Differences in IL-1β and IL-6 levels in the gingival crevicular fluid during acute phase of orthodontic tooth movement between juveniles and young adults
Background/Aim. There is little information, about the difference in cytokine levels in the gingival crevicular fluid (GCF) during orthodontic tooth movement (OTM), between juveniles (children) and young adults (adults). The aim of this study was to examine the levels of interleukins IL-1β and IL-...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Military Health Department, Ministry of Defence, Serbia
2017-01-01
|
| Series: | Vojnosanitetski Pregled |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0042-8450/2017/0042-84501600178V.pdf |
| _version_ | 1826574748664987648 |
|---|---|
| author | Vujačić Amila Konić Aleksandra Pavlović Jasna Todorović Vera Vukićević Vladanka Jevremović Danimir Milošević-Jovčić Nadežda |
| author_facet | Vujačić Amila Konić Aleksandra Pavlović Jasna Todorović Vera Vukićević Vladanka Jevremović Danimir Milošević-Jovčić Nadežda |
| author_sort | Vujačić Amila |
| collection | DOAJ |
| description | Background/Aim. There is little information, about the difference in cytokine levels in the gingival crevicular fluid (GCF) during orthodontic tooth movement (OTM), between juveniles (children) and young adults (adults). The aim of this study was to examine the levels of interleukins IL-1β and IL- 6 in GCF of these two age groups during the acute phase of OTM. Methods. The subjects, 10 children and 10 adults, underwent OTM of a single tooth, with an untreated antagonistic tooth used as the control group. GCF was sampled from both the control and treatment sites right before the beginning (the baseline) and 24 h, 72 h and 168 h upon initiation of OTM. Cytokine levels were determined by enzymelinked immunosorbent assay (ELISA). Results. The levels of both GCF IL-1β and IL-6 showed a bimodal peak during early phase of OTM, at 24 h and 168 h, in both age groups. As the statistic has shown, the increase in IL-1β levels was more prominent after 168 h in treated teeth of children, compared to both children’s control teeth and treated teeth of adults, whilst the GCF IL-6 levels in the same group increased significantly after 24 h, as well as after 168 h, approximately 70 and 55 fold, respectively. In the same time periods the increase of IL-6 levels in GCF of adults was notably lesser, averaging approximately 5 and 10 fold, respectively, compared to the control teeth. In addition, the amount of tooth movement was statistically larger for children than for adults 168 hours upon the initiation of OTM. Conclusion. GCF IL-1β and IL-6 were increasingly expressed during initial phase of OTM in both children and adults. However, excretory response of cytokines in children’s GCF, especially the concentration of IL-6, was at a significantly higher level than that of adults’, which accords to the finding that the initial OTM is faster in children. |
| first_indexed | 2024-12-12T03:05:35Z |
| format | Article |
| id | doaj.art-f8912dce5eb049769ae3ce0bf36748e8 |
| institution | Directory Open Access Journal |
| issn | 0042-8450 2406-0720 |
| language | English |
| last_indexed | 2025-03-14T13:03:44Z |
| publishDate | 2017-01-01 |
| publisher | Military Health Department, Ministry of Defence, Serbia |
| record_format | Article |
| series | Vojnosanitetski Pregled |
| spelling | doaj.art-f8912dce5eb049769ae3ce0bf36748e82025-03-02T02:38:53ZengMilitary Health Department, Ministry of Defence, SerbiaVojnosanitetski Pregled0042-84502406-07202017-01-0174321922610.2298/VSP150425178V0042-84501600178VDifferences in IL-1β and IL-6 levels in the gingival crevicular fluid during acute phase of orthodontic tooth movement between juveniles and young adultsVujačić Amila0Konić Aleksandra1Pavlović Jasna2Todorović Vera3Vukićević Vladanka4Jevremović Danimir5Milošević-Jovčić Nadežda6Faculty of Medicine, Department of Dentistry, Kosovska MitrovicaInstitute for Medical Research, BelgradeFaculty of Medicine, Department of Dentistry, Kosovska MitrovicaUniversity of Busines Academy of Novi Sad, Faculty of Stomatology, PančevoFaculty of Medicine, Department of Dentistry, Kosovska MitrovicaUniversity of Busines Academy of Novi Sad, Faculty of Stomatology, PančevoInstitute for Medical Research, BelgradeBackground/Aim. There is little information, about the difference in cytokine levels in the gingival crevicular fluid (GCF) during orthodontic tooth movement (OTM), between juveniles (children) and young