Highlights on the capacities of "Gel-based" proteomics
<p>Abstract</p> <p>Gel-based proteomic is the most popular and versatile method of global protein separation and quantification. This is a mature approach to screen the protein expression at the large scale, and a cheaper approach as compared with gel-free proteomics. Based on two...
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| Format: | Article |
| Language: | English |
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BMC
2010-04-01
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| Series: | Proteome Science |
| Online Access: | http://www.proteomesci.com/content/8/1/23 |
| _version_ | 1818919401564405760 |
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| author | Chevalier François |
| author_facet | Chevalier François |
| author_sort | Chevalier François |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Gel-based proteomic is the most popular and versatile method of global protein separation and quantification. This is a mature approach to screen the protein expression at the large scale, and a cheaper approach as compared with gel-free proteomics. Based on two independent biochemical characteristics of proteins, two-dimensional electrophoresis combines isoelectric focusing, which separates proteins according to their isoelectric point, and SDS-PAGE, which separates them further according to their molecular mass. The next typical steps of the flow of gel-based proteomics are spots visualization and evaluation, expression analysis and finally protein identification by mass spectrometry. For the study of differentially expressed proteins, two-dimensional electrophoresis allows simultaneously to detect, quantify and compare up to thousand protein spots isoforms, including post-translational modifications, in the same gel and in a wide range of biological systems. In this review article, the limits, benefits, and perspectives of gel-based proteomic approaches are discussed using concrete examples.</p> |
| first_indexed | 2024-12-20T01:05:16Z |
| format | Article |
| id | doaj.art-f8a1ada7858b4b7485c8059b7dff9128 |
| institution | Directory Open Access Journal |
| issn | 1477-5956 |
| language | English |
| last_indexed | 2024-12-20T01:05:16Z |
| publishDate | 2010-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Proteome Science |
| spelling | doaj.art-f8a1ada7858b4b7485c8059b7dff91282022-12-21T19:58:53ZengBMCProteome Science1477-59562010-04-01812310.1186/1477-5956-8-23Highlights on the capacities of "Gel-based" proteomicsChevalier François<p>Abstract</p> <p>Gel-based proteomic is the most popular and versatile method of global protein separation and quantification. This is a mature approach to screen the protein expression at the large scale, and a cheaper approach as compared with gel-free proteomics. Based on two independent biochemical characteristics of proteins, two-dimensional electrophoresis combines isoelectric focusing, which separates proteins according to their isoelectric point, and SDS-PAGE, which separates them further according to their molecular mass. The next typical steps of the flow of gel-based proteomics are spots visualization and evaluation, expression analysis and finally protein identification by mass spectrometry. For the study of differentially expressed proteins, two-dimensional electrophoresis allows simultaneously to detect, quantify and compare up to thousand protein spots isoforms, including post-translational modifications, in the same gel and in a wide range of biological systems. In this review article, the limits, benefits, and perspectives of gel-based proteomic approaches are discussed using concrete examples.</p>http://www.proteomesci.com/content/8/1/23 |
| spellingShingle | Chevalier François Highlights on the capacities of "Gel-based" proteomics Proteome Science |
| title | Highlights on the capacities of "Gel-based" proteomics |
| title_full | Highlights on the capacities of "Gel-based" proteomics |
| title_fullStr | Highlights on the capacities of "Gel-based" proteomics |
| title_full_unstemmed | Highlights on the capacities of "Gel-based" proteomics |
| title_short | Highlights on the capacities of "Gel-based" proteomics |
| title_sort | highlights on the capacities of gel based proteomics |
| url | http://www.proteomesci.com/content/8/1/23 |
| work_keys_str_mv | AT chevalierfrancois highlightsonthecapacitiesofgelbasedproteomics |