Proteome of <i>Staphylococcus aureus</i> Biofilm Changes Significantly with Aging
<i>Staphylococcus aureus</i> is a notorious biofilm-producing pathogen that is frequently isolated from implantable medical device infections. As biofilm ages, it becomes more tolerant to antimicrobial treatment leading to treatment failure and necessitating the costly removal of infecte...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-06-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/12/6415 |
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| author | Md. Arifur Rahman Ardeshir Amirkhani Durdana Chowdhury Maria Mempin Mark P. Molloy Anand Kumar Deva Karen Vickery Honghua Hu |
| author_facet | Md. Arifur Rahman Ardeshir Amirkhani Durdana Chowdhury Maria Mempin Mark P. Molloy Anand Kumar Deva Karen Vickery Honghua Hu |
| author_sort | Md. Arifur Rahman |
| collection | DOAJ |
| description | <i>Staphylococcus aureus</i> is a notorious biofilm-producing pathogen that is frequently isolated from implantable medical device infections. As biofilm ages, it becomes more tolerant to antimicrobial treatment leading to treatment failure and necessitating the costly removal of infected devices. In this study, we performed in-solution digestion followed by TMT-based high-throughput mass spectrometry and investigated what changes occur in the proteome of <i>S. aureus</i> biofilm grown for 3-days and 12-days in comparison with 24 h planktonic. It showed that proteins associated with biosynthetic processes, ABC transporter pathway, virulence proteins, and shikimate kinase pathway were significantly upregulated in a 3-day biofilm, while proteins associated with sugar transporter, degradation, and stress response were downregulated. Interestingly, in a 3-day biofilm, we observed numerous proteins involved in the central metabolism pathways which could lead to biofilm growth under diverse environments by providing an alternative metabolic route to utilize energy. In 12-day biofilms, proteins associated with peptidoglycan biosynthesis, sugar transporters, and stress responses were upregulated, whereas proteins associated with ABC transporters, DNA replication, and adhesion proteins were downregulated. Gene Ontology analysis revealed that more proteins are involved in metabolic processes in 3dwb compared with 12dwb. Furthermore, we observed significant variations in the formation of biofilms resulting from changes in the level of metabolic activity in the different growth modes of biofilms that could be a significant factor in <i>S. aureus</i> biofilm maturation and persistence. Collectively, potential marker proteins were identified and further characterized to understand their exact role in <i>S. aureus</i> biofilm development, which may shed light on possible new therapeutic regimes in the treatment of biofilm-related implant-associated infections. |
| first_indexed | 2024-03-09T23:36:36Z |
| format | Article |
| id | doaj.art-f890bbc4787c48fe83e20e05b67ab1f1 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T23:36:36Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-f890bbc4787c48fe83e20e05b67ab1f12023-11-23T17:00:24ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-06-012312641510.3390/ijms23126415Proteome of <i>Staphylococcus aureus</i> Biofilm Changes Significantly with AgingMd. Arifur Rahman0Ardeshir Amirkhani1Durdana Chowdhury2Maria Mempin3Mark P. Molloy4Anand Kumar Deva5Karen Vickery6Honghua Hu7Surgical Infection Research Group, Faculty of Medicine, Health and Human Sciences, Macquarie University, Sydney 2109, AustraliaAustralian Proteome Analysis Facility, Macquarie University, Sydney 2109, AustraliaSurgical Infection Research Group, Faculty of Medicine, Health and Human Sciences, Macquarie University, Sydney 2109, AustraliaSurgical Infection Research Group, Faculty of Medicine, Health and Human Sciences, Macquarie University, Sydney 2109, AustraliaAustralian Proteome Analysis Facility, Macquarie University, Sydney 2109, AustraliaSurgical Infection Research Group, Faculty of Medicine, Health and Human Sciences, Macquarie University, Sydney 2109, AustraliaSurgical Infection Research Group, Faculty of Medicine, Health and Human Sciences, Macquarie University, Sydney 2109, AustraliaSurgical Infection Research Group, Faculty of Medicine, Health and Human Sciences, Macquarie University, Sydney 2109, Australia<i>Staphylococcus aureus</i> is a notorious biofilm-producing pathogen that is frequently isolated from implantable medical device infections. As biofilm ages, it becomes more tolerant to antimicrobial treatment leading to treatment failure and necessitating the costly removal of infected devices. In this study, we performed in-solution digestion followed by TMT-based high-throughput mass spectrometry and investigated what changes occur in the proteome of <i>S. aureus</i> biofilm grown for 3-days and 12-days in comparison with 24 h planktonic. It showed that proteins associated with biosynthetic processes, ABC transporter pathway, virulence proteins, and shikimate kinase pathway were significantly upregulated in a 3-day biofilm, while proteins associated with sugar transporter, degradation, and stress response were downregulated. Interestingly, in a 3-day biofilm, we observed numerous proteins involved in the central metabolism pathways which could lead to biofilm growth under diverse environments by providing an alternative metabolic route to utilize energy. In 12-day biofilms, proteins associated with peptidoglycan biosynthesis, sugar transporters, and stress responses were upregulated, whereas proteins associated with ABC transporters, DNA replication, and adhesion proteins were downregulated. Gene Ontology analysis revealed that more proteins are involved in metabolic processes in 3dwb compared with 12dwb. Furthermore, we observed significant variations in the formation of biofilms resulting from changes in the level of metabolic activity in the different growth modes of biofilms that could be a significant factor in <i>S. aureus</i> biofilm maturation and persistence. Collectively, potential marker proteins were identified and further characterized to understand their exact role in <i>S. aureus</i> biofilm development, which may shed light on possible new therapeutic regimes in the treatment of biofilm-related implant-associated infections.https://www.mdpi.com/1422-0067/23/12/6415<i>Staphylococcus aureus</i>biofilmsproteomeTMTmass spectrometryaging |
| spellingShingle | Md. Arifur Rahman Ardeshir Amirkhani Durdana Chowdhury Maria Mempin Mark P. Molloy Anand Kumar Deva Karen Vickery Honghua Hu Proteome of <i>Staphylococcus aureus</i> Biofilm Changes Significantly with Aging International Journal of Molecular Sciences <i>Staphylococcus aureus</i> biofilms proteome TMT mass spectrometry aging |
| title | Proteome of <i>Staphylococcus aureus</i> Biofilm Changes Significantly with Aging |
| title_full | Proteome of <i>Staphylococcus aureus</i> Biofilm Changes Significantly with Aging |
| title_fullStr | Proteome of <i>Staphylococcus aureus</i> Biofilm Changes Significantly with Aging |
| title_full_unstemmed | Proteome of <i>Staphylococcus aureus</i> Biofilm Changes Significantly with Aging |
| title_short | Proteome of <i>Staphylococcus aureus</i> Biofilm Changes Significantly with Aging |
| title_sort | proteome of i staphylococcus aureus i biofilm changes significantly with aging |
| topic | <i>Staphylococcus aureus</i> biofilms proteome TMT mass spectrometry aging |
| url | https://www.mdpi.com/1422-0067/23/12/6415 |
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