of remnant preservation with anterior cruciate ligament (ACL) reconstruction using immunohistochemical and immunocytochemical techniques. The purpose of this study was to examine the expression of mechanoreceptors in the remnant tissue of ACL reconstruction performed with the remnant-preserving technique. Methods Tissue samples were obtained from 10 patients who underwent ACL reconstruction with the remnant-preserving technique. The specimens were obtained from remnant ACL tissue and Achilles allografts superficially and at the tibial attachment. The control group consisted of three normal ACLs procured from young males who underwent partial meniscectomy. Tissues and cells from the ACL remnants and Achilles allografts were characterized using hematoxylin and eosin (H&E) staining and immunohistochemical, immunocytochemical, and immunoblotting assays. In particular, the sensitivity of neural cell validation was improved using nerve growth factor (NGF) to stimulate the expression of neural cells. Results The results are summarized as follows. (1) In H&E staining and immunohistochemical assays, no neural cells were detected in remnant or allograft tissue. (2) In the immunocytochemical study, neural cells were detected in remnant tissue. (3) The increased proliferation of remnant ACL cells with NGF treatment suggested their identity as neural cells. (4) NGF treatment also stimulated protein and RNA expression of Nestin (a specific marker for neural cells) in remnant ACL cells. Conclusions The improved immunocytochemical methodology proved useful. Although mechanoreceptors were detected relatively less frequently than expected, the authors consider that this finding does not negate the necessity of remnant-preserving ACL reconstruction.http://link.springer.com/article/10.1186/s13018-017-0593-0Anterior cruciate ligamentRemnant-preserving techniqueImmunohistochemical study of mechanoreceptorImmunocytochemical study of mechanoreceptor |
| spellingShingle | Keun Churl Chun Sung Hyun Lee Jeong Woo Kim Eun Jung Jin Kwang Mee Kim Churl Hong Chun Immunohistochemical and immunocytochemical study of mechanoreceptors in anterior cruciate ligament reconstruction with the remnant-preserving technique using Achilles tendon allografts Journal of Orthopaedic Surgery and Research Anterior cruciate ligament Remnant-preserving technique Immunohistochemical study of mechanoreceptor Immunocytochemical study of mechanoreceptor |
| title | Immunohistochemical and immunocytochemical study of mechanoreceptors in anterior cruciate ligament reconstruction with the remnant-preserving technique using Achilles tendon allografts |
| title_full | Immunohistochemical and immunocytochemical study of mechanoreceptors in anterior cruciate ligament reconstruction with the remnant-preserving technique using Achilles tendon allografts |
| title_fullStr | Immunohistochemical and immunocytochemical study of mechanoreceptors in anterior cruciate ligament reconstruction with the remnant-preserving technique using Achilles tendon allografts |
| title_full_unstemmed | Immunohistochemical and immunocytochemical study of mechanoreceptors in anterior cruciate ligament reconstruction with the remnant-preserving technique using Achilles tendon allografts |
| title_short | Immunohistochemical and immunocytochemical study of mechanoreceptors in anterior cruciate ligament reconstruction with the remnant-preserving technique using Achilles tendon allografts |
| title_sort | immunohistochemical and immunocytochemical study of mechanoreceptors in anterior cruciate ligament reconstruction with the remnant preserving technique using achilles tendon allografts |
| topic | Anterior cruciate ligament Remnant-preserving technique Immunohistochemical study of mechanoreceptor Immunocytochemical study of mechanoreceptor |
| url | http://link.springer.com/article/10.1186/s13018-017-0593-0 |
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