Phenotypic profile and antibiogram of biofilm-producing bacteria isolates from diabetic foot ulcers in Zaria, Nigeria
Background: Diabetic foot ulcers (DFUs) present with high morbidity and reduce patient's quality of life. There is a gross paucity of data on biofilm-producing bacteria in DFU Infection in North-Western Nigeria. The study sought to determine the biofilm-forming ability of bacteria isolates from...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2021-01-01
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| Series: | Nigerian Postgraduate Medical Journal |
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| Online Access: | http://www.npmj.org/article.asp?issn=1117-1936;year=2021;volume=28;issue=4;spage=233;epage=239;aulast=Usman |
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| author | Yahaya Usman Adamu Girei Bakari Idris Nasir Abdullahi Abdurrahman El-Fulaty Ahmad Fatima Sani-Bello Atiene Solomon Sagay Adebola Tolulope Olayinka |
| author_facet | Yahaya Usman Adamu Girei Bakari Idris Nasir Abdullahi Abdurrahman El-Fulaty Ahmad Fatima Sani-Bello Atiene Solomon Sagay Adebola Tolulope Olayinka |
| author_sort | Yahaya Usman |
| collection | DOAJ |
| description | Background: Diabetic foot ulcers (DFUs) present with high morbidity and reduce patient's quality of life. There is a gross paucity of data on biofilm-producing bacteria in DFU Infection in North-Western Nigeria. The study sought to determine the biofilm-forming ability of bacteria isolates from DFUs and determine their antimicrobial susceptibility pattern in Zaria, North-Western Nigeria. Materials and Methods: This hospital-based cross-sectional study of patients with DFUs was conducted from June 2018 to February 2020. Consecutive biopsies were aseptically collected. Bacteria were isolated and identified using a Microgen kit. Biofilm forming ability and antibiogram of isolates were determined using microtitre plate and disk diffusion methods, respectively. Results: Of the 225 participants enrolled, males constituted the majority, 144 (64.0%) with 88 (36.0%) females, the median age of participants was 54 (48–60) years, and the age range was 36–77 years. A total of 172 bacteria were isolated, and 123 (71.5%) were biofilm producers. Staphylococcus aureus (26.7%) was the highest biofilm producer, while Citrobacter freundii and Stenotrophomonas maltophilia were the least biofilm producers, 1 (0.6%) each. A disproportionate resistance pattern was demonstrated among the biofilm and non-biofilm producers against the cephalosporins tested, ceftazidime (68% vs. 18%), ceftriaxone (50% vs. 8.0%) and cefotaxime (21% vs. 0.0%). About 46% and 68% of the biofilm producers were resistant to gentamycin and ciprofloxacin, respectively. While only 2% of the non-biofilm producers were resistant to imipenem, 11% of the biofilm producers were resistant to it. Conclusion: These findings revealed a high proportion of biofilm-producing bacteria and were more resistant than non-biofilm producers. |
| first_indexed | 2024-12-22T06:01:42Z |
| format | Article |
| id | doaj.art-f8a3532f6bcd45d1904746466ce0f8f3 |
| institution | Directory Open Access Journal |
| issn | 1117-1936 2468-6875 |
| language | English |
| last_indexed | 2024-12-22T06:01:42Z |
| publishDate | 2021-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Nigerian Postgraduate Medical Journal |
| spelling | doaj.art-f8a3532f6bcd45d1904746466ce0f8f32022-12-21T18:36:32ZengWolters Kluwer Medknow PublicationsNigerian Postgraduate Medical Journal1117-19362468-68752021-01-0128423323910.4103/npmj.npmj_552_21Phenotypic profile and antibiogram of biofilm-producing bacteria isolates from diabetic foot ulcers in Zaria, NigeriaYahaya UsmanAdamu Girei BakariIdris Nasir AbdullahiAbdurrahman El-Fulaty AhmadFatima Sani-BelloAtiene Solomon SagayAdebola Tolulope OlayinkaBackground: Diabetic foot ulcers (DFUs) present with high morbidity and reduce patient's quality of life. There is a gross paucity of data on biofilm-producing bacteria in DFU Infection in North-Western Nigeria. The study sought to determine the biofilm-forming ability of bacteria isolates from DFUs and determine their antimicrobial susceptibility pattern in Zaria, North-Western Nigeria. Materials and Methods: This hospital-based cross-sectional study of patients with DFUs was conducted from June 2018 to February 2020. Consecutive biopsies were aseptically collected. Bacteria were isolated and identified using a Microgen kit. Biofilm forming ability and antibiogram of isolates were determined using microtitre plate and disk diffusion methods, respectively. Results: Of the 225 participants enrolled, males constituted the majority, 144 (64.0%) with 88 (36.0%) females, the median age of participants was 54 (48–60) years, and the age range was 36–77 years. A total of 172 bacteria were isolated, and 123 (71.5%) were biofilm producers. Staphylococcus aureus (26.7%) was the highest biofilm producer, while Citrobacter freundii and Stenotrophomonas maltophilia were the least biofilm producers, 1 (0.6%) each. A disproportionate resistance pattern was demonstrated among the biofilm and non-biofilm producers against the cephalosporins tested, ceftazidime (68% vs. 18%), ceftriaxone (50% vs. 8.0%) and cefotaxime (21% vs. 0.0%). About 46% and 68% of the biofilm producers were resistant to gentamycin and ciprofloxacin, respectively. While only 2% of the non-biofilm producers were resistant to imipenem, 11% of the biofilm producers were resistant to it. Conclusion: These findings revealed a high proportion of biofilm-producing bacteria and were more resistant than non-biofilm producers.http://www.npmj.org/article.asp?issn=1117-1936;year=2021;volume=28;issue=4;spage=233;epage=239;aulast=Usmanantibiogrambacteriabiofilmsdiabetic foot ulcersnigeriazaria |
| spellingShingle | Yahaya Usman Adamu Girei Bakari Idris Nasir Abdullahi Abdurrahman El-Fulaty Ahmad Fatima Sani-Bello Atiene Solomon Sagay Adebola Tolulope Olayinka Phenotypic profile and antibiogram of biofilm-producing bacteria isolates from diabetic foot ulcers in Zaria, Nigeria Nigerian Postgraduate Medical Journal antibiogram bacteria biofilms diabetic foot ulcers nigeria zaria |
| title | Phenotypic profile and antibiogram of biofilm-producing bacteria isolates from diabetic foot ulcers in Zaria, Nigeria |
| title_full | Phenotypic profile and antibiogram of biofilm-producing bacteria isolates from diabetic foot ulcers in Zaria, Nigeria |
| title_fullStr | Phenotypic profile and antibiogram of biofilm-producing bacteria isolates from diabetic foot ulcers in Zaria, Nigeria |
| title_full_unstemmed | Phenotypic profile and antibiogram of biofilm-producing bacteria isolates from diabetic foot ulcers in Zaria, Nigeria |
| title_short | Phenotypic profile and antibiogram of biofilm-producing bacteria isolates from diabetic foot ulcers in Zaria, Nigeria |
| title_sort | phenotypic profile and antibiogram of biofilm producing bacteria isolates from diabetic foot ulcers in zaria nigeria |
| topic | antibiogram bacteria biofilms diabetic foot ulcers nigeria zaria |
| url | http://www.npmj.org/article.asp?issn=1117-1936;year=2021;volume=28;issue=4;spage=233;epage=239;aulast=Usman |
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