Fermentation quality, aerobic stability, and microbiome structure and function of Caragana korshinskii silage inoculated with/without Lactobacillus rhamnosus or Lactobacillus buchneri
Caragana korshinskii is a forage shrub species with high-protein content that has been extensively used to alleviate feed shortages for ruminants in northern China. Herein, we investigated the effects of Lactobacillus rhamnosus and Lactobacillus buchneri on the fermentation quality, aerobic stabilit...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-09-01
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| Series: | Frontiers in Sustainable Food Systems |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fsufs.2023.1255936/full |
| _version_ | 1797679267738288128 |
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| author | Baiyila Wu Juanjuan Ai Tiyu Li Weize Qin Zongfu Hu Tuya Siqin Tiemei Wu Chao Wang Huaxin Niu |
| author_facet | Baiyila Wu Juanjuan Ai Tiyu Li Weize Qin Zongfu Hu Tuya Siqin Tiemei Wu Chao Wang Huaxin Niu |
| author_sort | Baiyila Wu |
| collection | DOAJ |
| description | Caragana korshinskii is a forage shrub species with high-protein content that has been extensively used to alleviate feed shortages for ruminants in northern China. Herein, we investigated the effects of Lactobacillus rhamnosus and Lactobacillus buchneri on the fermentation quality, aerobic stability, and microbiome composition and the predicted functional characteristics of C. korshinskii silage. C. korshinskii silages were inoculated with and without L. rhamnosus or L. buchneri. After 14 and 56 days of ensiling, the aerobic stability was determined. The results revealed that after 14 and 56 days of ensiling, L. rhamnosus- and L. buchneri-inoculated silage exhibited increased acetic acid and lactic acid contents, whereas the pH and 2,3-butanediol and butyric acid contents were decreased compared with those of the control silage. The control silages that were opened at 14 and 56 d, deteriorated during the aerobic stability test, whereas silages inoculated with L. rhamnosus and L. buchneri did not exhibit any aerobic deterioration. The control silage showed an increased Clostridium and Bacillus abundance, whereas Lactobacillus abundance decreased compared with L. rhamnosus- and L. buchneri-inoculated silages, following the 7 days of aerobic exposure. The fermentation parameters were associated with microbial communities, including Lactobacillus, Pedicoccus, Weissella, Clostridium, and Bacillus. Carbohydrate and amino acid metabolisms in the control silage decreased after 7 days of aerobic exposure compared with lactic acid bacteria-inoculated silages. To conclude, next-generation sequencing combined with 16S ribosomal RNA gene-predicted functional analyses might provide new information about the silage quality during fermentation and the aerobic stability. |
| first_indexed | 2024-03-11T23:12:03Z |
| format | Article |
| id | doaj.art-f894df2ba5a241a69c7b698773decd3e |
| institution | Directory Open Access Journal |
| issn | 2571-581X |
| language | English |
| last_indexed | 2024-03-11T23:12:03Z |
| publishDate | 2023-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Sustainable Food Systems |
| spelling | doaj.art-f894df2ba5a241a69c7b698773decd3e2023-09-21T07:55:04ZengFrontiers Media S.A.Frontiers in Sustainable Food Systems2571-581X2023-09-01710.3389/fsufs.2023.12559361255936Fermentation quality, aerobic stability, and microbiome structure and function of Caragana korshinskii silage inoculated with/without Lactobacillus rhamnosus or Lactobacillus buchneriBaiyila Wu0Juanjuan Ai1Tiyu Li2Weize Qin3Zongfu Hu4Tuya Siqin5Tiemei Wu6Chao Wang7Huaxin Niu8College of Animal Science and Technology, Inner Mongolia Minzu University, Tongliao, ChinaMedical College, Inner Mongolia Minzu University, Tongliao, ChinaCollege of Animal Science and Technology, Inner Mongolia Minzu University, Tongliao, ChinaCollege of Animal Science and Technology, Inner Mongolia Minzu University, Tongliao, ChinaCollege of Animal Science and Technology, Inner Mongolia Minzu University, Tongliao, ChinaCollege of Animal Science and Technology, Inner Mongolia Minzu University, Tongliao, ChinaCollege of Animal Science