HPV-related oropharyngeal cancer prevalence in a middle eastern population using E6/E7 PCR
Abstract Background Given the paucity of data and widely variable rates that have been reported, the main objective of this study was to examine the prevalence of HPV-positivity in oropharyngeal squamous cell carcinoma (OPSCC) in Middle Eastern patients presenting to one of the region’s largest tert...
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| Format: | Article |
| Language: | English |
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BMC
2020-01-01
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| Series: | Infectious Agents and Cancer |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13027-019-0268-z |
| _version_ | 1818725693322690560 |
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| author | Christopher A. Maroun Karine Al Feghali Henri Traboulsi Helene Dabbous Fatmeh Abbas Gabriel Dunya Georges Ziade Rami Mahfouz Bassem Youssef Hani Tamim Fady Geara Ibrahim Khalifeh Roger V. Moukarbel |
| author_facet | Christopher A. Maroun Karine Al Feghali Henri Traboulsi Helene Dabbous Fatmeh Abbas Gabriel Dunya Georges Ziade Rami Mahfouz Bassem Youssef Hani Tamim Fady Geara Ibrahim Khalifeh Roger V. Moukarbel |
| author_sort | Christopher A. Maroun |
| collection | DOAJ |
| description | Abstract Background Given the paucity of data and widely variable rates that have been reported, the main objective of this study was to examine the prevalence of HPV-positivity in oropharyngeal squamous cell carcinoma (OPSCC) in Middle Eastern patients presenting to one of the region’s largest tertiary care centers using polymerase chain reaction (PCR) amplification of the HPV E6/E7 oncogenes, a highly sensitive and specific method of detection. Methods Medical charts and archived pathological specimens were obtained for patients diagnosed with biopsy proven oropharyngeal cancer who presented to the American University of Beirut Medical Center between 1972 and 2017. DNA was extracted from paraffin-embedded specimens and tested for 30 high-risk and low-risk papilloma viruses using the PCR-based EUROarray HPV kit (EuroImmun). Results A total of 57 patients with oropharyngeal cancer were initially identified; only 34 met inclusion/exclusion criteria and were included in the present study. Most patients were males (73.5%) from Lebanon (79.4%). The most common primary tumor site was in the base of tongue (50%), followed by the tonsil (41.2%). The majority of patients (85.3%) tested positive for HPV DNA. Conclusion The prevalence of HPV-positivity amongst Middle Eastern OPSCC patients, specifically those from Lebanon, may be far greater than previously thought. The Lebanese population and other neighboring Middle Eastern countries may require a more vigilant approach towards HPV detection and awareness. On an international level, further research is required to better elucidate non-classical mechanisms of HPV exposure and transmission. |
| first_indexed | 2024-12-17T21:46:22Z |
| format | Article |
| id | doaj.art-f8a2f974a73a4669b32737ba6c03db52 |
| institution | Directory Open Access Journal |
| issn | 1750-9378 |
| language | English |
| last_indexed | 2024-12-17T21:46:22Z |
| publishDate | 2020-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Infectious Agents and Cancer |
| spelling | doaj.art-f8a2f974a73a4669b32737ba6c03db522022-12-21T21:31:28ZengBMCInfectious Agents and Cancer1750-93782020-01-011511710.1186/s13027-019-0268-zHPV-related oropharyngeal cancer prevalence in a middle eastern population using E6/E7 PCRChristopher A. Maroun0Karine Al Feghali1Henri Traboulsi2Helene Dabbous3Fatmeh Abbas4Gabriel Dunya5Georges Ziade6Rami Mahfouz7Bassem Youssef8Hani Tamim9Fady Geara10Ibrahim Khalifeh11Roger V. Moukarbel12Department of Otolaryngology Head and Neck Surgery, American University of Beirut Medical CenterDepartment of Radiation Oncology, American University of Beirut Medical CenterDepartment of Otolaryngology Head and Neck Surgery, American University of Beirut