Autologous adipose-derived stem cell sheets enhance the strength of intestinal anastomosis
Objective: Adipose-derived stem cells (ASCs) are capable of multiple differentiation pathways, imparting immunomodulatory effects, and secreting factors that are important for wound healing. These characteristics can be exploited to decrease the incidence of anastomotic leakage. Methods: In order to...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2017-12-01
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| Series: | Regenerative Therapy |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S235232041730007X |
| _version_ | 1819294692416684032 |
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| author | Yasuhiro Maruya Nobuo Kanai Shinichiro Kobayashi Kurodo Koshino Teruo Okano Susumu Eguchi Masayuki Yamato |
| author_facet | Yasuhiro Maruya Nobuo Kanai Shinichiro Kobayashi Kurodo Koshino Teruo Okano Susumu Eguchi Masayuki Yamato |
| author_sort | Yasuhiro Maruya |
| collection | DOAJ |
| description | Objective: Adipose-derived stem cells (ASCs) are capable of multiple differentiation pathways, imparting immunomodulatory effects, and secreting factors that are important for wound healing. These characteristics can be exploited to decrease the incidence of anastomotic leakage.
Methods: In order to delay local wound healing at the anastomotic site, we induced ischemia in a portion of porcine small intestine by ligating vessels. Then, we injected mitomycin C into the serosa of the small intestine above the ligated vessels. Anastomotic sites were created by 2 cm incisions made in the opposite mesenteric area. ASCs were isolated from the porcine subcutaneous fat tissues and expanded under culture conditions. ASCs were trypsinized and seeded on temperature-responsive dishes and cultured to form confluent sheets. Three ASC sheets were transplanted onto the serous membrane after suturing. The extent of anastomotic wound healing was evaluated by bursting pressure, hydroxyproline content, and mRNA expression of collagen-1 alpha1 and collagen-3 alpha1.
Results: We found that transplantation of ASC sheets increased anastomotic site bursting pressure. Additionally, transplantation of ASC sheets increased the hydroxyproline content of the anastomoses. Furthermore, transplantation of ASC sheets increased mRNA expression of collagen-1 alpha1 and collagen-3 alpha1.
Conclusions: Our findings showed that transplantation of autologous ASC sheets enhanced collagen synthesis and anastomotic strength. Further studies are necessary to identify substances that, in combination with ASC sheets, might enhance collagen synthesis and healing in sites of anastomosis. |
| first_indexed | 2024-12-24T04:30:22Z |
| format | Article |
| id | doaj.art-f895acd9e9f5449f8a7cf898de1278d7 |
| institution | Directory Open Access Journal |
| issn | 2352-3204 |
| language | English |
| last_indexed | 2024-12-24T04:30:22Z |
| publishDate | 2017-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Regenerative Therapy |
| spelling | doaj.art-f895acd9e9f5449f8a7cf898de1278d72022-12-21T17:15:28ZengElsevierRegenerative Therapy2352-32042017-12-017C243310.1016/j.reth.2017.06.004Autologous adipose-derived stem cell sheets enhance the strength of intestinal anastomosisYasuhiro Maruya0Nobuo Kanai1Shinichiro Kobayashi2Kurodo Koshino3Teruo Okano4Susumu Eguchi5Masayuki Yamato6Department of Surgery Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, JapanInstitute of Advanced Biomedical Engineering and Science Tokyo Women's Medical University, Tokyo, JapanDepartment of Surgery Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, JapanInstitute of Advanced Biomedical Engineering and Science Tokyo Women's Medical University, Tokyo, JapanInstitute of Advanced Biomedical Engineering and Science Tokyo Women's Medical University, Tokyo, JapanDepartment of Surgery Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, JapanInstitute of Advanced Biomedical Engineering and Science Tokyo Women's Medical University, Tokyo, JapanObjective: Adipose-derived stem cells (ASCs) are capable of multiple differentiation pathways, imparting immunomodulatory effects, and secreting factors that are important for wound healing. These characteristics can be exploited to decrease the incidence of anastomotic leakage. Methods: In order to delay local wound healing at the anastomotic site, we induced ischemia in a portion of porcine small intestine by ligating vessels. Then, we injected mitomycin C into the serosa of the small intestine above the ligated vessels. Anastomotic sites were created by 2 cm incisions made in the opposite mesenteric area. ASCs were isolated from the porcine subcutaneous fat tissues and expanded under culture conditions. ASCs were trypsinized and seeded on temperature-responsive dishes and cultured to form confluent sheets. Three ASC sheets were transplanted onto the serous membrane after suturing. The extent of anastomotic wound healing was evaluated by bursting pressure, hydroxyproline content, and mRNA expression of collagen-1 alpha1 and collagen-3 alpha1. Results: We found that transplantation of ASC sheets increased anastomotic site bursting pressure. Additionally, transplantation of ASC sheets increased the hydroxyproline content of the anastomoses. Furthermore, transplantation of ASC sheets increased mRNA expression of collagen-1 alpha1 and collagen-3 alpha1. Conclusions: Our findings showed that transplantation of autologous ASC sheets enhanced collagen synthesis and anastomotic strength. Further studies are necessary to identify substances that, in combination with ASC sheets, might enhance collagen synthesis and healing in sites of anastomosis.http://www.sciencedirect.com/science/article/pii/S235232041730007XAdipose-derived stem cellsPrevent anastomotic leakage |
| spellingShingle | Yasuhiro Maruya Nobuo Kanai Shinichiro Kobayashi Kurodo Koshino Teruo Okano Susumu Eguchi Masayuki Yamato Autologous adipose-derived stem cell sheets enhance the strength of intestinal anastomosis Regenerative Therapy Adipose-derived stem cells Prevent anastomotic leakage |
| title | Autologous adipose-derived stem cell sheets enhance the strength of intestinal anastomosis |
| title_full | Autologous adipose-derived stem cell sheets enhance the strength of intestinal anastomosis |
| title_fullStr | Autologous adipose-derived stem cell sheets enhance the strength of intestinal anastomosis |
| title_full_unstemmed | Autologous adipose-derived stem cell sheets enhance the strength of intestinal anastomosis |
| title_short | Autologous adipose-derived stem cell sheets enhance the strength of intestinal anastomosis |
| title_sort | autologous adipose derived stem cell sheets enhance the strength of intestinal anastomosis |
| topic | Adipose-derived stem cells Prevent anastomotic leakage |
| url | http://www.sciencedirect.com/science/article/pii/S235232041730007X |
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