and Technology, Inner Mongolia Minzu University, Tongliao, ChinaInner Mongolia Academy of Agriculture and Animal Husbandry Science, Hohhot, ChinaCollege of Animal Science and Technology, Inner Mongolia Minzu University, Tongliao, ChinaCaragana korshinskii is a forage shrub species with high-protein content that has been extensively used to alleviate feed shortages for ruminants in northern China. Herein, we investigated the effects of Lactobacillus rhamnosus and Lactobacillus buchneri on the fermentation quality, aerobic stability, and microbiome composition and the predicted functional characteristics of C. korshinskii silage. C. korshinskii silages were inoculated with and without L. rhamnosus or L. buchneri. After 14 and 56 days of ensiling, the aerobic stability was determined. The results revealed that after 14 and 56 days of ensiling, L. rhamnosus- and L. buchneri-inoculated silage exhibited increased acetic acid and lactic acid contents, whereas the pH and 2,3-butanediol and butyric acid contents were decreased compared with those of the control silage. The control silages that were opened at 14 and 56 d, deteriorated during the aerobic stability test, whereas silages inoculated with L. rhamnosus and L. buchneri did not exhibit any aerobic deterioration. The control silage showed an increased Clostridium and Bacillus abundance, whereas Lactobacillus abundance decreased compared with L. rhamnosus- and L. buchneri-inoculated silages, following the 7 days of aerobic exposure. The fermentation parameters were associated with microbial communities, including Lactobacillus, Pedicoccus, Weissella, Clostridium, and Bacillus. Carbohydrate and amino acid metabolisms in the control silage decreased after 7 days of aerobic exposure compared with lactic acid bacteria-inoculated silages. To conclude, next-generation sequencing combined with 16S ribosomal RNA gene-predicted functional analyses might provide new information about the silage quality during fermentation and the aerobic stability.https://www.frontiersin.org/articles/10.3389/fsufs.2023.1255936/fullCaragana korshinskii silagefermentation productsLactobacillus rhamnosusLactobacillus buchnerimicrobial communitymicrobial function characteristics |
| spellingShingle | Baiyila Wu Juanjuan Ai Tiyu Li Weize Qin Zongfu Hu Tuya Siqin Tiemei Wu Chao Wang Huaxin Niu Fermentation quality, aerobic stability, and microbiome structure and function of Caragana korshinskii silage inoculated with/without Lactobacillus rhamnosus or Lactobacillus buchneri Frontiers in Sustainable Food Systems Caragana korshinskii silage fermentation products Lactobacillus rhamnosus Lactobacillus buchneri microbial community microbial function characteristics |
| title | Fermentation quality, aerobic stability, and microbiome structure and function of Caragana korshinskii silage inoculated with/without Lactobacillus rhamnosus or Lactobacillus buchneri |
| title_full | Fermentation quality, aerobic stability, and microbiome structure and function of Caragana korshinskii silage inoculated with/without Lactobacillus rhamnosus or Lactobacillus buchneri |
| title_fullStr | Fermentation quality, aerobic stability, and microbiome structure and function of Caragana korshinskii silage inoculated with/without Lactobacillus rhamnosus or Lactobacillus buchneri |
| title_full_unstemmed | Fermentation quality, aerobic stability, and microbiome structure and function of Caragana korshinskii silage inoculated with/without Lactobacillus rhamnosus or Lactobacillus buchneri |
| title_short | Fermentation quality, aerobic stability, and microbiome structure and function of Caragana korshinskii silage inoculated with/without Lactobacillus rhamnosus or Lactobacillus buchneri |
| title_sort | fermentation quality aerobic stability and microbiome structure and function of caragana korshinskii silage inoculated with without lactobacillus rhamnosus or lactobacillus buchneri |
| topic | Caragana korshinskii silage fermentation products Lactobacillus rhamnosus Lactobacillus buchneri microbial community microbial function characteristics |
| url | https://www.frontiersin.org/articles/10.3389/fsufs.2023.1255936/full |
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