Medical CenterDepartment of Otolaryngology Head and Neck Surgery, American University of Beirut Medical CenterDepartment of Pathology and Laboratory Medicine, American University of Beirut Medical CenterDepartment of Otolaryngology Head and Neck Surgery, American University of Beirut Medical CenterDepartment of Otolaryngology Head and Neck Surgery, American University of Beirut Medical CenterDepartment of Pathology and Laboratory Medicine, American University of Beirut Medical CenterDepartment of Radiation Oncology, American University of Beirut Medical CenterBiostatistics Unit, Clinical Research Institute, Faculty of Medicine, American University of BeirutDepartment of Radiation Oncology, American University of Beirut Medical CenterDepartment of Pathology and Laboratory Medicine, American University of Beirut Medical CenterDepartment of Otolaryngology Head and Neck Surgery, American University of Beirut Medical CenterAbstract Background Given the paucity of data and widely variable rates that have been reported, the main objective of this study was to examine the prevalence of HPV-positivity in oropharyngeal squamous cell carcinoma (OPSCC) in Middle Eastern patients presenting to one of the region’s largest tertiary care centers using polymerase chain reaction (PCR) amplification of the HPV E6/E7 oncogenes, a highly sensitive and specific method of detection. Methods Medical charts and archived pathological specimens were obtained for patients diagnosed with biopsy proven oropharyngeal cancer who presented to the American University of Beirut Medical Center between 1972 and 2017. DNA was extracted from paraffin-embedded specimens and tested for 30 high-risk and low-risk papilloma viruses using the PCR-based EUROarray HPV kit (EuroImmun). Results A total of 57 patients with oropharyngeal cancer were initially identified; only 34 met inclusion/exclusion criteria and were included in the present study. Most patients were males (73.5%) from Lebanon (79.4%). The most common primary tumor site was in the base of tongue (50%), followed by the tonsil (41.2%). The majority of patients (85.3%) tested positive for HPV DNA. Conclusion The prevalence of HPV-positivity amongst Middle Eastern OPSCC patients, specifically those from Lebanon, may be far greater than previously thought. The Lebanese population and other neighboring Middle Eastern countries may require a more vigilant approach towards HPV detection and awareness. On an international level, further research is required to better elucidate non-classical mechanisms of HPV exposure and transmission.https://doi.org/10.1186/s13027-019-0268-zOropharyngeal cancerHead and neck squamous cell carcinomaHuman papilloma virusMiddle EastLebanon |
| spellingShingle | Christopher A. Maroun Karine Al Feghali Henri Traboulsi Helene Dabbous Fatmeh Abbas Gabriel Dunya Georges Ziade Rami Mahfouz Bassem Youssef Hani Tamim Fady Geara Ibrahim Khalifeh Roger V. Moukarbel HPV-related oropharyngeal cancer prevalence in a middle eastern population using E6/E7 PCR Infectious Agents and Cancer Oropharyngeal cancer Head and neck squamous cell carcinoma Human papilloma virus Middle East Lebanon |
| title | HPV-related oropharyngeal cancer prevalence in a middle eastern population using E6/E7 PCR |
| title_full | HPV-related oropharyngeal cancer prevalence in a middle eastern population using E6/E7 PCR |
| title_fullStr | HPV-related oropharyngeal cancer prevalence in a middle eastern population using E6/E7 PCR |
| title_full_unstemmed | HPV-related oropharyngeal cancer prevalence in a middle eastern population using E6/E7 PCR |
| title_short | HPV-related oropharyngeal cancer prevalence in a middle eastern population using E6/E7 PCR |
| title_sort | hpv related oropharyngeal cancer prevalence in a middle eastern population using e6 e7 pcr |
| topic | Oropharyngeal cancer Head and neck squamous cell carcinoma Human papilloma virus Middle East Lebanon |
| url | https://doi.org/10.1186/s13027-019-0268-z |
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