Assesment of artificial caries lesions through scanning electron microscopy and cross-sectional microhardness test
Aims: To assess through scanning electron microscopy (SEM) and cross-sectional microhardness (CSMH) test whether the methodology exposed in this experiment can be used to produce artificial active white spot lesions (AAWSLs) on smooth unabraded human dental enamel. Materials and Methods: Ten human p...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2013-01-01
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| Series: | Indian Journal of Dental Research |
| Subjects: | |
| Online Access: | http://www.ijdr.in/article.asp?issn=0970-9290;year=2013;volume=24;issue=2;spage=249;epage=254;aulast=de |
| _version_ | 1818971250189402112 |
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| author | Mirian de Waele Souchois de Marsillac Ricardo de Sousa Vieira |
| author_facet | Mirian de Waele Souchois de Marsillac Ricardo de Sousa Vieira |
| author_sort | Mirian de Waele Souchois de Marsillac |
| collection | DOAJ |
| description | Aims: To assess through scanning electron microscopy (SEM) and cross-sectional microhardness (CSMH) test whether the methodology exposed in this experiment can be used to produce artificial active white spot lesions (AAWSLs) on smooth unabraded human dental enamel.
Materials and Methods: Ten human permanent molars were used in this experiment. One section of each tooth was double coated with nail varnish except for a limited central area sized 2.5 mm × 1 mm (2.5 mm 2 ). Each specimen was individually exposed to 10.4 ml of a demineralizing solution at pH 5.0, during 42 days (37°C) without agitation. Samples were sectioned in the center of the AAWSL and one half was analyzed in SEM and the other half was subjected to CSMH. Descriptive statistics was performed to determine mean depth of the lesion.
Results: The mean depth of AAWSL was 100 μm (s.d. =12.1) and a white dull rough surface could be detected by the unaided eye. SEM images demonstrated that although some surface areas of the lesion appeared to be relatively intact, erosion was present. A prismatic pattern of dissolution was observed in all samples with an enlargement of the prism sheaths and some samples had also sites of destruction of prism cores.
Conclusion: This methodology can be used to induce AAWSLs in human dental enamel but surface erosion has to be taken into account when performing CSMH test. |
| first_indexed | 2024-12-20T14:49:23Z |
| format | Article |
| id | doaj.art-f89039d864374224ac6e5971e7c1032a |
| institution | Directory Open Access Journal |
| issn | 0970-9290 1998-3603 |
| language | English |
| last_indexed | 2024-12-20T14:49:23Z |
| publishDate | 2013-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Indian Journal of Dental Research |
| spelling | doaj.art-f89039d864374224ac6e5971e7c1032a2022-12-21T19:37:01ZengWolters Kluwer Medknow PublicationsIndian Journal of Dental Research0970-92901998-36032013-01-0124224925410.4103/0970-9290.116699Assesment of artificial caries lesions through scanning electron microscopy and cross-sectional microhardness testMirian de Waele Souchois de MarsillacRicardo de Sousa VieiraAims: To assess through scanning electron microscopy (SEM) and cross-sectional microhardness (CSMH) test whether the methodology exposed in this experiment can be used to produce artificial active white spot lesions (AAWSLs) on smooth unabraded human dental enamel. Materials and Methods: Ten human permanent molars were used in this experiment. One section of each tooth was double coated with nail varnish except for a limited central area sized 2.5 mm × 1 mm (2.5 mm 2 ). Each specimen was individually exposed to 10.4 ml of a demineralizing solution at pH 5.0, during 42 days (37°C) without agitation. Samples were sectioned in the center of the AAWSL and one half was analyzed in SEM and the other half was subjected to CSMH. Descriptive statistics was performed to determine mean depth of the lesion. Results: The mean depth of AAWSL was 100 μm (s.d. =12.1) and a white dull rough surface could be detected by the unaided eye. SEM images demonstrated that although some surface areas of the lesion appeared to be relatively intact, erosion was present. A prismatic pattern of dissolution was observed in all samples with an enlargement of the prism sheaths and some samples had also sites of destruction of prism cores. Conclusion: This methodology can be used to induce AAWSLs in human dental enamel but surface erosion has to be taken into account when performing CSMH test.http://www.ijdr.in/article.asp?issn=0970-9290;year=2013;volume=24;issue=2;spage=249;epage=254;aulast=deDental cariesdental enamelelectron scanningmicroscopyultrastructure |
| spellingShingle | Mirian de Waele Souchois de Marsillac Ricardo de Sousa Vieira Assesment of artificial caries lesions through scanning electron microscopy and cross-sectional microhardness test Indian Journal of Dental Research Dental caries dental enamel electron scanning microscopy ultrastructure |
| title | Assesment of artificial caries lesions through scanning electron microscopy and cross-sectional microhardness test |
| title_full | Assesment of artificial caries lesions through scanning electron microscopy and cross-sectional microhardness test |
| title_fullStr | Assesment of artificial caries lesions through scanning electron microscopy and cross-sectional microhardness test |
| title_full_unstemmed | Assesment of artificial caries lesions through scanning electron microscopy and cross-sectional microhardness test |
| title_short | Assesment of artificial caries lesions through scanning electron microscopy and cross-sectional microhardness test |
| title_sort | assesment of artificial caries lesions through scanning electron microscopy and cross sectional microhardness test |
| topic | Dental caries dental enamel electron scanning microscopy ultrastructure |
| url | http://www.ijdr.in/article.asp?issn=0970-9290;year=2013;volume=24;issue=2;spage=249;epage=254;aulast=de |
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