A Target-Site Mutation Confers Cross-Resistance to ALS-Inhibiting Herbicides in <i>Erigeron sumatrensis</i> from Brazil
Cases of weed resistant to herbicides have changed the dynamics of agricultural areas in Brazil, and in recent years, <i>Erigeron</i> species have caused major problems to farmers in the country, mainly in relation to the ineffectiveness of herbicide treatments used. The objective of thi...
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MDPI AG
2022-02-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/11/4/467 |
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| author | Vanessa Vital Silva Rafael Mendes Andreia Suzukawa Fernando Adegas Francismar Marcelino-Guimaraes Rubem Oliveira |
| author_facet | Vanessa Vital Silva Rafael Mendes Andreia Suzukawa Fernando Adegas Francismar Marcelino-Guimaraes Rubem Oliveira |
| author_sort | Vanessa Vital Silva |
| collection | DOAJ |
| description | Cases of weed resistant to herbicides have changed the dynamics of agricultural areas in Brazil, and in recent years, <i>Erigeron</i> species have caused major problems to farmers in the country, mainly in relation to the ineffectiveness of herbicide treatments used. The objective of this study was to confirm the cross-resistance to ALS inhibitors in populations of <i>Erigeron sumatrensis</i> as well as to investigate the existence of mutations in the site of action of ALS-inhibiting herbicides. To do this, 30 populations collected in the 2016/2017 crop season were grown in a greenhouse. Dose–response (chlorimuron-ethyl and cloransulam-methyl), inhibition of cytochrome P-450 with malathion, and ALS gene sequencing experiments were carried out in the F1 generations of two fleabane populations. The results proved the cross-resistance to chlorimuron-ethyl and cloransulam-methyl herbicides applied in the post-emergence of the resistant population of <i>E</i>. <i>sumatrensis</i>. The higher activity of P450 enzymes is unlikely responsible for the resistance of the population studied. The resistance mechanism found in R was the target site mutation Pro197Ser at the ALS gene. This is the first study in Brazil to identify a target-site change as a survival mechanism in <i>E</i>. <i>sumatrensis</i> for the resistance to ALS-inhibiting herbicides. |
| first_indexed | 2024-03-09T21:13:34Z |
| format | Article |
| id | doaj.art-f893b7cba8f740bcbfbbaa95d9eae418 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T21:13:34Z |
| publishDate | 2022-02-01 |
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| series | Plants |
| spelling | doaj.art-f893b7cba8f740bcbfbbaa95d9eae4182023-11-23T21:41:52ZengMDPI AGPlants2223-77472022-02-0111446710.3390/plants11040467A Target-Site Mutation Confers Cross-Resistance to ALS-Inhibiting Herbicides in <i>Erigeron sumatrensis</i> from BrazilVanessa Vital Silva0Rafael Mendes1Andreia Suzukawa2Fernando Adegas3Francismar Marcelino-Guimaraes4Rubem Oliveira5Department of Agronomy, State University of Maringá, Maringá 87020-900, BrazilDepartment of Agronomy, State University of Maringá, Maringá 87020-900, BrazilCrop and Soil Science Department, Oregon State University, Corvallis, OR 97331, USAEmbrapa Soybean, Londrina 86001-970, BrazilEmbrapa Soybean, Londrina 86001-970, BrazilDepartment of Agronomy, State University of Maringá, Maringá 87020-900, BrazilCases of weed resistant to herbicides have changed the dynamics of agricultural areas in Brazil, and in recent years, <i>Erigeron</i> species have caused major problems to farmers in the country, mainly in relation to the ineffectiveness of herbicide treatments used. The objective of this study was to confirm the cross-resistance to ALS inhibitors in populations of <i>Erigeron sumatrensis</i> as well as to investigate the existence of mutations in the site of action of ALS-inhibiting herbicides. To do this, 30 populations collected in the 2016/2017 crop season were grown in a greenhouse. Dose–response (chlorimuron-ethyl and cloransulam-methyl), inhibition of cytochrome P-450 with malathion, and ALS gene sequencing experiments were carried out in the F1 generations of two fleabane populations. The results proved the cross-resistance to chlorimuron-ethyl and cloransulam-methyl herbicides applied in the post-emergence of the resistant population of <i>E</i>. <i>sumatrensis</i>. The higher activity of P450 enzymes is unlikely responsible for the resistance of the population studied. The resistance mechanism found in R was the target site mutation Pro197Ser at the ALS gene. This is the first study in Brazil to identify a target-site change as a survival mechanism in <i>E</i>. <i>sumatrensis</i> for the resistance to ALS-inhibiting herbicides.https://www.mdpi.com/2223-7747/11/4/467Sumatran fleabaneacetolactate synthasesulfonylureasimidazolinonestriazolopyrimidinesresistance factor |
| spellingShingle | Vanessa Vital Silva Rafael Mendes Andreia Suzukawa Fernando Adegas Francismar Marcelino-Guimaraes Rubem Oliveira A Target-Site Mutation Confers Cross-Resistance to ALS-Inhibiting Herbicides in <i>Erigeron sumatrensis</i> from Brazil Plants Sumatran fleabane acetolactate synthase sulfonylureas imidazolinones triazolopyrimidines resistance factor |
| title | A Target-Site Mutation Confers Cross-Resistance to ALS-Inhibiting Herbicides in <i>Erigeron sumatrensis</i> from Brazil |
| title_full | A Target-Site Mutation Confers Cross-Resistance to ALS-Inhibiting Herbicides in <i>Erigeron sumatrensis</i> from Brazil |
| title_fullStr | A Target-Site Mutation Confers Cross-Resistance to ALS-Inhibiting Herbicides in <i>Erigeron sumatrensis</i> from Brazil |
| title_full_unstemmed | A Target-Site Mutation Confers Cross-Resistance to ALS-Inhibiting Herbicides in <i>Erigeron sumatrensis</i> from Brazil |
| title_short | A Target-Site Mutation Confers Cross-Resistance to ALS-Inhibiting Herbicides in <i>Erigeron sumatrensis</i> from Brazil |
| title_sort | target site mutation confers cross resistance to als inhibiting herbicides in i erigeron sumatrensis i from brazil |
| topic | Sumatran fleabane acetolactate synthase sulfonylureas imidazolinones triazolopyrimidines resistance factor |
| url | https://www.mdpi.com/2223-7747/11/4/467 |
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