Osteoblasts subjected to tensile force induce osteoclastic differentiation of murine macrophages in a coculture system
Background/purpose: Bone remodeling is regulated by the replacement of old bone with new bone through sequential osteoclastic resorption and osteoblastic bone formation. In this study, we compare the functional difference in osteoclastogenesis of murine macrophage when applied to the receptor activa...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2015-03-01
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| Series: | Journal of Dental Sciences |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1991790213001736 |
| _version_ | 1818962605155287040 |
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| author | Yi-Jyun Chen Ming-You Shie Chi-Jr Hung Shiau-Lee Liu Tsui-Hsien Huang Chia-Tze Kao |
| author_facet | Yi-Jyun Chen Ming-You Shie Chi-Jr Hung Shiau-Lee Liu Tsui-Hsien Huang Chia-Tze Kao |
| author_sort | Yi-Jyun Chen |
| collection | DOAJ |
| description | Background/purpose: Bone remodeling is regulated by the replacement of old bone with new bone through sequential osteoclastic resorption and osteoblastic bone formation. In this study, we compare the functional difference in osteoclastogenesis of murine macrophage when applied to the receptor activator of nuclear factor kappa B ligand (RANKL) and osteoprotegerin (OPG) produced by osteoblasts in response to tensile stress in a coculture system.
Materials and methods: We examined the secretion of RANKL and OPG at different time points when the osteoblast cells are being cultured under tensile force. In order to confirm whether the media produced by the osteoblast cells under tensile force induce osteoclastogenesis or not, murine macrophage (RAW264.7 cells) were cocultured with osteoblasts.
Results: In both mono- and coculture systems, the two cell types cultured under tensile force and in normal environment showed no significant differences (P > 0.05) at any time. However, in the coculture system, the production of tartrate-resistant acid phosphatase (TRAP) in RAW264.7 cells under tensile force was found to increase significantly (P < 0.05; by 2.27-, 3.00-, or 3.27-fold on Day 3, Day 7, or Day 15, respectively) when compared with the corresponding TRAP activity in RAW264.7 cells in normal environment. Moreover, the results indicate that the tensile force upregulated the secretion of RANKL and inhibited OPG synthesis. Therefore, RAW264.7 cells appear to increase their production of TRAP in the media of osteoblasts under tensile force, increasing TRAP activity by nearly 2.8 times compared to that in the media of osteoblasts in a normal environment for 3 days.
Conclusion: These results suggest that osteoblasts influence the secretion of RANKL more than OPG when stimulated with osteoclastogenesis via RANKL under tensile force. |
| first_indexed | 2024-12-20T12:31:59Z |
| format | Article |
| id | doaj.art-f892abd45b264e00819ff660d3c8539e |
| institution | Directory Open Access Journal |
| issn | 1991-7902 |
| language | English |
| last_indexed | 2024-12-20T12:31:59Z |
| publishDate | 2015-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Dental Sciences |
| spelling | doaj.art-f892abd45b264e00819ff660d3c8539e2022-12-21T19:40:42ZengElsevierJournal of Dental Sciences1991-79022015-03-01101818710.1016/j.jds.2013.11.003Osteoblasts subjected to tensile force induce osteoclastic differentiation of murine macrophages in a coculture systemYi-Jyun Chen0Ming-You Shie1Chi-Jr Hung2Shiau-Lee Liu3Tsui-Hsien Huang4Chia-Tze Kao5School of Dentistry, Chung Shan Medical University, Taichung City, TaiwanInstitute of Oral Science, Chung Shan Medical University, Taichung City, TaiwanSchool of Dentistry, Chung Shan Medical University, Taichung City, TaiwanSchool of Dentistry, Chung Shan Medical University, Taichung City, TaiwanSchool of Dentistry, Chung Shan Medical University, Taichung City, TaiwanSchool of Dentistry, Chung Shan Medical University, Taichung City, TaiwanBackground/purpose: Bone remodeling is regulated by the replacement of old bone with new bone through sequential osteoclastic resorption and osteoblastic bone formation. In this study, we compare the functional difference in osteoclastogenesis of murine macrophage when applied to the receptor activator of nuclear factor kappa B ligand (RANKL) and osteoprotegerin (OPG) produced by osteoblasts in response to tensile stress in a coculture system. Materials and methods: We examined the secretion of RANKL and OPG at different time points when the osteoblast cells are being cultured under tensile force. In order to confirm whether the media produced by the osteoblast cells under tensile force induce osteoclastogenesis or not, murine macrophage (RAW264.7 cells) were cocultured with osteoblasts. Results: In both mono- and coculture systems, the two cell types cultured under tensile force and in normal environment showed no significant differences (P > 0.05) at any time. However, in the coculture system, the production of tartrate-resistant acid phosphatase (TRAP) in RAW264.7 cells under tensile force was found to increase significantly (P < 0.05; by 2.27-, 3.00-, or 3.27-fold on Day 3, Day 7, or Day 15, respectively) when compared with the corresponding TRAP activity in RAW264.7 cells in normal environment. Moreover, the results indicate that the tensile force upregulated the secretion of RANKL and inhibited OPG synthesis. Therefore, RAW264.7 cells appear to increase their production of TRAP in the media of osteoblasts under tensile force, increasing TRAP activity by nearly 2.8 times compared to that in the media of osteoblasts in a normal environment for 3 days. Conclusion: These results suggest that osteoblasts influence the secretion of RANKL more than OPG when stimulated with osteoclastogenesis via RANKL under tensile force.http://www.sciencedirect.com/science/article/pii/S1991790213001736macrophagesosteoblastosteoprotegerinreceptor activator of nuclear factor kappa B ligandtensile forcetartrate-resistant acid phosphatase |
| spellingShingle | Yi-Jyun Chen Ming-You Shie Chi-Jr Hung Shiau-Lee Liu Tsui-Hsien Huang Chia-Tze Kao Osteoblasts subjected to tensile force induce osteoclastic differentiation of murine macrophages in a coculture system Journal of Dental Sciences macrophages osteoblast osteoprotegerin receptor activator of nuclear factor kappa B ligand tensile force tartrate-resistant acid phosphatase |
| title | Osteoblasts subjected to tensile force induce osteoclastic differentiation of murine macrophages in a coculture system |
| title_full | Osteoblasts subjected to tensile force induce osteoclastic differentiation of murine macrophages in a coculture system |
| title_fullStr | Osteoblasts subjected to tensile force induce osteoclastic differentiation of murine macrophages in a coculture system |
| title_full_unstemmed | Osteoblasts subjected to tensile force induce osteoclastic differentiation of murine macrophages in a coculture system |
| title_short | Osteoblasts subjected to tensile force induce osteoclastic differentiation of murine macrophages in a coculture system |
| title_sort | osteoblasts subjected to tensile force induce osteoclastic differentiation of murine macrophages in a coculture system |
| topic | macrophages osteoblast osteoprotegerin receptor activator of nuclear factor kappa B ligand tensile force tartrate-resistant acid phosphatase |
| url | http://www.sciencedirect.com/science/article/pii/S1991790213001736 |
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