adults (adults). The aim of this study was to examine the levels of interleukins IL-1β and IL- 6 in GCF of these two age groups during the acute phase of OTM. Methods. The subjects, 10 children and 10 adults, underwent OTM of a single tooth, with an untreated antagonistic tooth used as the control group. GCF was sampled from both the control and treatment sites right before the beginning (the baseline) and 24 h, 72 h and 168 h upon initiation of OTM. Cytokine levels were determined by enzymelinked immunosorbent assay (ELISA). Results. The levels of both GCF IL-1β and IL-6 showed a bimodal peak during early phase of OTM, at 24 h and 168 h, in both age groups. As the statistic has shown, the increase in IL-1β levels was more prominent after 168 h in treated teeth of children, compared to both children’s control teeth and treated teeth of adults, whilst the GCF IL-6 levels in the same group increased significantly after 24 h, as well as after 168 h, approximately 70 and 55 fold, respectively. In the same time periods the increase of IL-6 levels in GCF of adults was notably lesser, averaging approximately 5 and 10 fold, respectively, compared to the control teeth. In addition, the amount of tooth movement was statistically larger for children than for adults 168 hours upon the initiation of OTM. Conclusion. GCF IL-1β and IL-6 were increasingly expressed during initial phase of OTM in both children and adults. However, excretory response of cytokines in children’s GCF, especially the concentration of IL-6, was at a significantly higher level than that of adults’, which accords to the finding that the initial OTM is faster in children.http://www.doiserbia.nb.rs/img/doi/0042-8450/2017/0042-84501600178V.pdftooth movementchildadolescentadultinterleukin- 6interleukin-1 betaacute-phase proteins |
| spellingShingle | Vujačić Amila Konić Aleksandra Pavlović Jasna Todorović Vera Vukićević Vladanka Jevremović Danimir Milošević-Jovčić Nadežda Differences in IL-1β and IL-6 levels in the gingival crevicular fluid during acute phase of orthodontic tooth movement between juveniles and young adults Vojnosanitetski Pregled tooth movement child adolescent adult interleukin- 6 interleukin-1 beta acute-phase proteins |
| title | Differences in IL-1β and IL-6 levels in the gingival crevicular fluid during acute phase of orthodontic tooth movement between juveniles and young adults |
| title_full | Differences in IL-1β and IL-6 levels in the gingival crevicular fluid during acute phase of orthodontic tooth movement between juveniles and young adults |
| title_fullStr | Differences in IL-1β and IL-6 levels in the gingival crevicular fluid during acute phase of orthodontic tooth movement between juveniles and young adults |
| title_full_unstemmed | Differences in IL-1β and IL-6 levels in the gingival crevicular fluid during acute phase of orthodontic tooth movement between juveniles and young adults |
| title_short | Differences in IL-1β and IL-6 levels in the gingival crevicular fluid during acute phase of orthodontic tooth movement between juveniles and young adults |
| title_sort | differences in il 1β and il 6 levels in the gingival crevicular fluid during acute phase of orthodontic tooth movement between juveniles and young adults |
| topic | tooth movement child adolescent adult interleukin- 6 interleukin-1 beta acute-phase proteins |
| url | http://www.doiserbia.nb.rs/img/doi/0042-8450/2017/0042-84501600178V.pdf |
| work_keys_str_mv | AT vujacicamila differencesinil1bandil6levelsinthegingivalcrevicularfluidduringacutephaseoforthodontictoothmovementbetweenjuvenilesandyoungadults AT konicaleksandra differencesinil1bandil6levelsinthegingivalcrevicularfluidduringacutephaseoforthodontictoothmovementbetweenjuvenilesandyoungadults AT pavlovicjasna differencesinil1bandil6levelsinthegingivalcrevicularfluidduringacutephaseoforthodontictoothmovementbetweenjuvenilesandyoungadults AT todorovicvera differencesinil1bandil6levelsinthegingivalcrevicularfluidduringacutephaseoforthodontictoothmovementbetweenjuvenilesandyoungadults AT vukicevicvladanka differencesinil1bandil6levelsinthegingivalcrevicularfluidduringacutephaseoforthodontictoothmovementbetweenjuvenilesandyoungadults AT jevremovicdanimir differencesinil1bandil6levelsinthegingivalcrevicularfluidduringacutephaseoforthodontictoothmovementbetweenjuvenilesandyoungadults AT milosevicjovcicnadezda differencesinil1bandil6levelsinthegingivalcrevicularfluidduringacutephaseoforthodontictoothmovementbetweenjuvenilesandyoungadults |